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A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A

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  • A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A

    Influenza Other Respir Viruses. 2016 Jul 12. doi: 10.1111/irv.12408. [Epub ahead of print]
    A comparison of hemagglutination inhibition and neutralization assays for characterizing immunity to seasonal influenza A.

    Truelove S1, Zhu H2,3, Lessler J1, Riley S4, Read JM5, Wang S6, Kwok KO3, Guan Y2,3, Jiang CQ6, Cummings DA1,7,8.
    Author information

    Abstract

    BACKGROUND:

    Serum antibody to influenza can be used to identify past exposure and measure current immune status. The two most common methods for measuring this are the hemagglutination inhibition assay (HI) and the viral neutralization assay (NT), which have not been systematically compared for a large number of influenza viruses.
    METHODS:

    151 study participants from near Guangzhou, China were enrolled in 2009 and provided serum. HI and NT assays were performed for 12 historic and recently circulating strains of seasonal influenza A. We compared titers using Spearman correlation and fit models to predict NT using HI results.
    RESULTS:

    We observed high positive mean correlation between HI and NT assays (Spearman's rank correlation, rho=0.86) across all strains. Correlation was highest within subtypes and within close proximity in time. Overall, an HI=20 corresponded to NT=10, and HI=40 corresponded to NT=20. Linear regression of log(NT) on log(HI) was statistically significant, with age modifying this relationship. Strain-specific area under a curve (AUC) indicated good accuracy (>80%) for predicting NT with HI.
    CONCLUSIONS:

    While we found high overall correspondence of titers between NT and HI assays for seasonal influenza A, no exact equivalence between assays could be determined. This was further complicated by correspondence between titers changing with age. These findings support generalized comparison of results between assays and give further support for use of the hemagglutination inhibition assay over the more resource intensive viral neutralization assay for seasonal influenza A, though attention should be given to the effect of age on these assays. This article is protected by copyright. All rights reserved.
    This article is protected by copyright. All rights reserved.


    KEYWORDS:

    cross protection; epidemiology; hemagglutination inhibition test; immunity; influenza; microneutralization test; neutralization test

    PMID: 27406695 DOI: 10.1111/irv.12408
    [PubMed - as supplied by publisher] Free full text
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