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Biotechnol Bioeng. 2016 Mar 22. doi: 10.1002/bit.25982. [Epub ahead of print]
Detection of influenza virus using peroxidase-mimic of gold nanoparticles.
Ahmed SR1, Kim J2, Suzuki T3, Lee J4, Park EY5,6.
Author information
Abstract
A modified enzyme-linked immunosorbent assay (ELISA) with nanomaterials is an effective and powerful method to amplify the signal and reduce the cost of detecting and measuring trace biomarkers or proteins. In this study, an ultra-sensitive colorimetric immunoassay was designed, and its ability to detect influenza viruses using positively charged gold nanoparticles ((+)Au NPs) was assessed as a possible role for peroxidase-mimic inorganic enzymes. This method detected influenza virus A (H1N1) with a linear range up to 10 pg mL-1 and clinically isolated influenza virus A (H3N2) up to 10 plaque forming units (PFU) mL-1 , where its sensitivity improved to 500-fold higher than that of commercial virus kits. The sensitivity of this proposed method was not declined even though in complex biological media in compared to conventional ELISA. These results revealed that the (+)AuNP-based colorimetric immunoassay could be suitable for lab-on-a-chip device and open new opportunities for clinical protein diagnostics. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
gold nanoparticles; immunoassay; influenza virus; peroxidase-mimic enzymatic reaction
PMID: 27002303 [PubMed - as supplied by publisher]
Detection of influenza virus using peroxidase-mimic of gold nanoparticles.
Ahmed SR1, Kim J2, Suzuki T3, Lee J4, Park EY5,6.
Author information
Abstract
A modified enzyme-linked immunosorbent assay (ELISA) with nanomaterials is an effective and powerful method to amplify the signal and reduce the cost of detecting and measuring trace biomarkers or proteins. In this study, an ultra-sensitive colorimetric immunoassay was designed, and its ability to detect influenza viruses using positively charged gold nanoparticles ((+)Au NPs) was assessed as a possible role for peroxidase-mimic inorganic enzymes. This method detected influenza virus A (H1N1) with a linear range up to 10 pg mL-1 and clinically isolated influenza virus A (H3N2) up to 10 plaque forming units (PFU) mL-1 , where its sensitivity improved to 500-fold higher than that of commercial virus kits. The sensitivity of this proposed method was not declined even though in complex biological media in compared to conventional ELISA. These results revealed that the (+)AuNP-based colorimetric immunoassay could be suitable for lab-on-a-chip device and open new opportunities for clinical protein diagnostics. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
gold nanoparticles; immunoassay; influenza virus; peroxidase-mimic enzymatic reaction
PMID: 27002303 [PubMed - as supplied by publisher]