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Diagn Microbiol Infect Dis. 2016 Mar 7. pii: S0732-8893(16)30005-0. doi: 10.1016/j.diagmicrobio.2016.02.004. [Epub ahead of print]
Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season.
Overmeire Y1, Vanlaere E1, Hombrouck A2, De Beenhouwer H3, Simons G4, Brink A4, Van den Abeele AM1, Verfaillie C1, Van Acker J5.
Author information
Abstract
The 2014-2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.
Copyright ? 2016 Elsevier Inc. All rights reserved.
KEYWORDS:
Antigenic drift; Influenza; Laboratory; Laboratory surveillance; Molecular methods
PMID: 26964723 [PubMed - as supplied by publisher]
Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season.
Overmeire Y1, Vanlaere E1, Hombrouck A2, De Beenhouwer H3, Simons G4, Brink A4, Van den Abeele AM1, Verfaillie C1, Van Acker J5.
Author information
Abstract
The 2014-2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.
Copyright ? 2016 Elsevier Inc. All rights reserved.
KEYWORDS:
Antigenic drift; Influenza; Laboratory; Laboratory surveillance; Molecular methods
PMID: 26964723 [PubMed - as supplied by publisher]