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J Virol Methods. 2009 Jul 31. [Epub ahead of print]
Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent.
Sails AD, Saunders D, Airs S, Roberts D, Eltringham G, Magee JG. - Health Protection Agency Newcastle Laboratory, Institute of Pathology, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne, NE4 6BE, UK.
Two rapid real-time RT-PCR assays, specific for respiratory syncytial virus (RSV) and Influenza A and B, were evaluated for the detection of these viruses in clinical respiratory samples. The RSV assay was applied to 100 samples and the Influenza A and B assay applied to 96 samples all of which had been tested previously by an "in-house" multiplex real-time PCR assay. Forty three samples were negative for RSV by both methods and 56 samples were positive by both methods. One sample was negative by the new RSV assay although it was positive for RSV A by the "in-house" test. Thirty nine samples were negative for influenza virus by both methods and 55 samples were positive by both assays. Two samples were negative by the new influenza assay however they were positive by the "in-house" influenza assay. The new assays did not cross react with samples containing other viruses including parainfluenza 1, 2,& 4; human metapnuemovirus; coronavirus 229E, NL63, OC43; rhinovirus; adenovirus; bocavirus and had a specificity of 100% and a sensitivity of 98.2% for RSV and 96.5% for influenza respectively. The results of this study demonstrate that the new assays are specific and sensitive for the detection of RSV and influenza viruses in clinical samples.
PMID: 19651159 [PubMed - as supplied by publisher]
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Evaluation of the Cepheid Respiratory Syncytial Virus and Influenza Virus A/B real-time PCR analyte specific reagent.
Sails AD, Saunders D, Airs S, Roberts D, Eltringham G, Magee JG. - Health Protection Agency Newcastle Laboratory, Institute of Pathology, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne, NE4 6BE, UK.
Two rapid real-time RT-PCR assays, specific for respiratory syncytial virus (RSV) and Influenza A and B, were evaluated for the detection of these viruses in clinical respiratory samples. The RSV assay was applied to 100 samples and the Influenza A and B assay applied to 96 samples all of which had been tested previously by an "in-house" multiplex real-time PCR assay. Forty three samples were negative for RSV by both methods and 56 samples were positive by both methods. One sample was negative by the new RSV assay although it was positive for RSV A by the "in-house" test. Thirty nine samples were negative for influenza virus by both methods and 55 samples were positive by both assays. Two samples were negative by the new influenza assay however they were positive by the "in-house" influenza assay. The new assays did not cross react with samples containing other viruses including parainfluenza 1, 2,& 4; human metapnuemovirus; coronavirus 229E, NL63, OC43; rhinovirus; adenovirus; bocavirus and had a specificity of 100% and a sensitivity of 98.2% for RSV and 96.5% for influenza respectively. The results of this study demonstrate that the new assays are specific and sensitive for the detection of RSV and influenza viruses in clinical samples.
PMID: 19651159 [PubMed - as supplied by publisher]
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