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J Clin Microbiol. 2008 Dec 24. [Epub ahead of print]
Simple, sensitive, and swift sequencing of complete Avian Influenza H5N1 genomes.
H?per D, Hoffmann B, Beer M. - Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth full-length sequence analysis.
For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from HPAIV H5N1 diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument.
The presented method seamlessly integrates high-throughput Roche/454 pyrosequencing into diagnostics without necessitating additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline.
PMID: 19109473 [PubMed - as supplied by publisher]
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Simple, sensitive, and swift sequencing of complete Avian Influenza H5N1 genomes.
H?per D, Hoffmann B, Beer M. - Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany.
The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth full-length sequence analysis.
For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from HPAIV H5N1 diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument.
The presented method seamlessly integrates high-throughput Roche/454 pyrosequencing into diagnostics without necessitating additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline.
PMID: 19109473 [PubMed - as supplied by publisher]
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