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By Maggie Fox, Health and Science Editor
WASHINGTON, May 29 (Reuters) -
A new device can quickly detect 92 different viruses, including several strains of the feared H5N1 avian flu virus or other emerging new infections, U.S. researchers reported on Tuesday.
They said their mass spectrometer device can also be used in big hospitals to watch for outbreaks of dangerous drug-resistant infections.
"Therefore, we can keep up with the virus, even if there is a new variant of H5N1 circulating that is different from last year's," Ranga Sampath, executive director of Ibis Biosciences Inc., told Reuters.
Carlsbad, Californina-based Ibis, a division of Isis Pharmaceuticals, <ISIS.O>, worked with U.S. military researchers to fine-tune their device and test it against a range of flu viruses.
Flu is difficult to monitor because spot tests cannot identify the precise strain. When testing people for the H5N1 flu virus, the World Health Organization uses specialized labs that can spend weeks first growing and then testing tiny samples of virus.
The new detector, called the T5000, works in just four hours to identify the precise strain, the company reported in the Public Library of Science.
Ibis researchers worked with teams at the Naval Medical Research Unit in Cairo, Egypt, U.S. Army Medical Research in Infectious Diseases at Ft. Detrick, Maryland, Johns Hopkins University in Baltimore and other groups to test the T5000.
"We detected and correctly identified 92 mammalian and avian influenza isolates, representing 30 different H and N types, including 29 avian H5N1 isolates," the company said.
The device was 97 percent accurate in identifying the flu strain infecting 656 people who gave specimens between 1999 and 2006, it said.
WHO and the U.S. Centers for Disease Control and Prevention have been calling with greater and greater urgency for new and better diagnostic tests for flu and other microbes.
full article:
references:
Jiang Y, Hall TA, Hofstadler SA, Naviaux RK. Mitochondrial DNA Mutation Detection by Electrospray Mass Spectrometry. Clin Chem. 2007 Feb;53(2):195-203. Epub 2006 Dec 7.
Ecker DJ, Drader JJ, Gutierrez J, Gutierrez A, Hannis JC, Schink A, Sampath R, Blyn LB, Eshoo MW, Hall TA, Tobarmosquera M, Jiang Y, Sannes-Lowery KA, Cummins LL, Libby B, Walcott DJ, Massire C, Ranken R, Manalili S, Ivy C, Melton R, Levene H, Harpin V, Li F, White N, Pear M, Ecker JA, Samant V, Knize D, Robbins D, Rudnick K, Hajjar F, Hofstadler SA. The Ibis T5000 Universal Biosensor: An Automated Platform for Pathogen Identification and Strain Typing. JALA. 2006 Dec; 6(11):341-351.
Ecker JA, Massire C, Hall TA, Ranken R, Pennella TT, Agasino Ivy C, Blyn LB, Hofstadler SA, Endy TP, Scott PT, Lindler L, Hamilton T, Gaddy C, Snow K, Pe M, Fishbain J, Craft D, Deye G, Riddell S, Milstrey E, Petruccelli B, Brisse S, Harpin V, Schink A, Ecker DJ, Sampath R, Eshoo MW. Identification of Acinetobacter species and genotyping of Acinetobacter
baumannii by multilocus PCR and mass spectrometry. J Clin Microbiol. 2006 Aug;44(8):2921-32.
Hujer KM, Hujer AM, Hulten EA, Bajaksouzian S, Adams JM, Donskey CJ, Ecker DJ, Massire C, Eshoo MW, Sampath R, Thomson JM, Rather PN, Craft DW, Fishbain JT, Ewell AJ, Jacobs MR, Paterson DL, Bonomo RA. Multi-drug Resistant Acinetobacter spp. Isolates from Military and Civilian Patients Treated at the Walter Reed Army Medical Center: Analysis of Antibiotic Resistance Genes. Antimicrob Agents Chemother. 2006 Dec;50(12):4114-23. Epub 2006 Sep 25.
Ecker JA, Massire C, Hall TA, Ranken R, Pennella TT, Agasino Ivy C, Blyn LB, Hofstadler SA, Endy TP, Scott PT, Lindler L, Hamilton T, Gaddy C, Snow K, Pe M, Fishbain J, Craft D, Deye G, Riddell S, Milstrey E, Petruccelli B, Brisse S, Harpin V, Schink A, Ecker DJ, Sampath R, Eshoo MW. Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry. J Clin Microbiol. 2006 Aug;44(8):2921-32.
Russell KL, Broderick MP, Franklin SE, Blyn LB, Freed NE, Moradi E, Ecker
DJ, Kammerer PE, Osuna MA, Kajon AE, Morn CB, Ryan MA. Transmission dynamics and prospective environmental sampling of adenovirus in a
military recruit setting. J Infect Dis. 2006 Oct 1;194(7):877-85. Epub 2006 Aug 25.
Hofstadler SA, Hari KL, Sampath R, Blyn LB, Eshoo MW, Ecker DJ, Detection of Microbial Agents Using Broad-range PRC with Detection by Mass Spectrometry: The TIGER Concept. Chapter in Encyclopedia of Rapid Microbiological Methods. 2006
Ecker DJ and Carroll KC. Investments in High-Payoff Technologies Could Reduce Toll of Infections. ASM News. 71(12): 576-581
Hofstadler SA, Drader JJ, Schink A. Selective Ion Filtering by Digital Thresholding: A Method To Unwind Complex ESI-Mass Spectra and Eliminate Signals from Low Molecular Weight Chemical Noise. Anal Chem. 78(2): 372-378. January 15, 2006
Hall TA, Budowle B, Jiang Y, Blyn L, Eshoo M, Sannes-Lowery KA, Sampath R, Drader JJ, Hannis JC, Harrell P, Samant V, White N, Ecker DJ, Hofstadler SA Base composition analysis of human mitochondrial DNA using electrospray ionization mass spectrometry: A novel tool for the identification and differentiation of humans.
Anal Biochem. 2005 Jul 26
Hofstadler S.A., Sampath, R., Blyn, L.B., Eshoo, M.W., Hall, T.A., Jiang, Y., Drader, J.J., Hannis, J.C., Sannes-Lowery, K.A., Cummins, L.L., Libby, B., Walcott, D.J., Schink, A., Massire, C., Ranken, R., Gutierrez, J., Manalili, S., Ivy, C., Melton, R., Levene, H., Barrett-Wilt, G., Li, F., Zapp, V., White, N., Samant, V., McNeil, J.A., Knize, D., Robbins, D., Rudnick, K., Desai, A., Moradi, E. and Ecker, D.J. (2005). TIGER: The Universal Biosensor. Int. J. Mass Spectrom. 242(1): 23-41
Ecker et al; Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance. (2005) Proc. Natl. Acad. Sci (USA): 102(22), 8012-17
Sampath R., S.A., Hofstadler S.A., Blyn, L.B., Eshoo, M.W., Hall, T.A., Massire, C., Levene, H.M., Hannis, J.C., Harrell, P.M., Neuman, B., Buchmeier, M.J., Jiang, Y., Ranken, R., Drader, J.J., Samant, V., Griffey, R.H., McNeil, J.A., Crooke, S.T. and Ecker, D.J. (2005). Rapid Identification of Emerging Pathogens: Coronavirus. Emerg Infect Dis. 11(3):
Ecker DE, Sampath R, Willett P, Wyatt JR, Samant V, Massire C, Hall TA, Hari KL, McNeil JA, Buchen-Osmond C, Budowle B. The Microbial Rosetta Stone Database: A compilation of global and emerging infectious microorganisms and bioterrorist threat agents. BMC Microbiology 2005, 5:19.
Ecker DJ, Sampath R, Willett P, Samant V, Massire C, Hall TA, Hari K,
McNeil JA, Buchen-Osmond C, Budowle B. The Microbial Rosetta Stone
Database: A common structure for microbial biosecurity threat agents.
J Forensic Sci. 2005 Nov;50(6):1380-5.
Van Ert M.N., Hofstadler, S.A., Jiang, Y., Busch, J.D., Wagner, D.M., Drader, J.J., Ecker, D.J., Hannis, J.C., Huynh, L.Y., Schupp, J.M., Simonson, T.S. and Keim, P. (2004). Mass spectrometry provides accurate characterization of two genetic marker types in Bacillus anthracis. Biotechniques. 37(4): 642-4, 646, 648.
Sampath, R. & Ecker, D. J. in Forum on Microbial Threats: Learning from SARS: Preparing for the Next Disease Outbreak -- Workshop Summary (ed. Knobler, S. E., Mahmoud, A., Lemon, S.) 181-185 (The National Academies Press, Washington, D.C., 2004).
Jiang, Y. & Hofstadler, S. A. A highly efficient and automated method of purifying and desalting PCR products for analysis by electrospray ionization mass spectrometry. Anal. Biochem. 316 , 50-57 (2003).
WASHINGTON, May 29 (Reuters) -
A new device can quickly detect 92 different viruses, including several strains of the feared H5N1 avian flu virus or other emerging new infections, U.S. researchers reported on Tuesday.
They said their mass spectrometer device can also be used in big hospitals to watch for outbreaks of dangerous drug-resistant infections.
"Therefore, we can keep up with the virus, even if there is a new variant of H5N1 circulating that is different from last year's," Ranga Sampath, executive director of Ibis Biosciences Inc., told Reuters.
Carlsbad, Californina-based Ibis, a division of Isis Pharmaceuticals, <ISIS.O>, worked with U.S. military researchers to fine-tune their device and test it against a range of flu viruses.
Flu is difficult to monitor because spot tests cannot identify the precise strain. When testing people for the H5N1 flu virus, the World Health Organization uses specialized labs that can spend weeks first growing and then testing tiny samples of virus.
The new detector, called the T5000, works in just four hours to identify the precise strain, the company reported in the Public Library of Science.
Ibis researchers worked with teams at the Naval Medical Research Unit in Cairo, Egypt, U.S. Army Medical Research in Infectious Diseases at Ft. Detrick, Maryland, Johns Hopkins University in Baltimore and other groups to test the T5000.
"We detected and correctly identified 92 mammalian and avian influenza isolates, representing 30 different H and N types, including 29 avian H5N1 isolates," the company said.
The device was 97 percent accurate in identifying the flu strain infecting 656 people who gave specimens between 1999 and 2006, it said.
WHO and the U.S. Centers for Disease Control and Prevention have been calling with greater and greater urgency for new and better diagnostic tests for flu and other microbes.
full article:
references:
Jiang Y, Hall TA, Hofstadler SA, Naviaux RK. Mitochondrial DNA Mutation Detection by Electrospray Mass Spectrometry. Clin Chem. 2007 Feb;53(2):195-203. Epub 2006 Dec 7.
Ecker DJ, Drader JJ, Gutierrez J, Gutierrez A, Hannis JC, Schink A, Sampath R, Blyn LB, Eshoo MW, Hall TA, Tobarmosquera M, Jiang Y, Sannes-Lowery KA, Cummins LL, Libby B, Walcott DJ, Massire C, Ranken R, Manalili S, Ivy C, Melton R, Levene H, Harpin V, Li F, White N, Pear M, Ecker JA, Samant V, Knize D, Robbins D, Rudnick K, Hajjar F, Hofstadler SA. The Ibis T5000 Universal Biosensor: An Automated Platform for Pathogen Identification and Strain Typing. JALA. 2006 Dec; 6(11):341-351.
Ecker JA, Massire C, Hall TA, Ranken R, Pennella TT, Agasino Ivy C, Blyn LB, Hofstadler SA, Endy TP, Scott PT, Lindler L, Hamilton T, Gaddy C, Snow K, Pe M, Fishbain J, Craft D, Deye G, Riddell S, Milstrey E, Petruccelli B, Brisse S, Harpin V, Schink A, Ecker DJ, Sampath R, Eshoo MW. Identification of Acinetobacter species and genotyping of Acinetobacter
baumannii by multilocus PCR and mass spectrometry. J Clin Microbiol. 2006 Aug;44(8):2921-32.
Hujer KM, Hujer AM, Hulten EA, Bajaksouzian S, Adams JM, Donskey CJ, Ecker DJ, Massire C, Eshoo MW, Sampath R, Thomson JM, Rather PN, Craft DW, Fishbain JT, Ewell AJ, Jacobs MR, Paterson DL, Bonomo RA. Multi-drug Resistant Acinetobacter spp. Isolates from Military and Civilian Patients Treated at the Walter Reed Army Medical Center: Analysis of Antibiotic Resistance Genes. Antimicrob Agents Chemother. 2006 Dec;50(12):4114-23. Epub 2006 Sep 25.
Ecker JA, Massire C, Hall TA, Ranken R, Pennella TT, Agasino Ivy C, Blyn LB, Hofstadler SA, Endy TP, Scott PT, Lindler L, Hamilton T, Gaddy C, Snow K, Pe M, Fishbain J, Craft D, Deye G, Riddell S, Milstrey E, Petruccelli B, Brisse S, Harpin V, Schink A, Ecker DJ, Sampath R, Eshoo MW. Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry. J Clin Microbiol. 2006 Aug;44(8):2921-32.
Russell KL, Broderick MP, Franklin SE, Blyn LB, Freed NE, Moradi E, Ecker
DJ, Kammerer PE, Osuna MA, Kajon AE, Morn CB, Ryan MA. Transmission dynamics and prospective environmental sampling of adenovirus in a
military recruit setting. J Infect Dis. 2006 Oct 1;194(7):877-85. Epub 2006 Aug 25.
Hofstadler SA, Hari KL, Sampath R, Blyn LB, Eshoo MW, Ecker DJ, Detection of Microbial Agents Using Broad-range PRC with Detection by Mass Spectrometry: The TIGER Concept. Chapter in Encyclopedia of Rapid Microbiological Methods. 2006
Ecker DJ and Carroll KC. Investments in High-Payoff Technologies Could Reduce Toll of Infections. ASM News. 71(12): 576-581
Hofstadler SA, Drader JJ, Schink A. Selective Ion Filtering by Digital Thresholding: A Method To Unwind Complex ESI-Mass Spectra and Eliminate Signals from Low Molecular Weight Chemical Noise. Anal Chem. 78(2): 372-378. January 15, 2006
Hall TA, Budowle B, Jiang Y, Blyn L, Eshoo M, Sannes-Lowery KA, Sampath R, Drader JJ, Hannis JC, Harrell P, Samant V, White N, Ecker DJ, Hofstadler SA Base composition analysis of human mitochondrial DNA using electrospray ionization mass spectrometry: A novel tool for the identification and differentiation of humans.
Anal Biochem. 2005 Jul 26
Hofstadler S.A., Sampath, R., Blyn, L.B., Eshoo, M.W., Hall, T.A., Jiang, Y., Drader, J.J., Hannis, J.C., Sannes-Lowery, K.A., Cummins, L.L., Libby, B., Walcott, D.J., Schink, A., Massire, C., Ranken, R., Gutierrez, J., Manalili, S., Ivy, C., Melton, R., Levene, H., Barrett-Wilt, G., Li, F., Zapp, V., White, N., Samant, V., McNeil, J.A., Knize, D., Robbins, D., Rudnick, K., Desai, A., Moradi, E. and Ecker, D.J. (2005). TIGER: The Universal Biosensor. Int. J. Mass Spectrom. 242(1): 23-41
Ecker et al; Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance. (2005) Proc. Natl. Acad. Sci (USA): 102(22), 8012-17
Sampath R., S.A., Hofstadler S.A., Blyn, L.B., Eshoo, M.W., Hall, T.A., Massire, C., Levene, H.M., Hannis, J.C., Harrell, P.M., Neuman, B., Buchmeier, M.J., Jiang, Y., Ranken, R., Drader, J.J., Samant, V., Griffey, R.H., McNeil, J.A., Crooke, S.T. and Ecker, D.J. (2005). Rapid Identification of Emerging Pathogens: Coronavirus. Emerg Infect Dis. 11(3):
Ecker DE, Sampath R, Willett P, Wyatt JR, Samant V, Massire C, Hall TA, Hari KL, McNeil JA, Buchen-Osmond C, Budowle B. The Microbial Rosetta Stone Database: A compilation of global and emerging infectious microorganisms and bioterrorist threat agents. BMC Microbiology 2005, 5:19.
Ecker DJ, Sampath R, Willett P, Samant V, Massire C, Hall TA, Hari K,
McNeil JA, Buchen-Osmond C, Budowle B. The Microbial Rosetta Stone
Database: A common structure for microbial biosecurity threat agents.
J Forensic Sci. 2005 Nov;50(6):1380-5.
Van Ert M.N., Hofstadler, S.A., Jiang, Y., Busch, J.D., Wagner, D.M., Drader, J.J., Ecker, D.J., Hannis, J.C., Huynh, L.Y., Schupp, J.M., Simonson, T.S. and Keim, P. (2004). Mass spectrometry provides accurate characterization of two genetic marker types in Bacillus anthracis. Biotechniques. 37(4): 642-4, 646, 648.
Sampath, R. & Ecker, D. J. in Forum on Microbial Threats: Learning from SARS: Preparing for the Next Disease Outbreak -- Workshop Summary (ed. Knobler, S. E., Mahmoud, A., Lemon, S.) 181-185 (The National Academies Press, Washington, D.C., 2004).
Jiang, Y. & Hofstadler, S. A. A highly efficient and automated method of purifying and desalting PCR products for analysis by electrospray ionization mass spectrometry. Anal. Biochem. 316 , 50-57 (2003).