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Loop-mediated Isothermal Amplification for Influenza A (H5N1) Virus
Shanthi Jayawardena,* Chung Y. Cheung,* Ian Barr,? Kwok H. Chan,* Honglin Chen,* Yi Guan,* J.S. Malik Peiris,* and Leo L.M. Poon*
*The University of Hong Kong, Hong Kong Special Administrative Region, People?s Republic of China;
We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
Shanthi Jayawardena,* Chung Y. Cheung,* Ian Barr,? Kwok H. Chan,* Honglin Chen,* Yi Guan,* J.S. Malik Peiris,* and Leo L.M. Poon*
*The University of Hong Kong, Hong Kong Special Administrative Region, People?s Republic of China;
We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.