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Biosens Bioelectron. 2014 Oct 22;65C:211-219. doi: 10.1016/j.bios.2014.10.036. [Epub ahead of print]
A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity.
Watanabe Y1, Ito T2, Ibrahim MS3, Arai Y4, Hotta K5, Phuong HV6, Hang NL6, Mai LQ6, Soda K7, Yamaoka M8, Poetranto ED9, Wulandari L9, Hiramatsu H10, Daidoji T11, Kubota-Koketsu R12, Sriwilaijaroen N13, Nakaya T11, Okuno Y12, Takahashi T14, Suzuki T14, Ito T7, Hotta H8, Yamashiro T5, Hayashi T2, Morita K15, Ikuta K4, Suzuki Y16.
Author information
Abstract
A switch of viral hemagglutinin receptor binding specificity from bird-type α2,3- to human-type α2,6-linked sialic acid is necessary for an avian influenza virus to become a pandemic virus. In this study, an easy-to-use strip test to detect receptor binding specificity of influenza virus was developed. A biotinylated anti-hemagglutinin antibody that bound a broad range of group 1 influenza A viruses and latex-conjugated α2,3 (blue) and α2,6 (red) sialylglycopolymers were used in an immunochromatographic strip test, with avidin and lectin immobilized on a nitrocellulose membrane at test and control lines, respectively. Accumulation of a sialylglycopolymer-virus-antibody complex at the test line was visualized by eye. The strip test could be completed in 30min and did not require special equipment or skills, thereby avoiding some disadvantages of current methods for analyzing receptor binding specificity of influenza virus. The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to α2,6 sialylglycans at viral titers >128 hemagglutination units. The strip test results were in agreement with those of ELISA virus binding assays, with correlations >0.95. In conclusion, the immunochromatographic strip test developed in this study should be useful for monitoring potential changes in the receptor binding specificity of group 1 influenza A viruses in the field.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Detection of receptor binding specificity; Group 1 influenza A virus; H5N1 avian influenza virus; Immunochromatographic strip test; Pandemic potential; Sialylglycopolymer
PMID:
25461160
[PubMed - as supplied by publisher]
A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity.
Watanabe Y1, Ito T2, Ibrahim MS3, Arai Y4, Hotta K5, Phuong HV6, Hang NL6, Mai LQ6, Soda K7, Yamaoka M8, Poetranto ED9, Wulandari L9, Hiramatsu H10, Daidoji T11, Kubota-Koketsu R12, Sriwilaijaroen N13, Nakaya T11, Okuno Y12, Takahashi T14, Suzuki T14, Ito T7, Hotta H8, Yamashiro T5, Hayashi T2, Morita K15, Ikuta K4, Suzuki Y16.
Author information
Abstract
A switch of viral hemagglutinin receptor binding specificity from bird-type α2,3- to human-type α2,6-linked sialic acid is necessary for an avian influenza virus to become a pandemic virus. In this study, an easy-to-use strip test to detect receptor binding specificity of influenza virus was developed. A biotinylated anti-hemagglutinin antibody that bound a broad range of group 1 influenza A viruses and latex-conjugated α2,3 (blue) and α2,6 (red) sialylglycopolymers were used in an immunochromatographic strip test, with avidin and lectin immobilized on a nitrocellulose membrane at test and control lines, respectively. Accumulation of a sialylglycopolymer-virus-antibody complex at the test line was visualized by eye. The strip test could be completed in 30min and did not require special equipment or skills, thereby avoiding some disadvantages of current methods for analyzing receptor binding specificity of influenza virus. The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to α2,6 sialylglycans at viral titers >128 hemagglutination units. The strip test results were in agreement with those of ELISA virus binding assays, with correlations >0.95. In conclusion, the immunochromatographic strip test developed in this study should be useful for monitoring potential changes in the receptor binding specificity of group 1 influenza A viruses in the field.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Detection of receptor binding specificity; Group 1 influenza A virus; H5N1 avian influenza virus; Immunochromatographic strip test; Pandemic potential; Sialylglycopolymer
PMID:
25461160
[PubMed - as supplied by publisher]
