Tweet
PLoS One. 2014 Dec 1;9(12):e113629. doi: 10.1371/journal.pone.0113629. eCollection 2014.
Reliability of pseudotyped influenza viral particles in neutralizing antibody detection.
Yang J1, Li W1, Long Y1, Song S1, Liu J1, Zhang X1, Wang X2, Jiang S1, Liao G1.
Author information
Abstract
BACKGROUND:
Current influenza control strategies require an active surveillance system. Pseudotyped viral particles (pp) together with the evaluation of pre-existing immunity in a population might satisfy this requirement. However, the reliability of using pp in neutralizing antibody (nAb) detection are undefined.
METHODOLOGY/PRINCIPAL FINDINGS:
Pseudotyped particles of A(H1N1)pmd09 (A/California/7/2009) and HPAI H5N1 (A/Anhui/1/2005), as well as their reassortants, were generated. The reliability of using these pp in nAb detection were compared concurrently with the corresponding viruses by a hemagglutination inhibition test, as well as ELISA-, cytopathic effect-, and fluorescence-based microneutralization assays. In the qualitative detection on nAbs, the pp and their corresponding viruses were in complete agreement, with an R2 value equal to or near 1 in two different populations. In the quantitative detection on nAbs, although the geometric mean titers (95% confidence interval) differed between the pp and viruses, no significant difference was observed. Furthermore, humoral immunity against the reassortants was evaluated; our results indicated strong consistency between the nAbs against reassortant pp and those against na?ve pp harboring the same hemagglutinin.
CONCLUSION/SIGNIFICANCE:
The pp displayed high reliability in influenza virus nAb detection. The use of reassortant pp is a safe and convenient strategy for characterizing emerging influenza viruses and surveying the disease burden.
PMID:
25436460
[PubMed - in process]
Free full text
Reliability of pseudotyped influenza viral particles in neutralizing antibody detection.
Yang J1, Li W1, Long Y1, Song S1, Liu J1, Zhang X1, Wang X2, Jiang S1, Liao G1.
Author information
Abstract
BACKGROUND:
Current influenza control strategies require an active surveillance system. Pseudotyped viral particles (pp) together with the evaluation of pre-existing immunity in a population might satisfy this requirement. However, the reliability of using pp in neutralizing antibody (nAb) detection are undefined.
METHODOLOGY/PRINCIPAL FINDINGS:
Pseudotyped particles of A(H1N1)pmd09 (A/California/7/2009) and HPAI H5N1 (A/Anhui/1/2005), as well as their reassortants, were generated. The reliability of using these pp in nAb detection were compared concurrently with the corresponding viruses by a hemagglutination inhibition test, as well as ELISA-, cytopathic effect-, and fluorescence-based microneutralization assays. In the qualitative detection on nAbs, the pp and their corresponding viruses were in complete agreement, with an R2 value equal to or near 1 in two different populations. In the quantitative detection on nAbs, although the geometric mean titers (95% confidence interval) differed between the pp and viruses, no significant difference was observed. Furthermore, humoral immunity against the reassortants was evaluated; our results indicated strong consistency between the nAbs against reassortant pp and those against na?ve pp harboring the same hemagglutinin.
CONCLUSION/SIGNIFICANCE:
The pp displayed high reliability in influenza virus nAb detection. The use of reassortant pp is a safe and convenient strategy for characterizing emerging influenza viruses and surveying the disease burden.
PMID:
25436460
[PubMed - in process]
Free full text
