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J Virol Methods. 2014 Sep 16. pii: S0166-0934(14)00345-0. doi: 10.1016/j.jviromet.2014.09.003. [Epub ahead of print]
An optimized enzyme-linked lectin assay to measure influenza A virus neuraminidase inhibition antibody titers in human sera.
Couzens L1, Gao J2, Westgeest K3, Sandbulte M4, Lugovtsev V5, Fouchier R6, Eichelberger M7.
Author information
Abstract
Antibodies to neuraminidase (NA), the second most abundant surface protein on influenza virus, contribute toward protection against influenza. The traditional thiobarbituric acid (TBA) method to quantify NA inhibiting antibodies is cumbersome and not suitable for routine serology. An enzyme-linked lectin assay (ELLA) described by Lambre et al. (1990) is a practical alternative method for measuring NA inhibition (NI) titers. This report describes optimization of the ELLA for measuring NI titers in human sera against influenza A viruses, using H6N1 and H6N2 viruses as antigens. The optimized ELLA is subtype-specific and reproducible. While the titers measured by ELLA are somewhat greater than those measured by a miniaturized TBA method, seroconversion rates are the same, suggesting similarity in assay sensitivity under these optimized conditions. The ELLA described in this report provides a practical format for routine evaluation of human antibody responses to NA.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Antibody; Enzyme-linked lectin assay; Influenza; Neuraminidase; Serology
PMID:
25233882
[PubMed - as supplied by publisher]
An optimized enzyme-linked lectin assay to measure influenza A virus neuraminidase inhibition antibody titers in human sera.
Couzens L1, Gao J2, Westgeest K3, Sandbulte M4, Lugovtsev V5, Fouchier R6, Eichelberger M7.
Author information
Abstract
Antibodies to neuraminidase (NA), the second most abundant surface protein on influenza virus, contribute toward protection against influenza. The traditional thiobarbituric acid (TBA) method to quantify NA inhibiting antibodies is cumbersome and not suitable for routine serology. An enzyme-linked lectin assay (ELLA) described by Lambre et al. (1990) is a practical alternative method for measuring NA inhibition (NI) titers. This report describes optimization of the ELLA for measuring NI titers in human sera against influenza A viruses, using H6N1 and H6N2 viruses as antigens. The optimized ELLA is subtype-specific and reproducible. While the titers measured by ELLA are somewhat greater than those measured by a miniaturized TBA method, seroconversion rates are the same, suggesting similarity in assay sensitivity under these optimized conditions. The ELLA described in this report provides a practical format for routine evaluation of human antibody responses to NA.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Antibody; Enzyme-linked lectin assay; Influenza; Neuraminidase; Serology
PMID:
25233882
[PubMed - as supplied by publisher]
