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J Virol Methods. 2014 Sep 10. pii: S0166-0934(14)00342-5. doi: 10.1016/j.jviromet.2014.08.025. [Epub ahead of print]
Multi-colored immunochromatography using nanobeads for rapid and sensitive typing of seasonal influenza viruses.
Sakurai A1, Takayama K2, Nomura N1, Yamamoto N3, Sakoda Y3, Kobayashi Y2, Kida H3, Shibasaki F4.
Author information
Abstract
Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza viruses because of its rapid turnaround and ease of use. Despite the usefulness of IC, the limit of detection of common IC kits is as high as 103-104 plaque forming units (pfu) per reaction, resulting in their limited sensitivities. Early diagnosis within 24h would provide more appropriate timing of treatment. In this study, a multi-colored NanoAct? bead IC was established to detect seasonal influenza viruses. This method has approximately 10-fold higher sensitivity than that of colloidal gold or colored latex bead IC assays, and does not require specific instruments. More notably, NanoAct? bead IC can distinguish influenza A and B viruses from clinical samples with a straightforward readout composed of colored lines. Our results will provide new strategies for the diagnosis, treatment, and a chance to survey of influenza viruses in developing countries and in the field research.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Diagnosis; Immunochromatography; Influenza viruses; Nanobead
PMID:
25218175
[PubMed - as supplied by publisher]
Multi-colored immunochromatography using nanobeads for rapid and sensitive typing of seasonal influenza viruses.
Sakurai A1, Takayama K2, Nomura N1, Yamamoto N3, Sakoda Y3, Kobayashi Y2, Kida H3, Shibasaki F4.
Author information
Abstract
Immunochromatography (IC) is an antigen-detection assay that plays an important role in the rapid diagnosis of influenza viruses because of its rapid turnaround and ease of use. Despite the usefulness of IC, the limit of detection of common IC kits is as high as 103-104 plaque forming units (pfu) per reaction, resulting in their limited sensitivities. Early diagnosis within 24h would provide more appropriate timing of treatment. In this study, a multi-colored NanoAct? bead IC was established to detect seasonal influenza viruses. This method has approximately 10-fold higher sensitivity than that of colloidal gold or colored latex bead IC assays, and does not require specific instruments. More notably, NanoAct? bead IC can distinguish influenza A and B viruses from clinical samples with a straightforward readout composed of colored lines. Our results will provide new strategies for the diagnosis, treatment, and a chance to survey of influenza viruses in developing countries and in the field research.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Diagnosis; Immunochromatography; Influenza viruses; Nanobead
PMID:
25218175
[PubMed - as supplied by publisher]
