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Biosens Bioelectron. 2012 Nov 6;42C:236-241. doi: 10.1016/j.bios.2012.10.067. [Epub ahead of print]
Colorimetric viral detection based on sialic acid stabilized gold nanoparticles.
Lee C, Gaston MA, Weiss AA, Zhang P.
Source
Department of Chemistry, Biochemistry & Microbiology, University of Cincinnati, Cincinnati, OH 45221, United States.
Abstract
Sialic acid reduced and stabilized gold nanoparticles (d=20.1?1.8nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid for colorimetric detection of influenza virus. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutinin-sialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B/Victoria and influenza B/Yamagata. Virus dilution (hemagglutination assay titer, 512) of 0.156vol% was readily detected. The upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles.
Copyright ? 2012 Elsevier B.V. All rights reserved.
PMID:
23208092
[PubMed - as supplied by publisher]
Colorimetric viral detection based on sialic acid stabilized gold nanoparticles.
Lee C, Gaston MA, Weiss AA, Zhang P.
Source
Department of Chemistry, Biochemistry & Microbiology, University of Cincinnati, Cincinnati, OH 45221, United States.
Abstract
Sialic acid reduced and stabilized gold nanoparticles (d=20.1?1.8nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid for colorimetric detection of influenza virus. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutinin-sialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B/Victoria and influenza B/Yamagata. Virus dilution (hemagglutination assay titer, 512) of 0.156vol% was readily detected. The upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles.
Copyright ? 2012 Elsevier B.V. All rights reserved.
PMID:
23208092
[PubMed - as supplied by publisher]
