Comparison of commercial enzyme-linked immunosorbent assay kits with agar gel precipitation and hemagglutination-inhibition tests for detecting antibodies to avian influenza viruses

[tetano]

J Vet Med Sci. 2012 Oct 1;74(9):1217-20. Epub 2012 May 16.
Comparison of commercial enzyme-linked immunosorbent assay kits with agar gel precipitation and hemagglutination-inhibition tests for detecting antibodies to avian influenza viruses.
Shiraishi R, Nishiguchi A, Tsukamoto K, Muramatsu M.
Source

Research Institute for Animal Science in Biochemistry & Toxicology, 3-7-11 Hashimotodai, Midori-ku, Sagamihara, Kanagawa 252-0132, Japan.
Abstract

We evaluated the utility of 5 commercial enzyme-linked immunosorbent assay (ELISA) kits for detecting antibodies to avian influenza viruses. The sensitivities and specificities of the ELISA kits were compared with those of the agar gel precipitation (AGP) and hemagglutination-inhibition (HI) tests. The results suggest that some ELISA kits might not be suitable for monitoring during the early stages of avian influenza virus infections. Therefore, ELISA kits should only be used in conjunction with a profound knowledge about monitoring of avian influenza.

PMID:
23037780
[PubMed - in process]

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http://www.ncbi.nlm.nih.gov/pubmed/23037780