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Host Gene Expression Signatures Discriminate between Ferrets Infected with Genetically Similar H1N1 Strains

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  • Host Gene Expression Signatures Discriminate between Ferrets Infected with Genetically Similar H1N1 Strains

    PLoS One. 2012;7(7):e40743. Epub 2012 Jul 13.
    Host Gene Expression Signatures Discriminate between Ferrets Infected with Genetically Similar H1N1 Strains.
    Ljungberg K, McBrayer A, Camp JV, Chu YK, Tapp R, Noah DL, Grimes S, Proctor ML, Liljestr?m P, Jonsson CB, Bruder CE.
    Source

    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
    Abstract

    Different respiratory viruses induce virus-specific gene expression in the host. Recent evidence, including those presented here, suggests that genetically related isolates of influenza virus induce strain-specific host gene regulation in several animal models. Here, we identified systemic strain-specific gene expression signatures in ferrets infected with pandemic influenza A/California/07/2009, A/Mexico/4482/2009 or seasonal influenza A/Brisbane/59/2007. Using uncorrelated shrunken centroid classification, we were able to accurately identify the infecting influenza strain with a combined gene expression profile of 10 selected genes, independent of the severity of disease. Another gene signature, consisting of 7 genes, could classify samples based on lung pathology. Furthermore, we identified a gene expression profile consisting of 31 probes that could classify samples based on both strain and severity of disease. Thus, we show that expression-based analysis of non-infected tissue enables distinction between genetically related influenza viruses as well as lung pathology. These results open for development of alternative tools for influenza diagnostics.

    PMID:
    22808249
    [PubMed - in process]
    PMCID:
    PMC3396591

    Free full text

    Different respiratory viruses induce virus-specific gene expression in the host. Recent evidence, including those presented here, suggests that genetically related isolates of influenza virus induce strain-specific host gene regulation in several animal models. Here, we identified systemic strain-sp …
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