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Evaluation of the Xpert Flu test and comparison with in-house real-time RT-PCR assays for detection of influenza virus from 2008 to 2011 in Marseille, France

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  • Evaluation of the Xpert Flu test and comparison with in-house real-time RT-PCR assays for detection of influenza virus from 2008 to 2011 in Marseille, France

    Clin Microbiol Infect. 2012 Feb 20. doi: 10.1111/j.1469-0691.2012.03792.x. [Epub ahead of print]
    Evaluation of the Xpert Flu test and comparison with in-house real-time RT-PCR assays for detection of influenza virus from 2008 to 2011 in Marseille, France.
    Salez N, Ninove L, Thirion L, Gazin C, Zandotti C, de Lamballerie X, Charrel RN.
    Source

    ) UMR190 'Emergence des Pathologies Virales'(Aix-Marseille Univ - Institute of Research for Development - EHESP French School of Public Health), Marseille, France ) Federation of Clinical Microbiology, AP-HM Timone, Marseille, France.
    Abstract

    Clin Microbiol Infect ABSTRACT: Rapid documentation of respiratory specimens can have an impact on the management of patients and their relatives in terms of preventive and curative measures. We compared the results of the Xpert() Flu assay (Cepheid) with three real-time RT-PCR assays using 127 nasopharyngeal samples, of which 75 were positive for influenza A (with 52 identified as A/H1N1-2009) and 52 were positive for influenza B. The Xpert() Flu assay presented a quasi-absence of non-interpretable tests, and showed sensitivity and specificity of 100% and 100% for Flu A, 98.4% and 100% for A/H1N1-2009, and 80.7% and 100% for Flu B.

    2012 The Authors Clinical Microbiology and Infection 2012 European Society of Clinical Microbiology and Infectious Diseases.

    PMID:
    22360446
    [PubMed - as supplied by publisher]

    http://www.ncbi.nlm.nih.gov/pubmed/22360446
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