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Direct Fluorescent Antibody Followed by Culture for Diagnosis of 2009 H1N1 Influenza A

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  • Direct Fluorescent Antibody Followed by Culture for Diagnosis of 2009 H1N1 Influenza A

    J Clin Microbiol. 2011 Aug 17. [Epub ahead of print]
    Direct Fluorescent Antibody Followed by Culture for Diagnosis of 2009 H1N1 Influenza A.
    Bakerman P, Balasuriya L, Fried O, Tellez D, Garcia-Filion P, Dalton H.
    Source

    Phoenix Children's Hospital, Pediatric Intensive Care Unit.
    Abstract

    During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent antibody with polymerase chain reaction confirmation. Direct fluorescent antibody has specificity of 99.6%, but sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent antibody which missed one third of infected individuals.

    PMID:
    21849690
    [PubMed - as supplied by publisher]

    During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct …
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