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Biosens Bioelectron
. 2025 Jul 31:117829.
doi: 10.1016/j.bios.2025.117829. Online ahead of print. Highly sensitive reusable electrochemical sensor for H1N1 influenza A virus nucleoproteins
Sang Hyeon Park 1 , Seok Cheol Kim 1 , Aejin Lee 1 , Jong Kwan Park 1 , Eun Bin Kang 1 , Seul Bin Ha 1 , Pan Kee Bae 2 , Jung Sun Kwon 2 , Wan Soo Yun 3
Affiliations
There is growing interest in reusable biosensors that can detect pathogens, particularly for disease diagnosis and environmental monitoring. It is difficult to implement a reusable biosensor for large biomolecules such as proteins because target molecules must be removed from sensor-surface-immobilized receptors without deteriorating their activity to restore the sensor to its initial state. While a high binding affinity generally improves the limit of detection (LOD), it also hinders recovery. In other words, the reusability and performance of a biosensor tend to be inversely proportional. To overcome this trade-off, we propose a combination of microbeads functionalized with enzyme-linked receptors and a solid-state electrochemical sensor that detects the signal of the electrochemical probe generated by the enzyme in a solution-phase reaction. In this study, 50 measurements of H1N1 nucleoprotein were achieved without affecting the femtomolar LOD of the biosensor.
Keywords: Enzymatic amplification; Influenza A virus subtype H1N1; Microgap sensor; Reusable sensor.
. 2025 Jul 31:117829.
doi: 10.1016/j.bios.2025.117829. Online ahead of print. Highly sensitive reusable electrochemical sensor for H1N1 influenza A virus nucleoproteins
Sang Hyeon Park 1 , Seok Cheol Kim 1 , Aejin Lee 1 , Jong Kwan Park 1 , Eun Bin Kang 1 , Seul Bin Ha 1 , Pan Kee Bae 2 , Jung Sun Kwon 2 , Wan Soo Yun 3
Affiliations
- PMID: 40750471
- DOI: 10.1016/j.bios.2025.117829
There is growing interest in reusable biosensors that can detect pathogens, particularly for disease diagnosis and environmental monitoring. It is difficult to implement a reusable biosensor for large biomolecules such as proteins because target molecules must be removed from sensor-surface-immobilized receptors without deteriorating their activity to restore the sensor to its initial state. While a high binding affinity generally improves the limit of detection (LOD), it also hinders recovery. In other words, the reusability and performance of a biosensor tend to be inversely proportional. To overcome this trade-off, we propose a combination of microbeads functionalized with enzyme-linked receptors and a solid-state electrochemical sensor that detects the signal of the electrochemical probe generated by the enzyme in a solution-phase reaction. In this study, 50 measurements of H1N1 nucleoprotein were achieved without affecting the femtomolar LOD of the biosensor.
Keywords: Enzymatic amplification; Influenza A virus subtype H1N1; Microgap sensor; Reusable sensor.