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Virology
. 2025 Jun 18:610:110609.
doi: 10.1016/j.virol.2025.110609. Online ahead of print. PathoSift Pro: A novel size-based bioaerosol sampler for effectively detecting infectious airborne influenza virus
Xuan-Dung Nguyen 1 , Bac Le 1 , Qiongying Yang 1 , Binbin Wang 2 , Xiu-Feng Wan 3
Affiliations
Understanding the dynamics of airborne virus transmission requires precise tools for collecting and analyzing infectious aerosols. We present PathoSift Pro, a novel bioaerosol sampler engineered to efficiently collect and preserve viable airborne pathogens. Operating at a flow rate of 25 L/min, PathoSift Pro minimizes dehydration and re-aerosolization, overcoming the limitations of conventional samplers like the Andersen impactor. Using H1N1 influenza A virus as a model, our study demonstrated that PathoSift Pro effectively captured size distributions of virus-laden particles in simulated settings and maintained 74.76 % more viable viruses than the Andersen impactor. To further evaluate its field applicability, we tested PathoSift Pro through experiments using a pig model. Results showed that pigs exhibited aerosol size distributions similar to humans, with viable viruses detected in both aerosol (≤5 μm) and droplet (>5 μm) particles, supporting pigs as a translational model for influenza transmission studies. In summary, PathoSift Pro provides an effective tool for advancing research on airborne pathogens and elucidating airborne transmission dynamics.
Keywords: Airborne influenza virus transmission; Bioaerosol sampling; Dehydration effects; Particle size distribution; PathoSift pro; Pig model; Re-aerosolization; Viable virus detection.
. 2025 Jun 18:610:110609.
doi: 10.1016/j.virol.2025.110609. Online ahead of print. PathoSift Pro: A novel size-based bioaerosol sampler for effectively detecting infectious airborne influenza virus
Xuan-Dung Nguyen 1 , Bac Le 1 , Qiongying Yang 1 , Binbin Wang 2 , Xiu-Feng Wan 3
Affiliations
- PMID: 40561866
- DOI: 10.1016/j.virol.2025.110609
Understanding the dynamics of airborne virus transmission requires precise tools for collecting and analyzing infectious aerosols. We present PathoSift Pro, a novel bioaerosol sampler engineered to efficiently collect and preserve viable airborne pathogens. Operating at a flow rate of 25 L/min, PathoSift Pro minimizes dehydration and re-aerosolization, overcoming the limitations of conventional samplers like the Andersen impactor. Using H1N1 influenza A virus as a model, our study demonstrated that PathoSift Pro effectively captured size distributions of virus-laden particles in simulated settings and maintained 74.76 % more viable viruses than the Andersen impactor. To further evaluate its field applicability, we tested PathoSift Pro through experiments using a pig model. Results showed that pigs exhibited aerosol size distributions similar to humans, with viable viruses detected in both aerosol (≤5 μm) and droplet (>5 μm) particles, supporting pigs as a translational model for influenza transmission studies. In summary, PathoSift Pro provides an effective tool for advancing research on airborne pathogens and elucidating airborne transmission dynamics.
Keywords: Airborne influenza virus transmission; Bioaerosol sampling; Dehydration effects; Particle size distribution; PathoSift pro; Pig model; Re-aerosolization; Viable virus detection.