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Viral Immunol. 2018 Nov 22. doi: 10.1089/vim.2018.0120. [Epub ahead of print]
Association of Single Nucleotide Polymorphisms in TNFA and IL10 Genes with Disease Severity in Influenza A/H1N1pdm09 Virus Infections: A Study from Western India.
Choudhary ML1, Alagarasu K1, Choudhary U1, Kawale S1, Malasane P1, Gurav YK1, Padbidri V2, Kadam D3, Sangle SA3, Salvi S3, Bavdekar AR4, D'costa P4, Chadha MS1.
Author information
Abstract
Susceptibility to severe influenza A/H1N1pdm09 virus is multifactorial. The present study was carried out in 246 patients infected with A/H1N1pdm09 virus to find out whether single nucleotide polymorphisms (SNPs) in the genes coding for proinflammatory and anti-inflammatory cytokines are associated with disease severity. Among the cases, 129 had mild disease, whereas 117 had severe disease. There were 27 fatal cases. TNFA rs1800629, IFNG rs2430561, IL10 rs1800872, IL10 rs1800896, and CCL2 rs1024611 SNPs were genotyped by polymerase chain reaction-based methods. A significantly higher frequency of TNFA rs1800629 "G/A" genotype was observed in severe and fatal cases compared with mild and survived cases, respectively. In a dominant mode, IL10 rs1800896 "G" allele was significantly negatively associated with disease severity. IL10 rs1800896 "C/A" genotype was significantly associated with fatality in influenza A/H1N1pdm09 infections. The results suggest that SNPs in the IL10 and TNFA genes might be associated with disease severity in influenza A/H1N1pdm09-infected patients.
KEYWORDS:
cytokines; disease severity; gene polymorphisms; influenza A/H1N1pdm09 virus; interferon-γ; interleukin-10; monocyte chemoattractant protein-1; tumor necrosis factor-α
PMID: 30475169 DOI: 10.1089/vim.2018.0120
Association of Single Nucleotide Polymorphisms in TNFA and IL10 Genes with Disease Severity in Influenza A/H1N1pdm09 Virus Infections: A Study from Western India.
Choudhary ML1, Alagarasu K1, Choudhary U1, Kawale S1, Malasane P1, Gurav YK1, Padbidri V2, Kadam D3, Sangle SA3, Salvi S3, Bavdekar AR4, D'costa P4, Chadha MS1.
Author information
Abstract
Susceptibility to severe influenza A/H1N1pdm09 virus is multifactorial. The present study was carried out in 246 patients infected with A/H1N1pdm09 virus to find out whether single nucleotide polymorphisms (SNPs) in the genes coding for proinflammatory and anti-inflammatory cytokines are associated with disease severity. Among the cases, 129 had mild disease, whereas 117 had severe disease. There were 27 fatal cases. TNFA rs1800629, IFNG rs2430561, IL10 rs1800872, IL10 rs1800896, and CCL2 rs1024611 SNPs were genotyped by polymerase chain reaction-based methods. A significantly higher frequency of TNFA rs1800629 "G/A" genotype was observed in severe and fatal cases compared with mild and survived cases, respectively. In a dominant mode, IL10 rs1800896 "G" allele was significantly negatively associated with disease severity. IL10 rs1800896 "C/A" genotype was significantly associated with fatality in influenza A/H1N1pdm09 infections. The results suggest that SNPs in the IL10 and TNFA genes might be associated with disease severity in influenza A/H1N1pdm09-infected patients.
KEYWORDS:
cytokines; disease severity; gene polymorphisms; influenza A/H1N1pdm09 virus; interferon-γ; interleukin-10; monocyte chemoattractant protein-1; tumor necrosis factor-α
PMID: 30475169 DOI: 10.1089/vim.2018.0120