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J Infect Dis. 2018 Jan 12. doi: 10.1093/infdis/jiy013. [Epub ahead of print]
Kinetics of serological responses in critically ill patients hospitalized with influenza A(H1N1)pdm09 virus infection in Canada, 2009-2011.
Rolfes MA1, Gross FL1,2, Flannery B1, Meyers AFA3,4,5, Luo M3, Bastien N3, Fowler RA6, Katz JM1, Levine MZ1, Kumar A7, Uyeki TM1; Canadian Severe Influenza Study (CSIS) and the Randomized, Double-Blinded Controlled Trial Comparing High vs Standard Dose Oseltamivir in Severe, Influenza Infection in ICU (ROSII) Study Groups.
Author information
Abstract
Background:
The kinetics of the antibody response during severe influenza are not well documented.
Methods:
Critically ill patients with influenza A(H1N1)pdm09 virus infection, confirmed by RT-PCR or seroconversion (≥4-fold rise in titers), during 2009-2011 in Canada were prospectively studied. Antibody titers in serially collected sera were determined using hemagglutinin inhibition (HAI) and microneutralization assays. Average antibody curves were estimated using linear mixed effects models and compared by patient outcome, age, and corticosteroid treatment.
Results:
Of 47 patients with A(H1N1)pdm09 virus infection (median age 47 years), 59% had baseline HAI and 68% had baseline neutralizing titers <40. Antibody titers rose quickly after symptom onset and, by day 14, 83% of patients had HAI and 80% had neutralizing titers ≥40. Baseline HAI titers were significantly higher in patients who died compared with patients who survived; however, the antibody kinetics were similar by patient outcome and corticosteroid treatment. Geometric mean titers over time in older patients were lower compared with younger patients.
Conclusions:
Critically ill patients with influenza A(H1N1)pdm09 virus infection had strong HAI and neutralizing antibody responses during their illness. Antibody kinetics differed by age but were not associated with patient outcome.
KEYWORDS:
Influenza; critical illness; humoral immunity
PMID: 29342251 DOI: 10.1093/infdis/jiy013
Kinetics of serological responses in critically ill patients hospitalized with influenza A(H1N1)pdm09 virus infection in Canada, 2009-2011.
Rolfes MA1, Gross FL1,2, Flannery B1, Meyers AFA3,4,5, Luo M3, Bastien N3, Fowler RA6, Katz JM1, Levine MZ1, Kumar A7, Uyeki TM1; Canadian Severe Influenza Study (CSIS) and the Randomized, Double-Blinded Controlled Trial Comparing High vs Standard Dose Oseltamivir in Severe, Influenza Infection in ICU (ROSII) Study Groups.
Author information
Abstract
Background:
The kinetics of the antibody response during severe influenza are not well documented.
Methods:
Critically ill patients with influenza A(H1N1)pdm09 virus infection, confirmed by RT-PCR or seroconversion (≥4-fold rise in titers), during 2009-2011 in Canada were prospectively studied. Antibody titers in serially collected sera were determined using hemagglutinin inhibition (HAI) and microneutralization assays. Average antibody curves were estimated using linear mixed effects models and compared by patient outcome, age, and corticosteroid treatment.
Results:
Of 47 patients with A(H1N1)pdm09 virus infection (median age 47 years), 59% had baseline HAI and 68% had baseline neutralizing titers <40. Antibody titers rose quickly after symptom onset and, by day 14, 83% of patients had HAI and 80% had neutralizing titers ≥40. Baseline HAI titers were significantly higher in patients who died compared with patients who survived; however, the antibody kinetics were similar by patient outcome and corticosteroid treatment. Geometric mean titers over time in older patients were lower compared with younger patients.
Conclusions:
Critically ill patients with influenza A(H1N1)pdm09 virus infection had strong HAI and neutralizing antibody responses during their illness. Antibody kinetics differed by age but were not associated with patient outcome.
KEYWORDS:
Influenza; critical illness; humoral immunity
PMID: 29342251 DOI: 10.1093/infdis/jiy013