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J Gen Virol. 2016 Jul 13. doi: 10.1099/jgv.0.000552. [Epub ahead of print]
Influenzavirus A(H1N1)2009 antibody-dependent cellular cytotoxicity in young children prior to the H1N1 pandemic.
Mesman AW1, Westerhuis BM2, Ten Hulscher HI3, Jacobi RH4, de Bruin E5, van Beek J6, Buisman AM7, Koopmans MP8, van Binnendijk RS9.
Author information
Abstract
Pre-existing immunity has played a significant role in protection during the latest influenza A virus H1N1 pandemic, especially in older age groups. Structural similarities were found between A(H1N1)2009 and older H1N1 virus strains to which humans have already been exposed in the past. Broadly cross-reactive antibodies capable of neutralizing A(H1N1)2009 virus have been implicated in this immune protection in adults. Here we investigated the serological profile of a group of young children 9 years of age (n=55), from which paired blood samples were available just prior to the pandemic wave (March 2009) and shortly thereafter (March 2010). On the basis of A(H1N1)2009 seroconversion, 27 of 55 children (49%) were confirmed to be infected between these 2 time points. Within the non-infected group of 28 children (51%), high levels of seasonal antibodies to H1 and H3 HA1 antigens were detected prior to pandemic exposure, reflecting past infection with H1N1 and H3N2, both of which had circulated in The Netherlands prior to the pandemic. In some children, this reactivity coincided with specific antibody reactivity against A(H1N1)2009. While these antibodies were not able to neutralize A(H1N1)2009 virus, they were able to mediate antibody-dependent cellular cytotoxicity (ADCC) in vitro upon interaction with A(H1N1)2009 virus. This suggests that cross-reactive antibodies could contribute to immune protection in children via ADCC.
PMID: 27412007 DOI: 10.1099/jgv.0.000552
[PubMed - as supplied by publisher]
Influenzavirus A(H1N1)2009 antibody-dependent cellular cytotoxicity in young children prior to the H1N1 pandemic.
Mesman AW1, Westerhuis BM2, Ten Hulscher HI3, Jacobi RH4, de Bruin E5, van Beek J6, Buisman AM7, Koopmans MP8, van Binnendijk RS9.
Author information
Abstract
Pre-existing immunity has played a significant role in protection during the latest influenza A virus H1N1 pandemic, especially in older age groups. Structural similarities were found between A(H1N1)2009 and older H1N1 virus strains to which humans have already been exposed in the past. Broadly cross-reactive antibodies capable of neutralizing A(H1N1)2009 virus have been implicated in this immune protection in adults. Here we investigated the serological profile of a group of young children 9 years of age (n=55), from which paired blood samples were available just prior to the pandemic wave (March 2009) and shortly thereafter (March 2010). On the basis of A(H1N1)2009 seroconversion, 27 of 55 children (49%) were confirmed to be infected between these 2 time points. Within the non-infected group of 28 children (51%), high levels of seasonal antibodies to H1 and H3 HA1 antigens were detected prior to pandemic exposure, reflecting past infection with H1N1 and H3N2, both of which had circulated in The Netherlands prior to the pandemic. In some children, this reactivity coincided with specific antibody reactivity against A(H1N1)2009. While these antibodies were not able to neutralize A(H1N1)2009 virus, they were able to mediate antibody-dependent cellular cytotoxicity (ADCC) in vitro upon interaction with A(H1N1)2009 virus. This suggests that cross-reactive antibodies could contribute to immune protection in children via ADCC.
PMID: 27412007 DOI: 10.1099/jgv.0.000552
[PubMed - as supplied by publisher]