Tweet
J Infect Dis. Plasmacytoid Dendritic Cells and CD8 T cells from Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
[Source: The Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
Plasmacytoid Dendritic Cells and CD8 T cells from Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
Rebecca L. Vanders 1,2, Peter G. Gibson 1,3, Vanessa E. Murphy 1,2 and Peter A. B. Wark 1,3
Author Affiliations: <SUP>1</SUP>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle, Newcastle, Australia, 2308 <SUP>2</SUP>Hunter Medical Research Institute, Newcastle, Australia, 2305 <SUP>3</SUP>Department of Respiratory and Sleep Medicine, John Hunter Hospital, Newcastle, Australia, 2305
Corresponding Author: Dr. Rebecca Vanders, Centre for Asthma and Respiratory Diseases, Level 2, West Wing, Hunter Medical Research Institute, University of Newcastle, University Drive, Callaghan NSW 2308. Phone: 61 2 40420107 Fax: 61 2 40420046, email: Rebecca.Vanders@newcastle.edu.au
Abstract
Background.
Pregnant women are a high risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09).
Methods.
Peripheral blood mononuclear cells (PBMCs) were isolated from n=26 pregnant and n=28 non-pregnant women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, HLA-DR, and Programmed Death Ligand 1/2 (PDL1/2) measured on pDCs. Programmed Death receptor 1 (PD1) was measured on CD8 T cells. IFNα, IL2, TNFα and IFNγ were measured from culture supernatant.
Results.
pDC (i.e.CD303<SUP>+</SUP>/CD1c<SUP>−</SUP>PBMCs) percentages were lower in pregnant compared to non-pregnant women (p<0.05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (p<0.01), HLA-DR (p<0.001) and PDL1 (p<0.001), compared to non-pregnant women. Expression of PD1 on CD8 T cells was also higher during pregnancy (p<0.05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFNα (p<0.01), IL2 (p<0.01) and IFNγ (p<0.01) compared to non-pregnant women, however blocking PDL1 during H1N1/09 infection increased these cytokines from PBMCs of pregnant women (p<0.05).
Conclusion.
Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
Received January 15, 2013. Revision received March 12, 2013. Accepted March 20, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
-
------
Plasmacytoid Dendritic Cells and CD8 T cells from Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection
Rebecca L. Vanders 1,2, Peter G. Gibson 1,3, Vanessa E. Murphy 1,2 and Peter A. B. Wark 1,3
Author Affiliations: <SUP>1</SUP>Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle, Newcastle, Australia, 2308 <SUP>2</SUP>Hunter Medical Research Institute, Newcastle, Australia, 2305 <SUP>3</SUP>Department of Respiratory and Sleep Medicine, John Hunter Hospital, Newcastle, Australia, 2305
Corresponding Author: Dr. Rebecca Vanders, Centre for Asthma and Respiratory Diseases, Level 2, West Wing, Hunter Medical Research Institute, University of Newcastle, University Drive, Callaghan NSW 2308. Phone: 61 2 40420107 Fax: 61 2 40420046, email: Rebecca.Vanders@newcastle.edu.au
Abstract
Background.
Pregnant women are a high risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09).
Methods.
Peripheral blood mononuclear cells (PBMCs) were isolated from n=26 pregnant and n=28 non-pregnant women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, HLA-DR, and Programmed Death Ligand 1/2 (PDL1/2) measured on pDCs. Programmed Death receptor 1 (PD1) was measured on CD8 T cells. IFNα, IL2, TNFα and IFNγ were measured from culture supernatant.
Results.
pDC (i.e.CD303<SUP>+</SUP>/CD1c<SUP>−</SUP>PBMCs) percentages were lower in pregnant compared to non-pregnant women (p<0.05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (p<0.01), HLA-DR (p<0.001) and PDL1 (p<0.001), compared to non-pregnant women. Expression of PD1 on CD8 T cells was also higher during pregnancy (p<0.05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFNα (p<0.01), IL2 (p<0.01) and IFNγ (p<0.01) compared to non-pregnant women, however blocking PDL1 during H1N1/09 infection increased these cytokines from PBMCs of pregnant women (p<0.05).
Conclusion.
Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.
Received January 15, 2013. Revision received March 12, 2013. Accepted March 20, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
-
------