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Efficient inhibition of influenza A viral replication in cells by deoxyribozymes delivered by nanocomposites

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  • Efficient inhibition of influenza A viral replication in cells by deoxyribozymes delivered by nanocomposites

    Int J Antimicrob Agents. 2017 Apr 12. pii: S0924-8579(17)30113-9. doi: 10.1016/j.ijantimicag.2017.01.026. [Epub ahead of print]
    Efficient inhibition of influenza A viral replication in cells by deoxyribozymes delivered by nanocomposites.

    Repkova M1, Levina A1, Chelobanov B2, Ismagilov Z3, Shatskaya N4, Baiborodin S4, Filippova E5, Mazurkova N5, Zarytova V6.
    Author information

    Abstract

    Nucleic-acid-based drugs are a promising class of novel therapeutics; however, their use in medicine is widely limited because of insufficient delivery into cells. This article proposes a new delivery strategy of nucleic acid fragments into cells as components of TiO2-based nanocomposites. For the first time, unmodified Dz molecules were non-covalently immobilized on TiO2 nanoparticles precovered with polylysine (TiO2?PL) with the formation of (TiO2?PL)?Dz nanocomposites. DNAzymes in the proposed nanocomposites were shown to retain their ability to cleave the RNA target in a cell-free system with the same selectivity as unbound Dz molecules. It was shown by confocal laser microscopy that the fluorescein-labelled (TiO2?PL)?DzFlu nanocomposites penetrate into eukaryotic cells, where DzFlu is internalized in the cytoplasm and predominantly in nuclei. Delivery of deoxyribozymes into cells in the proposed nanocomposites permits very efficient interactions with RNA targets inside cells. This was demonstrated by an example of inhibition of H5N1 influenza A virus replication (inhibition by a factor of ca. 3000). This effect was one order of magnitude higher when using lipofectamine as the transfection agent compared with TiO2 nanoparticles. The proposed (TiO2?PL)?Dz nanocomposites demonstrated high antiviral activity and are thus potent as nucleic-acid-based drugs.
    Copyright ? 2017. Published by Elsevier B.V.


    KEYWORDS:

    Cell delivery; DNAzymes; Gene silencing; Immobilization; Influenza A virus; TiO2-based nanocomposites

    PMID: 28412273 DOI: 10.1016/j.ijantimicag.2017.01.026
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