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Infect Immun. 2015 Apr 27. pii: IAI.00171-15. [Epub ahead of print]
Dynamic viral-bacterial interactions in a porcine precision-cut lung slice co-infection model: swine influenza virus paves the way for Streptococcus suis infection in a two-step process.
Meng F1, Wu NH1, Nerlich A2, Herrler G1, Valentin-Weigand P3, Seitz M2.
Author information
Abstract
Swine influenza virus (SIV) and Streptococcus suis are common pathogens of the respiratory tract in pigs, both associated with pneumonia. The interactions of both pathogens and their contribution to co-pathogenesis are only poorly understood. In the present study we established a porcine precision-cut lung slice co-infection model and analyzed the effects of a primary SIV infection on secondary infection by S. suis at different time points. We found that SIV promoted adherence, colonization, and invasion of S. suis in a two-step process. Firstly, in the initial stages, these effects were dependent on bacterial encapsulation, as shown by selective adherence of encapsulated, but not unencapsulated S. suis to SIV-infected cells. Secondly, at a later stage of infection SIV promoted S. suis adherence and invasion of deeper tissues by damaging ciliated epithelial cells. This effect was only seen with a highly virulent SIV subtype H3N2 strain, but not with a low virulent subtype H1N1 strain, and it was independent of the bacterial capsule, since an unencapsulated S. suis mutant behaved in a similar way as the encapsulated wild-type strain. Concluding, the porcine precision-cut lung slices (PCLS) co-infection model established here revealed novel insights into the dynamic interactions between SIV and S. suis during infection of the respiratory tract. It showed that at least two different mechanisms contribute to the beneficial effects of SIV for S. suis, including capsule-mediated bacterial attachment to SIV-infected cells and capsule-independent effects involving virus-mediated damage of ciliated epithelial cells.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25916988 [PubMed - as supplied by publisher]
Dynamic viral-bacterial interactions in a porcine precision-cut lung slice co-infection model: swine influenza virus paves the way for Streptococcus suis infection in a two-step process.
Meng F1, Wu NH1, Nerlich A2, Herrler G1, Valentin-Weigand P3, Seitz M2.
Author information
Abstract
Swine influenza virus (SIV) and Streptococcus suis are common pathogens of the respiratory tract in pigs, both associated with pneumonia. The interactions of both pathogens and their contribution to co-pathogenesis are only poorly understood. In the present study we established a porcine precision-cut lung slice co-infection model and analyzed the effects of a primary SIV infection on secondary infection by S. suis at different time points. We found that SIV promoted adherence, colonization, and invasion of S. suis in a two-step process. Firstly, in the initial stages, these effects were dependent on bacterial encapsulation, as shown by selective adherence of encapsulated, but not unencapsulated S. suis to SIV-infected cells. Secondly, at a later stage of infection SIV promoted S. suis adherence and invasion of deeper tissues by damaging ciliated epithelial cells. This effect was only seen with a highly virulent SIV subtype H3N2 strain, but not with a low virulent subtype H1N1 strain, and it was independent of the bacterial capsule, since an unencapsulated S. suis mutant behaved in a similar way as the encapsulated wild-type strain. Concluding, the porcine precision-cut lung slices (PCLS) co-infection model established here revealed novel insights into the dynamic interactions between SIV and S. suis during infection of the respiratory tract. It showed that at least two different mechanisms contribute to the beneficial effects of SIV for S. suis, including capsule-mediated bacterial attachment to SIV-infected cells and capsule-independent effects involving virus-mediated damage of ciliated epithelial cells.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25916988 [PubMed - as supplied by publisher]