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J Gen Virol. 2009 Jun 3. [Epub ahead of print]
Differential gene expression and host response profiles against avian influenza virus within the chicken lung due to anatomy and airflow.
Reemers SS, Van Haarlem DA, Groot Koerkamp MJ, Vervelde L. - Faculty Veterinary Medicine;
Sampling the complete organ instead of defined parts might affect the analysis at both cellular and transcriptional level. We defined host responses to H9N2 avian influenza virus (AIV) in trachea and different parts of the lung. Chickens were spray inoculated with either saline or H9N2 AIV. Trachea and lung were sampled at 1 and 3 days post inoculation (d.p.i.) for immunocytochemistry, real-time qRT-PCR and gene expression profiling.
The trachea was divided into upper and lower trachea and lung into 4 segments according to anatomy and airflow.
Two segments contained the primary and secondary bronchi cranial vs caudal (part L1 and L3) and two segments contained the tertiary bronchi cranial vs caudal (part L2 and L4).
Between upper and lower trachea in both control and infected birds minor differences in gene expression and host responses were found. In the lung of control birds differences in anatomy were reflected in gene expression, and in the lung of infected birds virus deposition enhanced the differences in gene expression.
Differential gene expression in trachea and lung suggested common responses to a wide range of agents and site specific responses. In trachea site specific response were related to heat shock and lysozyme activity. In lung L1, containing most virus, site specific responses related to genes involved in innate responses, interleukin activity and endocytosis.
Our study implicates that anatomy of the chicken lung has to be taken into account when investigating in vivo responses to respiratory virus infections.
PMID: 19494054 [PubMed - as supplied by publisher
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Differential gene expression and host response profiles against avian influenza virus within the chicken lung due to anatomy and airflow.
Reemers SS, Van Haarlem DA, Groot Koerkamp MJ, Vervelde L. - Faculty Veterinary Medicine;
Sampling the complete organ instead of defined parts might affect the analysis at both cellular and transcriptional level. We defined host responses to H9N2 avian influenza virus (AIV) in trachea and different parts of the lung. Chickens were spray inoculated with either saline or H9N2 AIV. Trachea and lung were sampled at 1 and 3 days post inoculation (d.p.i.) for immunocytochemistry, real-time qRT-PCR and gene expression profiling.
The trachea was divided into upper and lower trachea and lung into 4 segments according to anatomy and airflow.
Two segments contained the primary and secondary bronchi cranial vs caudal (part L1 and L3) and two segments contained the tertiary bronchi cranial vs caudal (part L2 and L4).
Between upper and lower trachea in both control and infected birds minor differences in gene expression and host responses were found. In the lung of control birds differences in anatomy were reflected in gene expression, and in the lung of infected birds virus deposition enhanced the differences in gene expression.
Differential gene expression in trachea and lung suggested common responses to a wide range of agents and site specific responses. In trachea site specific response were related to heat shock and lysozyme activity. In lung L1, containing most virus, site specific responses related to genes involved in innate responses, interleukin activity and endocytosis.
Our study implicates that anatomy of the chicken lung has to be taken into account when investigating in vivo responses to respiratory virus infections.
PMID: 19494054 [PubMed - as supplied by publisher
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