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Original Article
Citation: Emerging Microbes & Infections (2017) 6, e69; doi:10.1038/emi.2017.59
Published online 9 August 2017
Zika virus replication in the mosquito Culex quinquefasciatus in Brazil
OPEN
Duschinka RD Guedes1,*, Marcelo HS Paiva1,2,*, Mariana MA Donato1, Priscilla P Barbosa1, Larissa Krokovsky1, Sura W dos S Rocha1, Karina LA Saraiva1, M?nica M Crespo1, Tatiana MT Rezende1, Gabriel L Wallau1, Ros?ngela MR Barbosa1, Cl?udia MF Oliveira1, Maria AV Melo-Santos1, Lindomar Pena3, Marli T Cordeiro3, Rafael F de O Franca3, Andr? LS de Oliveira4, Christina A Peixoto1, Walter S Leal5 and Const?ncia FJ Ayres1
1Departamento de Entomologia, Instituto Aggeu Magalh?es, Funda??o Oswaldo Cruz (Fiocruz), Recife 50760-420, Brazil
2N?cleo de Ci?ncias da Vida, Centro Acad?mico do Agreste, Universidade Federal de Pernambuco, Caruaru 55002-970, Brazil
3Laborat?rio de Virologia e Terapia Experimental, Instituto Aggeu Magalh?es, Funda??o Oswaldo Cruz (Fiocruz), Recife 50670-420, Brazil
4N?cleo de Estat?stica e Geoprocessamento, Instituto Aggeu Magalh?es, Funda??o Oswaldo Cruz (Fiocruz), Recife 50670-420, Brazil
5Department of Molecular and Cellular Biology, University of California-Davis, Davis, CA 95616, USA
Correspondence: CFJ Ayres, E-mail: tans@cpqam.fiocruz.br
*These authors contributed equally to this work.
Received 14 November 2016; Revised 15 May 2017; Accepted 4 June 2017
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ABSTRACT
Zika virus (ZIKV) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. The virus is primarily transmitted by mosquito bite, although other routes of infection have been implicated in some cases. The Aedes aegypti mosquito is considered to be the main vector to humans worldwide; however, there is evidence that other mosquito species, including Culex quinquefasciatus, transmit the virus. To test the potential of Cx. quinquefasciatus to transmit ZIKV, we experimentally compared the vector competence of laboratory-reared Ae. aegypti and Cx. quinquefasciatus. Interestingly, we were able to detect the presence of ZIKV in the midgut, salivary glands and saliva of artificially fed Cx. quinquefasciatus. In addition, we collected ZIKV-infected Cx. quinquefasciatus from urban areas with high microcephaly incidence in Recife, Brazil. Corroborating our experimental data from artificially fed mosquitoes, ZIKV was isolated from field-caught Cx. quinquefasciatus, and its genome was partially sequenced. Collectively, these findings indicate that there may be a wider range of ZIKV vectors than anticipated.
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Original Article
Citation: Emerging Microbes & Infections (2017) 6, e69; doi:10.1038/emi.2017.59
Published online 9 August 2017
Zika virus replication in the mosquito Culex quinquefasciatus in Brazil
OPEN
Duschinka RD Guedes1,*, Marcelo HS Paiva1,2,*, Mariana MA Donato1, Priscilla P Barbosa1, Larissa Krokovsky1, Sura W dos S Rocha1, Karina LA Saraiva1, M?nica M Crespo1, Tatiana MT Rezende1, Gabriel L Wallau1, Ros?ngela MR Barbosa1, Cl?udia MF Oliveira1, Maria AV Melo-Santos1, Lindomar Pena3, Marli T Cordeiro3, Rafael F de O Franca3, Andr? LS de Oliveira4, Christina A Peixoto1, Walter S Leal5 and Const?ncia FJ Ayres1
1Departamento de Entomologia, Instituto Aggeu Magalh?es, Funda??o Oswaldo Cruz (Fiocruz), Recife 50760-420, Brazil
2N?cleo de Ci?ncias da Vida, Centro Acad?mico do Agreste, Universidade Federal de Pernambuco, Caruaru 55002-970, Brazil
3Laborat?rio de Virologia e Terapia Experimental, Instituto Aggeu Magalh?es, Funda??o Oswaldo Cruz (Fiocruz), Recife 50670-420, Brazil
4N?cleo de Estat?stica e Geoprocessamento, Instituto Aggeu Magalh?es, Funda??o Oswaldo Cruz (Fiocruz), Recife 50670-420, Brazil
5Department of Molecular and Cellular Biology, University of California-Davis, Davis, CA 95616, USA
Correspondence: CFJ Ayres, E-mail: tans@cpqam.fiocruz.br
*These authors contributed equally to this work.
Received 14 November 2016; Revised 15 May 2017; Accepted 4 June 2017
Top of page
ABSTRACT
Zika virus (ZIKV) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. The virus is primarily transmitted by mosquito bite, although other routes of infection have been implicated in some cases. The Aedes aegypti mosquito is considered to be the main vector to humans worldwide; however, there is evidence that other mosquito species, including Culex quinquefasciatus, transmit the virus. To test the potential of Cx. quinquefasciatus to transmit ZIKV, we experimentally compared the vector competence of laboratory-reared Ae. aegypti and Cx. quinquefasciatus. Interestingly, we were able to detect the presence of ZIKV in the midgut, salivary glands and saliva of artificially fed Cx. quinquefasciatus. In addition, we collected ZIKV-infected Cx. quinquefasciatus from urban areas with high microcephaly incidence in Recife, Brazil. Corroborating our experimental data from artificially fed mosquitoes, ZIKV was isolated from field-caught Cx. quinquefasciatus, and its genome was partially sequenced. Collectively, these findings indicate that there may be a wider range of ZIKV vectors than anticipated.
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