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mBio. Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia
[Source: mBio, full page: (LINK). Abstract, edited.]
Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia
Thomas Briese<SUP>a</SUP>, Nischay Mishra<SUP>a</SUP>, Komal Jain<SUP>a</SUP>, Iyad S. Zalmout<SUP>b</SUP>, Omar J. Jabado<SUP>c</SUP>, William B. Karesh<SUP>d</SUP>, Peter Daszak<SUP>d</SUP>, Osama B. Mohammed<SUP>b</SUP>, Abdulaziz N. Alagaili<SUP>b,e</SUP>, W. Ian Lipkin<SUP>a</SUP>
<SUP></SUP>
<SUP>a</SUP>Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA <SUP>b</SUP>KSU Mammals Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia <SUP>c</SUP>Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, New York, USA <SUP>d</SUP>EcoHealth Alliance, New York, New York, USA <SUP>e</SUP>Saudi Wildlife Authority, Riyadh, Saudi Arabia
Address correspondence to Thomas Briese, tb2047{at}cumc.columbia.edu, or Abdulaziz N. Alagaili, aziz99{at}gmail.com.
T.B. and N.M. contributed equally to this article.
Editor Michael Katze, University of Washington
ABSTRACT
Complete Middle East respiratory syndrome coronavirus (MERS-CoV) genome sequences were obtained from nasal swabs of dromedary camels sampled in the Kingdom of Saudi Arabia through direct analysis of nucleic acid extracts or following virus isolation in cell culture. Consensus dromedary MERS-CoV genome sequences were the same with either template source and identical to published human MERS-CoV sequences. However, in contrast to individual human cases, where only clonal genomic sequences are reported, detailed population analyses revealed the presence of more than one genomic variant in individual dromedaries. If humans are truly infected only with clonal virus populations, we must entertain a model for interspecies transmission of MERS-CoV wherein only specific genotypes are capable of passing bottleneck selection.
IMPORTANCE
In most cases of Middle East respiratory syndrome (MERS), the route for human infection with the causative agent, MERS coronavirus (MERS-CoV), is unknown. Antibodies to and viral nucleic acids of MERS-CoV have been found in dromedaries, suggesting the possibility that they may serve as a reservoir or vector for human infection. However, neither whole viral genomic sequence nor infectious virus has been isolated from dromedaries or other animals in Saudi Arabia. Here, we report recovery of MERS-CoV from nasal swabs of dromedaries, demonstrate that MERS-CoV whole-genome consensus sequences from dromedaries and humans are indistinguishable, and show that dromedaries can be simultaneously infected with more than one MERS-CoV. Together with data indicating widespread dromedary infection in the Kingdom of Saudi Arabia, these findings support the plausibility of a role for dromedaries in human infection.
Footnotes
Citation Briese T, Mishra N, Jain K, Zalmout IS, Jabado OJ, Karesh WB, Daszak P, Mohammed OB, Alagaili AN, Lipkin WI. 2014. Middle East respiratory syndrome coronavirus quasispecies that include homologues of human isolates revealed through whole-genome analysis and virus cultured from dromedary camels in Saudi Arabia. mBio 5(3):e01146-14. doi:10.1128/mBio.01146-14.
Received 9 April 2014 Accepted 16 April 2014 Published 29 April 2014
Copyright ? 2014 Briese et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Middle East Respiratory Syndrome Coronavirus Quasispecies That Include Homologues of Human Isolates Revealed through Whole-Genome Analysis and Virus Cultured from Dromedary Camels in Saudi Arabia
Thomas Briese<SUP>a</SUP>, Nischay Mishra<SUP>a</SUP>, Komal Jain<SUP>a</SUP>, Iyad S. Zalmout<SUP>b</SUP>, Omar J. Jabado<SUP>c</SUP>, William B. Karesh<SUP>d</SUP>, Peter Daszak<SUP>d</SUP>, Osama B. Mohammed<SUP>b</SUP>, Abdulaziz N. Alagaili<SUP>b,e</SUP>, W. Ian Lipkin<SUP>a</SUP>
<SUP></SUP>
<SUP>a</SUP>Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA <SUP>b</SUP>KSU Mammals Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia <SUP>c</SUP>Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, New York, USA <SUP>d</SUP>EcoHealth Alliance, New York, New York, USA <SUP>e</SUP>Saudi Wildlife Authority, Riyadh, Saudi Arabia
Address correspondence to Thomas Briese, tb2047{at}cumc.columbia.edu, or Abdulaziz N. Alagaili, aziz99{at}gmail.com.
T.B. and N.M. contributed equally to this article.
Editor Michael Katze, University of Washington
ABSTRACT
Complete Middle East respiratory syndrome coronavirus (MERS-CoV) genome sequences were obtained from nasal swabs of dromedary camels sampled in the Kingdom of Saudi Arabia through direct analysis of nucleic acid extracts or following virus isolation in cell culture. Consensus dromedary MERS-CoV genome sequences were the same with either template source and identical to published human MERS-CoV sequences. However, in contrast to individual human cases, where only clonal genomic sequences are reported, detailed population analyses revealed the presence of more than one genomic variant in individual dromedaries. If humans are truly infected only with clonal virus populations, we must entertain a model for interspecies transmission of MERS-CoV wherein only specific genotypes are capable of passing bottleneck selection.
IMPORTANCE
In most cases of Middle East respiratory syndrome (MERS), the route for human infection with the causative agent, MERS coronavirus (MERS-CoV), is unknown. Antibodies to and viral nucleic acids of MERS-CoV have been found in dromedaries, suggesting the possibility that they may serve as a reservoir or vector for human infection. However, neither whole viral genomic sequence nor infectious virus has been isolated from dromedaries or other animals in Saudi Arabia. Here, we report recovery of MERS-CoV from nasal swabs of dromedaries, demonstrate that MERS-CoV whole-genome consensus sequences from dromedaries and humans are indistinguishable, and show that dromedaries can be simultaneously infected with more than one MERS-CoV. Together with data indicating widespread dromedary infection in the Kingdom of Saudi Arabia, these findings support the plausibility of a role for dromedaries in human infection.
Footnotes
Citation Briese T, Mishra N, Jain K, Zalmout IS, Jabado OJ, Karesh WB, Daszak P, Mohammed OB, Alagaili AN, Lipkin WI. 2014. Middle East respiratory syndrome coronavirus quasispecies that include homologues of human isolates revealed through whole-genome analysis and virus cultured from dromedary camels in Saudi Arabia. mBio 5(3):e01146-14. doi:10.1128/mBio.01146-14.
Received 9 April 2014 Accepted 16 April 2014 Published 29 April 2014
Copyright ? 2014 Briese et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
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