EID Journal Home > Volume 16, Number 12–December 2010

Research

Environmental Sampling for Avian Influenza Virus A (H5N1) in Live-Bird Markets, Indonesia


Abstract
To identify environmental sites commonly contaminated by avian influenza virus A (H5N1) in live-bird markets in Indonesia, we investigated 83 markets in 3 provinces in Indonesia.

At each market, samples were collected from up to 27 poultry-related sites to assess the extent of contamination. Samples were tested by using real-time reverse transcription–PCR and virus isolation. A questionnaire was used to ascertain types of birds in the market, general infrastructure, and work practices.

Thirty-nine (47%) markets showed contamination with avian influenza virus in >1 of the sites sampled.

Risk factors were slaughtering birds in the market and being located in West Java province. Protective factors included daily removal of waste and zoning that segregated poultry-related work flow areas.

These results can aid in the design of evidence-based programs concerning environmental sanitation, food safety, and surveillance to reduce the risk for avian influenza virus A (H5N1) transmission in live-bird markets.

- snip -

Our findings provide further evidence that environmental contamination with AIVs is not uncommon (8,14). Poultry water, drains, tabletops, cages, tablecloths, utensils, bins, and floors were all contaminated.

Full Article