EID Journal Home > Volume 16, Number 12?December 2010
Research
Environmental Sampling for Avian Influenza Virus A (H5N1) in Live-Bird Markets, Indonesia
Abstract
To identify environmental sites commonly contaminated by avian influenza virus A (H5N1) in live-bird markets in Indonesia, we investigated 83 markets in 3 provinces in Indonesia.
At each market, samples were collected from up to 27 poultry-related sites to assess the extent of contamination. Samples were tested by using real-time reverse transcription?PCR and virus isolation. A questionnaire was used to ascertain types of birds in the market, general infrastructure, and work practices.
Thirty-nine (47%) markets showed contamination with avian influenza virus in >1 of the sites sampled.
Risk factors were slaughtering birds in the market and being located in West Java province. Protective factors included daily removal of waste and zoning that segregated poultry-related work flow areas.
These results can aid in the design of evidence-based programs concerning environmental sanitation, food safety, and surveillance to reduce the risk for avian influenza virus A (H5N1) transmission in live-bird markets.
- snip -
Our findings provide further evidence that environmental contamination with AIVs is not uncommon (8,14). Poultry water, drains, tabletops, cages, tablecloths, utensils, bins, and floors were all contaminated.
Full Article
Research
Environmental Sampling for Avian Influenza Virus A (H5N1) in Live-Bird Markets, Indonesia
Abstract
To identify environmental sites commonly contaminated by avian influenza virus A (H5N1) in live-bird markets in Indonesia, we investigated 83 markets in 3 provinces in Indonesia.
At each market, samples were collected from up to 27 poultry-related sites to assess the extent of contamination. Samples were tested by using real-time reverse transcription?PCR and virus isolation. A questionnaire was used to ascertain types of birds in the market, general infrastructure, and work practices.
Thirty-nine (47%) markets showed contamination with avian influenza virus in >1 of the sites sampled.
Risk factors were slaughtering birds in the market and being located in West Java province. Protective factors included daily removal of waste and zoning that segregated poultry-related work flow areas.
These results can aid in the design of evidence-based programs concerning environmental sanitation, food safety, and surveillance to reduce the risk for avian influenza virus A (H5N1) transmission in live-bird markets.
- snip -
Our findings provide further evidence that environmental contamination with AIVs is not uncommon (8,14). Poultry water, drains, tabletops, cages, tablecloths, utensils, bins, and floors were all contaminated.
Full Article