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Buletin Veteriner Udayana: THE ROLE OF CODING AND NON-CODING REGION OF POLYMERASE GENE COMPLEX IN ADAPTATION OF AVIAN INFLUENZA VIRUS

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  • Buletin Veteriner Udayana: THE ROLE OF CODING AND NON-CODING REGION OF POLYMERASE GENE COMPLEX IN ADAPTATION OF AVIAN INFLUENZA VIRUS

    Buletin Veteriner Udayana Vol. 4 No.1. Pebruari 2012

    THE ROLE OF CODING AND NON-CODING REGION OF POLYMERASE GENE COMPLEX IN ADAPTATION OF AVIAN INFLUENZA VIRUS


    Peran Coding dan Non-Coding Region dari Gen Polimerase Kompleks Dalam Adaptasi Virus Avian Influenza


    Gusti Ayu Yuniati Kencana

    Abstract

    Avian influenza (AI) is an avian disease that can cause deadly human infection. The disease is caused by Orthomyxoviridae virus, genus influenza virus type A, subtype H5N1. Pathogenicity of AI virus is polygenic, which means AI virus is determined by all the genes. Here the role of coding region (CR) and non-coding region (NCR) of polymerase gene complex is critically reviewed. The coding region of the polymerase genes can be explained as follows. The amino acid position 627 PB2 gene is a factor adaptation of AI viruses in mammals. Polymerase basic-1 (PB1) protein acts as a central activity of the enzyme catalyzing the viral polymerase. Polymerase Basic-1 binds to the terminal end of the vRNA and the cRNA and shows endo-nuclease activity. The activity has been identified on the E508, E519, and D522. Polymerase Acid (PA) proteins play a role in supporting the biological activity of the polymerase gene complex, but its mechanism of action in transcription, replication has not been disclosed as clear. Another role of the PA protein is forming a complex of RNA polymerase and express an proteolytic role of cell proteins that suppress cell division. Polymerase acid gene has alsoserine protease activity has been identified at position S624. The non-coding region might also play role in the pathogenecity of influenza virus. The results of sequence analysis of non-coding region (NCR) at 5-end of polymerase gene complex of AI virus subtype H5N1 from poultry and pigs in Indonesia showed that the NCR genes PB2, PB1, and PA are homogeneous, whereas there are variants of the PB1 gene isolates from ducks A / Duck / Badung, 2006. Occurrence of deletions, insertions, and mutations in the NCR and CR polymerase complex genes may likely lead to genetic changes in viruses which potentially also change the nature of biology of AI virus. The study on the 3-end of the genes needs to be carried out.

    Keywords

    coding region, non-coding region, polymerase gene complex, Avian Influenza

    http://ojs.unud.ac.id/index.php/bule...le/view/2786/0

    Full text in Bahasa Indonesia: PDF
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