225G Worldwide Tracking & Evaluation

[mixin]

Re: 225G Preliminary Worldwide Tracking & Evaluation

Interesting that this PLoS study used egg passage.

I can't find a way to cut/paste this picture: Figure S6.

If I understand this correctly they innoculated with mixed signals D225X and Q226X (grT and crA).

In the sample taken from the directly innoculated pigs, D225X remained, but 226X reverted to Q226.

When transmitted to other pigs, both reverted to D225 (gat) and Q226 (caa)

So during transmission, it mutated from mixed signals to non-mutated signals?

But then in the lungs, on 1 and 2 days post-infection, it was still D225X and Q226 but on days 4 and 7, it changed to D225G and remained Q226.

Pig 3261 had to be put to death unexpectedly on day 17 due to welfare issues.

[mixin]

Re: 225G Preliminary Worldwide Tracking & Evaluation

Here's the picture. HT to gs to use printscreen..

[mixin]

Re: 225G Preliminary Worldwide Tracking & Evaluation

Brazil released a series of sequences from late last summer at GenBank.
These are all from deceased patients, some have D225 mutations (715, 716).

A/Rio de Janeiro/14601/2009 24-Aug-2009 gender M; age 23Y Nasopharyngeal aspirate
A4G(4),C537T(4),A716G(4),G930T(4),T1275C(4),C1408T (4)

A/Rio de Janeiro/4707/2009 23-Jul-2009 gender F; age 31Y Nasopharyngeal aspirate
A716G(4),G930T(4),C1408T(4),G1556A(4)

A/Rio de Janeiro/7131/2009 01-Aug-2009 gender F; age 16Y Nasopharyngeal aspirate
A4G(4),A232T(4),A716G(4),G930T(4),G1080A(4),C1408T (4)

A/Rio de Janeiro/7455/2009 04-Aug-2009 gender M; age 37Y Nasopharyngeal aspirate
A4G(4),A716G(4),G930T(4),A1272G(4),A1394C(4),C1408 T(4)

A/Bahia/15525/2009 05-Sep-2009 gender M; age 42Y Nasopharyngeal aspirate
T141C(4),G340A(4),T658A(4),G687A(4),G715R(4),A716R (4),C1408T(4),C1476Y(4),C1539Y(4),C1574Y(4)

A/Bahia/12606/2009 18-Aug-2009 gender F; age 38Y Nasopharyngeal secretion
C309A(4),G318A(4),T658A(4),A1281G(4),G1299A(4),C14 08T(4),

A/Rio Grande do Sul/9116/2009 02-Aug-2009 gender M; age 39Y Nasopharyngeal aspirate
G930T(4),A1260R(4),C1408T(4),

A/Minas Gerais/14602/2009 22-Aug-2009 gender F; age 36Y Nasopharyngeal secretion
T658A(4),C1408T(4),

A/Santa Catarina/6235/2009 26-Jul-2009 gender M; age 42Y Nasopharyngeal aspirate
G930T(4),C1408T(4),T1712C(4),C1713A(4),A1714G(4),G 1715A(4),A1717G(4),G1718C(4),C1719A(4),

[gsgs]

Re: 225G Preliminary Worldwide Tracking & Evaluation

the first 4 don't have T658A thus are pre-Cancun.
Still 2 of them have A716G - yet another background,
yet another evidence that it developes in the host and
(usually) doesn't spread.

I'll update my South-America table in the genbank thread

[gsgs]

Re: 225G Preliminary Worldwide Tracking & Evaluation

http://www.ncbi.nlm.nih.gov/pubmed/20367331

Quasispecies of the D225G Substitution in the Hemagglutinin of Pandemic
Influenza A(H1N1) 2009 Virus from Patients with Severe Disease in Hong Kong, China.
D225G substitution in 7 of 57 patients with severe disease and for 0 of 60 with mild disease

what does Nancy Cox say ?

[mixin]

Re: 225G Preliminary Worldwide Tracking & Evaluation

Latest update for GenBank sequences with mutations at 716 = D225G and mixed signals. gs.

G >A/Mexico/InDRE4114,2009//
G >A/Mexico/InDRE4114,2009//
T >A/Monterrey/3,2009//
G >A/Abakan/02,2009//
G >A/Salekhard/01,2009//
G >A/Tomsk/07,2009//
G >A/Tomsk/08,2009//
G >A/Vladivostok/01,2009//
R >A/Sw/4/Mexico,2009/04/
R >A/Nebraska/02,2009/04/
G >A/NY/04,2009/04/
G >A/Mexico/3955,2009/04/02
R >A/CA/07,2009/04/09
R >A/Texas/04,2009/04/14
G >A/Texas/05,2009/04/15
R >A/CA/13,2009/04/21
R >A/Texas/10,2009/04/23
G >A/Texas/11,2009/04/23
R >A/NY/31,2009/04/24
R >A/NY/11,2009/04/25
G >A/Georgia/01,2009/04/27
T >A/Sw/Alberta/OTH-33-3,2009/05/03
G >A/Hiroshima/201,2009/06/17
M >A/CA/VRDL31,2009/06/17
G >A/CA/VRDL7,2009/06/17
G >A/CA/VRDL27,2009/06/27
G >A/Argentina/HNRG16,2009/06/29
R >A/Texas/42291877,2009/06/29
G >A/Sao Paulo/53206,2009/07/19
G >A/Rio de Janeiro/4707,2009/07/23
R >A/Finland/614,2009/07/24
R >A/Utah/42,2009/07/24
G >A/Utah/42,2009/07/24
G >A/Rio de Janeiro/5826,2009/07/28
G >A/Catalonia/NS1706,2009/07/29
N >A/Singapore/GP2312,2009/07/31
G >A/Pune/NIV9355,2009/08/
G >A/Rio de Janeiro/7131,2009/08/01
G >A/Sao Paulo/53225,2009/08/01
G >A/Catalonia/NS2001,2009/08/03
G >A/Catalonia/NS2008,2009/08/03
G >A/Rio de Janeiro/7455,2009/08/04
N >A/Singapore/GP2641,2009/08/05
N >A/Singapore/GP2651,2009/08/05
R >A/Norway/2924,2009/08/10
G >A/Rio de Janeiro/14601,2009/08/24
G >A/Roma/ISS1897,2009/08/27
G >A/Roma/ISS1941,2009/08/27
R >A/Nagano/RC1,2009/08/27
G >A/Pune/NIV10278,2009/09/
G >A/Norway/3206-3,2009/09/01
R >A/Bahia/15525,2009/09/05
G >A/Zhejiang-Yiwu/11,2009/09/06
G >A/Zhejiang/DTID-ZJU02,2009/09/07
G >A/Zhejiang/DTID-ZJU03,2009/09/07
R >A/Mexico/InDRE43149,2009/09/28
R >A/Mexico/InDRE50625,2009/10/31
G >A/Egypt/N14644,2009/11/01
R >A/Mexico/InDRE50617,2009/11/01
G >A/Bryansk/IIV2971,2009/11/11
G >A/Tver/IIV2969,2009/11/14
G >A/Thessaloniki/2502,2009/11/17
G >A/Kastoria/2636,2009/11/18
G >A/Thessaloniki/2812,2009/11/22
G >A/Perm/01,2009/11/25
G >A/Tver/IIV-183,2009/11/25
G >A/Taraz/01,2009/12/
G >A/IIV-Anadyr/177,2009/12/04
G >A/Yaroslavl/IIV-196,2009/12/04
G >A/Catalonia/NS092145684,2009/12/10
R >A/NY/7216,2009/12/21
G >A/Karasuk/01,2010/01/04
G >A/Kavala/219,2010/01/12
G >A/Thessaloniki/206,2010/01/12
G >A/Thessaloniki/225,2010/01/13
G >A/Orenburg/IIV-13,2010/03/02
716 mxha9 76 -:126 A:2971 G:52 M:1 N:3 R:18 T:2

[Ronan Kelly]

Re: 225G Preliminary Worldwide Tracking & Evaluation

hattip HenryN @RLFT
Volume 16, Number 5–May 2010
Dispatch
Transmission of Hemagglutinin D222G Mutant Strain of Pandemic (H1N1) 2009 Virus
Simona Puzelli, Marzia Facchini, Domenico Spagnolo, Maria A. De Marco, Laura Calzoletti, Alessandro Zanetti, Roberto Fumagalli, Maria L. Tanzi, Antonio Cassone, Giovanni Rezza, Isabella Donatelli, and the Surveillance Group for Pandemic A (H1N1) 2009 Influenza Virus in Italy1
Author affiliations: National Institute of Health, Rome, Italy (S. Puzelli, M. Facchini, D. Spagnolo, M.A. De Marco, L. Calzoletti, A. Cassone, G. Rezza, I. Donatelli); Università degli Studi di Milano, Milan, Italy (A. Zanetti); Università degli Studi di Milano-Bicocca, Milan (R. Fumagalli); and Università degli Studi di Parma, Parma, Italy (M.L. Tanzi)

Suggested citation for this article

Abstract
A pandemic (H1N1) 2009 virus strain carrying the D222G mutation was identified in a severely ill man and was transmitted to a household contact. Only mild illness developed in the contact, despite his obesity and diabetes. The isolated virus reacted fully with an antiserum against the pandemic vaccine strain.
On November 20, 2009, the Norwegian Institute of Public Health reported to the World Health Organization a mutation in the hemagglutinin (HA) of pandemic (H1N1) 2009 virus, consisting in a change of aspartic acid (D) with glycine (G) at aa 222. The mutation had been detected in 3 patients (the first 2 fatal cases in the country and in 1 patient with severe pneumonia) among ≈70 other patients with pandemic (H1N1) 2009, suggesting that it was not widespread in Norway (1). The same mutation has been also detected in Brazil, China, Japan, Mexico, Ukraine, the United States, France, and Spain (1,2).

The D-to-G mutation among the 1918 influenza virus variants (3) correlated with a shift from α2-6–linked sialic acid preference to a dual α2-3/α2-6 specificity. However, whether such a mutation may alter receptor binding specificity in the pandemic (H1N1) 2009 virus is unknown.

Although several pandemic (H1N1) 2009 viral strains sharing this mutation were detected in fatal cases, the same mutation also was detected in some mild cases; conversely, viruses from numerous fatal cases have not shown the same mutation. Thus, the clinical and public health significance of this finding remains unclear. The mutation appears to occur sporadically and spontaneously. No links between the small number of patients infected with the mutated virus have been found, and the mutation did not appear to spread (4). On the basis of results from a retrospective HA1 sequence analysis performed on pandemic (H1N1) 2009 viral isolates in Italy, we report on a transmission event of this virus carrying the D222G mutation.

The Study
We reexamined the HA sequences of 130 influenza A (H1N1) virus strains identified from patients affected by pandemic (H1N1) 2009. The neuraminidase sequences of some of these viruses also have been analyzed.

Figure Link to picture:

http://www.cdc.gov/eid/content/16/5/863-F.htm

Figure. Phylogenetic relationships of hemagglutinin (HA) 1 sequences of pandemic (H1N1) 2009 viruses in Italy obtained from the National Influenza Centre (NIC)–Istituto Superiore di Sanità (ISS) and the National Center for Biotechnology Information...


All 130 strains had been obtained from clinical samples (nasal, pharyngeal, or nasopharyngeal swabs and/or tracheal aspirates) collected during May–November 2009 in the context of virologic surveillance conducted by the National Influenza Centre, in collaboration with the regional laboratory network. These samples were obtained to study the evolution of the pandemic strain. Forty-one HA gene sequences examined in the present study were retrieved from the National Center for Biotechnology Information, 3 from the Global Initiative on Sharing Avian Influenza Data database, and 86 HA sequences (47 directly from the clinical samples and 39 from cell culture supernatant) were obtained at the National Influenza Centre (NIC) with the following procedure. Viral RNAs were extracted by using the QIAamp Viral RNA Mini Kit (QIAGEN, Santa Clara, CA, USA) and amplified by reverse transcription–PCR (RT-PCR) (5). HA amplicons were sequenced by using the BigDye Terminator Cycle-Sequencing Ready Reaction (Applied Biosystems, Foster City, CA, USA) and ABI Prism 310 DNA sequencer (Applied Biosystems). All the NIC sequences were deposited in the GenBank database under the accession numbers reported in the Figure. Sequences were assembled and aligned using Lasergene package, version 4.0 (DNASTAR, Madison, WI, USA). BioEdit software version 4.0 (www.mbio.ncsu.edu/BioEdit/bioedit.html) of the MEGA software package (www.megasoftware.net) was used to estimate phylogenies from the nucleotide sequences and to construct phylogenetic trees by using the neighbor-joining algorithm and maximum-likelihood method.

Among 130 patients, 23 of whom had severe disease (i.e., requiring hospitalization), only 1 was infected with a virus showing the D222G change. The patient, a man 25 years of age from northern Italy, had a febrile illness on August 17. One week later, he was admitted to an intensive care unit with severe pneumonia and acute respiratory distress syndrome, which resolved after treatment with extracorporeal membrane oxygenation. Influenza A (H1N1) viral genome showing the D222G mutation was identified through direct sequencing of nasopharyngeal swab and tracheal aspirate collected on August 27. No sequence could be retrieved from a nasal wash, which was obtained the same day and was positive in real-time RT–PCR for pandemic (H1N1) 2009 virus. No viral growth was detected in MDCK cells seeded with both clinical samples.

To identify possible transmission chains of the mutated virus, we analyzed the genome of the viral strain detected in the throat swab of the father of the index case-patient, 55 years of age, who was obese and had diabetes. He became moderately ill on August 25 but did not require hospitalization or antiviral treatment. The virus isolated in MDCK cells from the sample obtained on August 27 had the same HA mutation, D222G. Viral strains from index and contact cases were susceptible to oseltamivir, as determined by lack of the specific oseltamivir-resistance marker (His274Tyr, N2 numbering) in neuraminidase sequences, which were identical in both viral strains. Additional samples analyzed from close contacts (i.e., 4 healthcare workers, 4 family members, and 2 friends) of the 2 patients all were negative for pandemic (H1N1) 2009 virus.

Comparison of the 2 HA1 sequences from the index case-patient and his father showed an additional substitution in the latter (G155E), which is located close to the receptor-binding pocket. Furthermore, sequence analyses showed that all virus strains containing a change in HA1 position 222 (D222G or D222E) also showed a second substitution in position 203 (D203T), when compared with the A/California/7/2009 vaccine strain (Figure). However, the effect of this second mutation on the HA receptor-binding properties is still unclear. The HA1 genes from the index and contact case-patients shared 2 additional nucleotide changes, 1 synonymous substitution (T504C, N1 numbering) not found in any other sequence analyzed in the present study and another nucleotide change resulting in an amino acid substitution (P297S, N1 numbering), detected only in a small number of sequences from Italy. Hemagglutination-inhibition test of the isolated virus did not show substantial reduction in its reactivity with an antiserum against pandemic (H1N1) 2009 vaccine, as compared with the reactivity of an influenza virus A/California/7/2009 strain not carrying the G155E mutation (Table). Link: http://www.cdc.gov/EID/content/16/5/863-T.htm


Conclusions
Identification of a pandemic (H1N1) 2009 virus strain carrying the D222G mutation and its association with the first fatal cases of influenza in Norway raised some concern about emergence of a viral strain with increased pathogenicity. No data have been reported on transmission capacity of this and other D222G variants, occasionally identified worldwide. Our findings suggest that the D222G mutated virus is to some extent transmissible. However, a number of close contacts were identified who did not acquire the infection.
Whether the mutated virus may have a lower fitness for receptors in the high respiratory tract, which may affect transmission, remains undefined. Furthermore, the mutation, which was first found in a severely affected man, was transmitted to a family member (father), who had only a mild illness, despite risk for severe infection. The HA1 sequence from this latter case presented an additional amino acid substitution (G155E), which was not found in any other sequence analyzed in our study and rarely detected among all sequences available from GenBank. Recent data suggest that amino acid substitution at this position may be critical for the switch from dual α2-3/α2-6 binding specificity to α2-6 linkage (6), which is preferentially recognized by human influenza viruses and expressed mainly in the upper respiratory tract. This change might be partially responsible for the milder influenza illness developed by the father of the index case-patient. In addition, the G155E mutation in laboratory-generated variants of pandemic (H1N1) 2009 virus has been associated with loss of antigenicity (7). In our study, the natural isolates of the virus carrying both the G155E and the D222G mutations had comparable antigenicity with the A/California/7/09-vaccine strain. Finally, our data do not support the association of the D222G mutation with severe disease.
Acknowledgments
We thank Tiziana Grisetti for editing the manuscript and F. Fazio for encouragement and support.

This study was supported by an Italian Ministry of Health research grant.

Dr Puzelli is a research biologist in the Department of Infectious, Parasitic and Immune-mediated Diseases at the National Institute of Health, Rome, Italy. Her research interests include molecular mechanisms of genetic variability of influenza viruses and antiviral susceptibility.

References
1.World Health Organization. Public health significance of virus mutation detected in Norway. Pandemic (H1N1) 2009. Briefing note 17 [cited 2009 Nov 20]. http://www.who.int/csr/disease/swine...ng_20091120/en
2.European Centre for Disease Prevention and Control Daily Update. Pandemic (H1N1) 2009 [cited 2009 Nov 30]. http://ecdc.europa.eu/en/healthtopic...rt_0900hrs.pdf
3.Stevens J, Blixt O, Glaser L, Taubenberger JK, Palese P, Paulson JC, et al. Glycan microarray analysis of the hemagglutinins from modern and pandemic influenza viruses reveals different receptor specificities. J Mol Biol. 2006;355:1143–55. PubMed DOI
4.World Health Organization. Preliminary review of D222G amino acid substitution in the haemagglutinin of pandemic influenza A (H1N1) 2009 viruses [cited 2010 Mar 22]. http://www.who.int/csr/resources/pub.../en/index.html
5.Surveillance Group for New Influenza A. Virological surveillance of human cases of influenza A(H1N1)v virus in Italy: preliminary results. Euro Surveill. 2009;14:19247.
6.Takahashi T, Hashimoto A, Maruyama M, Ishida H, Kiso M, Kawaoka Y, et al. Identification of amino acid residues of influenza A virus H3 HA contributing to the recognition of molecular species of sialic acid. FEBS Lett. 2009;583:3171–4. PubMed DOI
7.Chen Z, Wang W, Zhou H, Suguitan AL Jr, Shambaugh C, Kim L, et al. Generation of live attenuated novel influenza virus A/California/7/09 (H1N1) vaccines with high yield in embryonated chicken eggs. J Virol. 2010;84:44–51.
Figure
Figure. Phylogenetic relationships of hemagglutinin (HA) 1 sequences of pandemic (H1N1) 2009 viruses in Italy obtained from the National Influenza Centre (NIC)–Istituto Superiore di Sanità (ISS) and the National Center for Biotechnology Information...

Table Link: http://www.cdc.gov/EID/content/16/5/863-T.htm
Table. Hemagglutination-inhibition test results of pandemic (H1N1) 2009 viruses, Italy

Suggested Citation for this Article
Puzelli S, Facchini M, Spagnolo D, De Marco MA, Calzoletti L, Zanetti A, et al. Transmission of hemagglutinin D222G mutant strain of pandemic (H1N1) 2009 virus. Emerg Infect Dis [serial on the Internet]. 2010 May [date cited]. http://www.cdc.gov/EID/content/16/5/863.htm

DOI: 10.3201/eid1605.091858



1Members of the Surveillance Group for Pandemic A(H1N1) 2009 Influenza Virus in Italy: Istituto Superiore di Sanitá (ISS) (National Institute of Health): Livia Di Trani, Annapina Palmieri, and Concetta Fabiani; Regional Laboratories (director name in parentheses): Istituto di ricovero e cura a carattere scientifico, Rome (Maria R. Capobianchi); Catholic University, Rome (Giovanni Fadda); Padua (Giorgio Palù Florence (Alberta Azzi); Palermo (Fabio Tramuto); Triest (Pierlanfranco D'Agaro); Cosenza (Cristina Giraldi); Naples (Ciro Esposito); Ancona (Patrizia Bagnarelli); Turin (Valeria Ghisetti); and Genoa (Filippo Ansaldi).

http://www.cdc.gov/eid/content/16/5/863.htm

[mixin]

Re: 225G Preliminary Worldwide Tracking & Evaluation

Updated mutations at 715 = D225N

C:\gs>travel1 mxha9 715
T >A/Monterrey/3,2009//
R >A/Sw/4/Mexico,2009/04/
R >A/South Carolina/09,2009/04/26
R >A/Arizona/04,2009/04/27
A >A/Argentina/HNRG8,2009/06/11
A >A/NY/4099,2009/06/13
A >A/NY/4576,2009/07/08
A >A/Texas/43132503,2009/07/13
R >A/Utah/42,2009/07/24
A >A/Sao Paulo/53838,2009/08/01
A >A/Sao Paulo/53845,2009/08/02
A >A/Malaysia/8860,2009/08/08
R >A/Mexico/InDRE36529,2009/08/08
R >A/Nagano/RC1,2009/08/27
A >A/Nagano/RC1,2009/08/27
R >A/Bahia/15525,2009/09/05
R >A/Mexico/InDRE41741,2009/09/14
R >A/Mexico/InDRE50625,2009/10/31
A >A/Egypt/N14648,2009/11/
R >A/Mexico/InDRE50617,2009/11/01
A >A/Russia/100,2009/11/01
A >A/Orenburg/IIV2974,2009/11/16
A >A/Ankara/26,2009/11/24
A >A/Yaroslavl/IIV-198,2009/12/05
24 -:126 A:13 G:3023 R:10 T:1

[gsgs]

Re: 225G Preliminary Worldwide Tracking & Evaluation

http://www.ncbi.nlm.nih.gov/pubmed/20718801

Surveillance of autopsy cases for D222G in Alberta, Canada
some specimens from autopsy patients were positive for D222N, none were positive for D222G

[tetano]

225G Preliminary Worldwide Tracking & Evaluation

Clin Microbiol Infect. 2010 Oct 14. doi: 10.1111/j.1469-0691.2010.03403.x. [Epub ahead of print]
Severe outcome of influenza A/H1N1/09v infection associated with 222G/N polymorphisms in the haemagglutinin: a multicenter study.

Baldanti F, Campanini G, Piralla A, Rovida F, Braschi A, Mojoli F, Iotti G, Belliato M, Conaldi PG, Arcadipane A, Pariani E, Zanetti A, Minoli L, Emmi V.

  Molecular Virology Unit  Intensive Care Unit I  Intensive Care Unit II, Fondazione IRCCS Policlinico San Matteo, Pavia  Institute of Microbiology and Virology  Intensive Care Unit, ISSMET, Palermo  Dipartimento di Sanit? Pubblica-Microbiologia-Virologia, Universit? degli Studi di Milano, Milan  Institute of Infectious Diseases, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy.
Abstract

In a multicenter study influenza A/H1N1/09v 222G/N variants were more frequently detected in patients admitted to intensive care unit (ICU) for invasive mechanical ventilation or extracorporeal membrane oxygenation (ECMO) (10/23; 43.5%) than in patients hospitalized in other units (2/27; 7.4%) and community patients (0/81; 0.0%) (p<0.01). A significantly higher virus load (p=0.02) in the lower vs upper respiratory tract was observed. Predominance of 222G/N variants in the lower respiratory tract (40% of total virus population) vs upper respiratory tract (10%) was shown by clonal analysis of HA sequences in paired nasal swab and bronchoalveolar lavage samples. The time from illness onset to sampling was significantly longer in patients with severe infection vs community patients (p<0.001). Conclusions: i) 222G/N variants showed increased virulence; ii) mutant variants were likely selected in individual patients, iii) the longer duration of illness might have favored the emergence of adaptive mutations through multiple replication cycles.
Copyright ? 2010 European Society of Clinical Microbiology and Infectious.

PMID: 20946414 [PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/pubmed/20946414

[NS1]

HA 225G with 158E Series in Jiangsu China

HA 225G with 158E Series in Jiangsu China

http://pf11.blogspot.com/2010/12/ha-225g-with-158e-series-in-jiangsu.html

Last Updated 2010-12-15

The Jiangsu Provincial Center for Disease Prevention and Control released a small set of sequences at GenBank on 2010-12-06 from the late 2009 pH1N1 reservoir. These sequences are related to several of the most recent and most concerning Chinese sequences on file that also came from Jiangsu. In an unusual presentation, the early sequences appear to be more elaborate genetically than the later group. Are we seeing the PF11 reservoir select the most useful revisions to continue attacking humans while dropping extraneous or sub-optimal genetic material?

Recall that the Ukraine produced an early fatal Vaccine Escape strain, UkrLvivN6_26M_2009_10_27_xL_VxX_f, that carried only one amino acid change, 225G, and one silent change, syn413K. A variant passage of that same UkrLvivN6 patient created a sequence, UkrLvivN6_2009_10_27_C3C1_xL_f, with an additional amino acid change producing the following polymorphisms.


UkrLvivN6_2009_10_27_C3C1_xL_f

• 158E
• 225G
• syn413K (AAg) - synonymous cross-linkage (xL) marker

The 158E and 225G amino acid combination came to be of interest to GeneWurx due to the variant sub-species produced from this Ukrainian sample. As additional sequences emerged in a similar geography including RussiaBelgorod2_2010_03_15 as predicted with 230I, emergent behaviour around these amino acid positions was noted for closer surveillance and the diverse nucleotide coding for 225G (Gga) from an overlay of 225G (GgT) onto a 225E (GAa) substrate was packaged onto the corresponding watch list.

The Chinese sequences discussed today have a limited set of metadata, and are perhaps more highly correlated than they appear. A slight possibility for duplicate submission exists. Six geographically-sited sequences with this type of combination is a set worthy of investigation. Although some potential exists for errors in the names and dates of these Chinese sequences, the data at hand suggests that a sub-clade may be emerging in the Jiangsu region with five polymorphisms traveling together in a group that boasts rarity and Vaccine Escape documentation.

Jiangsu province is on the east coast of China and boasts the highest population density of any province in the country. Water covers 18% of the province with another 68% as low-lying plains. Jiangsu is also known as the ?land of water? due to a thousand kilometer coast line with the Yellow Sea, the Yangtze River passing through the south, an extensive irrigation system and the canals found throughout several of the cities. Nanjing, the capital, is situated within the Yangtze River Delta. [Jiangsu, Nanjing]

With this deposit, polymorphisms at amino acid position 454 may now be correlated with RBD changes. An affinity exists in the aa223 to aa226 range and with HA 188T. These Chinese sequences carry the 158E revision that is useful for infecting the human upper respiratory tract and the 225G that is equally as useful in deep lung infection.

The recent 230I bearing sequence from Russia also demonstrates that 158E and 225G combination. A more current sequence from Australia, OzBrisbane209_51F_2010_08_09, shows a tendency toward this framing via domaining with 156E and matching the 225G while also carrying the H7N7 viral conjunctivitis-correlated polymorphism of HA 188T. Perhaps the SNP coding for our Southern Hemisphere 156E will be discovered in the animal reservoir of H7N7 or H9N2, paralleling the potential donor pools for 188T and 454N onto the human pandemic.

Declaration of Pandemic conclusion via press release has not effectively prevented emergent behaviour in this zoonoticly-active disease reservoir. Influenza Flux is apparently ongoing as the world remains in a pre-PF11<SUB>Ω</SUB> phase.

An enterprising question might be, ?How many Hydra strains of Vaccine Escape capacity will co-circulate in this Northern Hemisphere winter??


. . . . ChinaJiangsuNanjing1_2010_06_30 (
. . . . . . . . 131P,
. . . . . . . . 158E [H3N8, H5N1],
. . . . . . . . 225G,
. . . . . . . . 454G [Unique to PF11 Jiangsu],
. . . . . . . . 488N [H3N8 2009, H6, H9N2, NewJersey01_2010_01_01])

. . . . ChinaJiangsuNanjing2_2010_06_30 (
. . . . . . . . 131P,
. . . . . . . . 158E [H3N8, H5N1],
. . . . . . . . 225G,
. . . . . . . . 454G [Unique to PF11 Jiangsu],
. . . . . . . . 488N [H3N8 2009, H6, H9N2, NewJersey01_2010_01_01])

. . . . ChinaJiangsu1_2009_11_20_re (
. . . . . . . . 131P,
. . . . . . . . 158E [H3N8, H5N1],
. . . . . . . . 225G,
. . . . . . . . 454G [Unique to PF11 Jiangsu],
. . . . . . . . 488N [H3N8 2009, H6, H9N2, NewJersey01_2010_01_01])

. . . . ChinaJiangsu2_2009_11_13_re (
. . . . . . . . 131P,
. . . . . . . . 158E [H3N8, H5N1],
. . . . . . . . 225G,
. . . . . . . . 454G [Unique to PF11 Jiangsu],
. . . . . . . . 488N [H3N8 2009, H6, H9N2, NewJersey01_2010_01_01])

. . . . ChinaJiangsuS61_2009_11_10 (
. . . . . . . . 54R,
. . . . . . . . 84G [Unique to GenBank PF11],
. . . . . . . . 131P,
. . . . . . . . 158E [H3N8, H5N1],
. . . . . . . . 211R [swOR4060_2009_12_31],
. . . . . . . . 225G,
. . . . . . . . syn319T [MississippiAF2473_2010_03_04 with 60T, 225E,
. . . . . . . . . . . . . . . FloridaAF2199_2010_03_11 with 60T, 225E,
. . . . . . . . . . . . . . . YaroslavlIIV196_2009_12_04_f with 225G
. . . . . . . . . . . . . . . SpainCatS1236_2009_08_13 with 225E,
. . . . . . . . . . . . . . . Finland612_2009_07_21 with 225E,
. . . . . . . . . . . . . . . KazAstana818_2009_07,
. . . . . . . . . . . . . . . KazAlmati01_2009 with 225E],
. . . . . . . . 454G [Unique to PF11 Jiangsu],
. . . . . . . . 488N [H3N8 2009, H6, H9N2, NewJersey01_2010_01_01])

. . . . ChinaJiangsuS62_2009_11_10 (
. . . . . . . . 60T [MississippiAF2473_2010_03_04 with 225E, syn319T,
. . . . . . . . . . . FloridaAF2199_2010_03_11 with 225E, syn319T],
. . . . . . . . 131P,
. . . . . . . . 158E [H3N8, H5N1],
. . . . . . . . 225G,
. . . . . . . . 454G [Unique to PF11 Jiangsu],
. . . . . . . . 481G,
. . . . . . . . 488N [H3N8 2009, H6, H9N2, NewJersey01_2010_01_01])

Supporting Sequences

. . . . RussiaBelgorod2_2010_03_15 (
. . . . . . . . syn13N [WiscD0459_2009_12_18_xL
. . . . . . . . . . . . . . . . . . with 189T, 324I & 499K,
. . . . . . . . . . . . . . LouisianaAF2435_2009_11_30
. . . . . . . . . . . . . . . . . . with syn338G],
. . . . . . . . 77N [tn, swine, S5]
. . . . . . . . . . . [JapanOsaka60_2010_07_02
. . . . . . . . . . . . . . . . with syn276H,
. . . . . . . . . . . Maryland04_2010_02_08
. . . . . . . . . . . . . . . . with syn276H,
. . . . . . . . . . . ShandongShizhongSWL24_2010_01_24,
. . . . . . . . . . . NY7020_2009_12_14
. . . . . . . . . . . . . . . . with syn166K & 189T,
. . . . . . . . . . . CalifVRDL115_2009_12_04
. . . . . . . . . . . . . . . . with syn276H,
. . . . . . . . . . . JapanAF2267_2009_11_09
. . . . . . . . . . . . . . . . with 286E,
. . . . . . . . . . . NevadaAF2490_2009_11_02
. . . . . . . . . . . . . . . . with 100N & syn270I,
. . . . . . . . . . . AlaskaAF2093_2009_11_02
. . . . . . . . . . . . . . . . with 286E,
. . . . . . . . . . . Russia74_2009_11_01_xL,
. . . . . . . . . . . ThailandTHB0441_2009_07_28,
. . . . . . . . . . . Lisboa40_2009_06_23,
. . . . . . . . . . . AnhuiSWL1_2009_06_18
. . . . . . . . . . . . . . . . with 275I,
. . . . . . . . . . . Lithuania1942_2009,
. . . . . . . . . . . swKoreaSCJ10_2009_12,
. . . . . . . . . . . swKoreaSCJ09_2009_12]
. . . . . . . . 158E mix,
. . . . . . . . 225G (Gga) stepwise from 225E,
. . . . . . . . 230I (ATa),
. . . . . . . . 238D [H2N3, H7N3, H7N7, tn, swine],
. . . . . . . . . . . . [PNG_Goroka17_2010_04_14,
. . . . . . . . . . . . AthensINS398_2010_01_24,
. . . . . . . . . . . . AthensINS339_2009_12_30,
. . . . . . . . . . . . Kaliningrad01_11M_2009_11_02
. . . . . . . . . . . . . . . . . . with 225E & 226R,
. . . . . . . . . . . . KaliningradCRIE_DA_2009_09_26,
. . . . . . . . . . . . KaliningradCRIE_KG_2009_09_25,
. . . . . . . . . . . . KaliningradCRIE_SHD_2009_09_25,
. . . . . . . . . . . . KaliningradCRIE_MA_2009_09_25,
. . . . . . . . . . . . KaliningradCRIE_ZD_2009_09_25],
. . . . . . . . syn245F [H2N3, H5N1, H7N7, H10N7, H11]
. . . . . . . . . . . . . . [SingON2416_2009_12_15
. . . . . . . . . . . . . . . . . . . . . with syn166K & 300P,
. . . . . . . . . . . . . . . EgyptAswan2288_2009_11_02
. . . . . . . . . . . . . . . . . . . . . with 189T],
. . . . . . . . 300S [H7N3, H7N7 (tCt)],
. . . . . . . . 305N (AAc) [ThaiChiangRai226_2M_2010_03_04
. . . . . . . . . . . . . . . . . . . . . . . with 270T,
. . . . . . . . . . . . . . . . . . Brunei5_52M_2010,
. . . . . . . . . . . . . . . . . . SouthCarolinaAF2562_2009_11_15 (AAt),
. . . . . . . . . . . . . . . . . . DenmarkAalborgINS133_2009_12_02 (AAt)],
. . . . . . . . syn343G [H3N8, H5N1, H6N1, H7N3, H7N7, H10N7, H11, 1918],
. . . . . . . . . . . . . . . [Thuringen189_2009_11_24_xL,
. . . . . . . . . . . . . . . Thuringen227_11_17_xL])

. . . . Brisbane209_51F_2010_08_09 (
. . . . . . . . 156E,
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . 225G,
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . . . . . . . . [Victoria670_30M_2010_11_14
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . Emergent across Australia
. . . . . . . . . . . . . . . . . . . . . during late 2010 season,
. . . . . . . . . . . . . . . Florida14_24M_2010_08_05
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . OZVictoria512_2010_07_30
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . NZChristchurch15_2010_07_12
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . India5107_2010_06_28
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . MississippiAF2474_2010_03_10
. . . . . . . . . . . . . . . . . . . . . with syn235T,
. . . . . . . . . . . . . . . FloridaAF2197_2010_03_07
. . . . . . . . . . . . . . . . . . . . . with 156T,
. . . . . . . . . . . . . . . CalifVRDL9_2010_02_09
. . . . . . . . . . . . . . . . . . . . . with syn193S,
. . . . . . . . . . . . . . . Georgia06_2010_02_05
. . . . . . . . . . . . . . . . . . . . . with syn161Y,
. . . . . . . . . . . . . . . NY4662_2010_02_03
. . . . . . . . . . . . . . . . . . . . . with 97N, syn276H, syn283Q, syn304G,
. . . . . . . . . . . . . . . TexasJMS406_2010_01_10
. . . . . . . . . . . . . . . . . . . . . with 187A, syn193S, syn283Q,
. . . . . . . . . . . . . . . TexasJMS405_2010_01_09
. . . . . . . . . . . . . . . . . . . . . with 187A, syn283Q,
. . . . . . . . . . . . . . . CalifVRDL131_2009_12_30
. . . . . . . . . . . . . . . . . . . . . with 225G, syn455Q,
. . . . . . . . . . . . . . . LouisianaAF2435_2009_11_30
. . . . . . . . . . . . . . . . . . . . . with syn13N,
. . . . . . . . . . . . . . . Vienna291_2009_11_19
. . . . . . . . . . . . . . . catOregon29573_2009_11_09
. . . . . . . . . . . . . . . . . . . . . with 226R, syn283Q,
. . . . . . . . . . . . . . . Calif_SanDiegoINS63_2009_10_26
. . . . . . . . . . . . . . . . . . . . . with syn283Q, et al],
. . . . . . . . 377K,
. . . . . . . . 454N [H7N3, H7N7, H9N2]
. . . . . . . . . . [Florida14_24M_2010_08_05
. . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . FL_Pen210_2009_11_10
. . . . . . . . . . . . . . . . . . with 225E,
. . . . . . . . . . SouthCarolina18_2009_09_16_VxX
. . . . . . . . . . . . . . . . . . with 159D, 224K,
. . . . . . . . . . Texas45131774_2009_09_13
. . . . . . . . . . . . . . . . . . with syn223V,
. . . . . . . . . . IndiaPune9355_2009_08
. . . . . . . . . . . . . . . . . . with 225G,
. . . . . . . . . . IndiaBlore236_2009_06_xL
. . . . . . . . . . . . . . . . . . with 226R, et al],
. . . . . . . . syn465N)

[NS1]

HA 225G Paired with 156E &amp; 188T in Diverse Deposit from Australia

HA 225G Paired with 156E & 188T in Diverse Deposit from Australia

http://pf11.blogspot.com/2010/12/ha-225g-paired-with-188t-in-diverse.html

Last Updated 2010-12-16

A WHO Collaborating Centre for Reference and Research on Influenza released a large group of sequences at GISAID on 2010-12-14 primarily from Australia. 225G appears on two different backgrounds during the same timeframe, one with the H7N7 polymorphism that has been associated with viral conjunctivitis, 188T. OzBrisbane209_51F_2010_08_09 also demonstrates a 156E polymorphism just upstream from the section at aa158 and aa159 that has produced so many "Low Reactor" / Vaccine Escape strains.

156E was found in the 1976 Swine Flu with 225G, but until now has not been documented in the pH1N1 with 225G, though the individual change has been located in 9 sequences [New Zealand, Japan (2), China 2010, Hungary, Germany (2) and the United States (2)]. Changes at residues 156, 157 and 158 are known to increase viral replication speed and success on the pandemic H1N1 background. At the MedImmune growth speed experiments preparing for the 2009 Live Attenuated Influenza Vaccine, the combination of 156E and 225G created the highest pathological output (V5-153E) (<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2798434/" target="_blank">J Virol. 2010 January; 84(1): 44?51</a>). Though the V5-153E strain produced the most beneficial viral output, the lineage was impeached due to a 16 fold titers reduction in the antigenicity tests indicating a profound level of "Low Reactor".

This Brisbane sample has not yet been tagged for Vaccine Escape.

As a set of coincident events, several zoonotic Influenza reservoirs have also gained 156E in recent years. This group of reservoirs appears to parallel individual changes coming into the human H1N1 pandemic. H5N1 human cases in Vietnam carry this change from 2004. The most current updates of Avian sequences from Egypt H5N1 in 2009 up to July 2010 demonstrate emergent 156E with 225G (Ggg). One particular Egyptian bird, ckEgypt10135ss_2010_03, shows 156E, 225G and a variant coding for syn338G (GGg).

H3N8 equine and swine samples are on record producing this emergent change. H7N3 and H7N7 each provide material for nucleotide donation at the first codon base while an extensive percentage of the H10N7 sequences on file carry the 156E. A hybrid swine H1N1 sequence from Iowa,
swIowa44837_1_2009_11_08_xL, was recently published after a one year lag with 156E, 188R, 225N, 230I & syn346G.

This Brisbane strain is emergent and aggregates 225G with markers found throughout the coastal United States during late 2009 and 2010. On a similar note, a sub-clade may perhaps be forming from Jiangsu Province, China using 225G paired with 158E and a change at amino acid position 454.

The 215T found in the sequence from the 9 year old boy, Sydney202_9M_2010_07_18, confirms an increase in the genetic diversity of the post-pandemic reservoir found during the official pandemic on an eastern European sequence, PolandWarsawINS316_2009_12_08.

As we have previously reported, 100% of the Hemagglutinin positions between 186 and 248, including antigenic areas of the Receptor Binding Domain (RBD/RBS), are on record as polymorphic. No position in that range is stable. Many positions rate multiple changes. Protein revision is documented at 60 of the 63 positions (95%), engaging the potential for antibody resistance (natural immune escape and vaccine escape).

. . . . OzBrisbane209_51F_2010_08_09 (
. . . . . . . . 156E [H2N3, H3N8, H5N1, H10N7],
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . 225G,
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . . . . . . . . [OzVictoria670_30M_2010_11_14
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . Emergent across Australia
. . . . . . . . . . . . . . . . . . . . . during late 2010 season,
. . . . . . . . . . . . . . . Florida14_24M_2010_08_05
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . OZVictoria512_2010_07_30
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . NZChristchurch15_2010_07_12
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . India5107_2010_06_28
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . MississippiAF2474_2010_03_10
. . . . . . . . . . . . . . . . . . . . . with syn235T,
. . . . . . . . . . . . . . . FloridaAF2197_2010_03_07
. . . . . . . . . . . . . . . . . . . . . with 156T,
. . . . . . . . . . . . . . . CalifVRDL9_2010_02_09
. . . . . . . . . . . . . . . . . . . . . with syn193S,
. . . . . . . . . . . . . . . Georgia06_2010_02_05
. . . . . . . . . . . . . . . . . . . . . with syn161Y,
. . . . . . . . . . . . . . . NY4662_2010_02_03
. . . . . . . . . . . . . . . . . . . . . with 97N, syn276H, syn283Q, syn304G,
. . . . . . . . . . . . . . . TexasJMS406_2010_01_10
. . . . . . . . . . . . . . . . . . . . . with 187A, syn193S, syn283Q,
. . . . . . . . . . . . . . . TexasJMS405_2010_01_09
. . . . . . . . . . . . . . . . . . . . . with 187A, syn283Q,
. . . . . . . . . . . . . . . CalifVRDL131_2009_12_30
. . . . . . . . . . . . . . . . . . . . . with 225G, syn455Q,
. . . . . . . . . . . . . . . LouisianaAF2435_2009_11_30
. . . . . . . . . . . . . . . . . . . . . with syn13N,
. . . . . . . . . . . . . . . Vienna291_2009_11_19
. . . . . . . . . . . . . . . catOregon29573_2009_11_09
. . . . . . . . . . . . . . . . . . . . . with 226R, syn283Q,
. . . . . . . . . . . . . . . Calif_SanDiegoINS63_2009_10_26
. . . . . . . . . . . . . . . . . . . . . with syn283Q, et al],
. . . . . . . . 377K,
. . . . . . . . 454N [H7N3, H7N7, H9N2]
. . . . . . . . . . [Florida14_24M_2010_08_05
. . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . FL_Pen210_2009_11_10
. . . . . . . . . . . . . . . . . . with 225E,
. . . . . . . . . . SouthCarolina18_2009_09_16_VxX
. . . . . . . . . . . . . . . . . . with 159D, 224K,
. . . . . . . . . . Texas45131774_2009_09_13
. . . . . . . . . . . . . . . . . . with syn223V,
. . . . . . . . . . IndiaPune9355_2009_08
. . . . . . . . . . . . . . . . . . with 225G,
. . . . . . . . . . IndiaBlore236_2009_06_xL
. . . . . . . . . . . . . . . . . . with 226R, et al],
. . . . . . . . syn465N)

. . . . Sydney202_9M_2010_07_18 (
. . . . . . . . 34D,
. . . . . . . . syn44L,
. . . . . . . . 97N,
. . . . . . . . syn106E,
. . . . . . . . 128D,
. . . . . . . . 215T [PolandWarsawINS316_2009_12_08]
. . . . . . . . 225G,
. . . . . . . . 253A,
. . . . . . . . syn362S,
. . . . . . . . 377K,
. . . . . . . . 439G [Guangdong5024_2009_10_20])

Supporting Sequences

. . . . Florida14_24M_2010_08_05 (
. . . . . . . . 100N [H7N3, H7N7, H9N2],
. . . . . . . . syn161Y [H3N8 2009, H6N1, H9N2],
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . 377K [H9N2],
. . . . . . . . 442I mix,
. . . . . . . . 454N [H7N3, H7N7, H9N2])

. . . . OZVictoria512_2010_07_30 (
. . . . . . . . syn60I,
. . . . . . . . 100N [H7N3, H7N7, H9N2],
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . syn285P,
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . 377K [H9N2],
. . . . . . . . 454N [H7N3, H7N7, H9N2])

. . . . Thailand34_9912_2010_07_14 (
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . 200T,
. . . . . . . . HA truncated after aa253)

. . . . Thailand34_9937_2010_07_14 (
. . . . . . . . 162Q [Unique to PF11 GenBank/GISAID],
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . HA truncated after aa253)

. . . . NZChristchurch15_2010_07_12 (
. . . . . . . . 100N,
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . 377K [H9N2],
. . . . . . . . 454N [H7N3, H7N7, H9N2])

. . . . India5107_2010_06_28 (
. . . . . . . . syn60I,
. . . . . . . . 100N [H7N3, H7N7, H9N2],
. . . . . . . . syn135V,
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . 377K [H9N2],
. . . . . . . . 454N [H7N3, H7N7, H9N2])

. . . . India8910_2010_05_08 (
. . . . . . . . syn49G [H7N3, H7N7],
. . . . . . . . syn51A,
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . 377K [H9N2],
. . . . . . . . 454N [H7N3, H7N7, H9N2])

. . . . swThaiCURA75_2010_01 (
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . 263D,
. . . . . . . . syn350G [H7N3, H7N7],
. . . . . . . . 414I,
. . . . . . . . syn484N [H3N8, H4, H5N1 (E/A/H), H6, H7N7, H9N2, H11])

. . . . Texas45131774_2009_09_13 (
. . . . . . . . syn223V [H5N1, H6N1],
. . . . . . . . 454N [H7N3, H7N7, H9N2]
. . . . . . . . . . . . . . [IndiaPune9355_2009_08 with 225G,
. . . . . . . . . . . . . . IndiaBlore236_2009_06 with 226R,
. . . . . . . . . . . . . . SouthCarolina18_2009_09_16_VxX with 224K,
. . . . . . . . . . . . . . FL_Pen210_2009_11_10 with 225E])

. . . . PolandWarsawINS316_2009_12_08 (
. . . . . . . . 22I,
. . . . . . . . syn92T,
. . . . . . . . 119M,
. . . . . . . . 165N,
. . . . . . . . syn178V,
. . . . . . . . syn186S,
. . . . . . . . 215T,
. . . . . . . . syn256Y,
. . . . . . . . syn275V,
. . . . . . . . 463V)

[Giuseppe]

Re: 225G Worldwide Tracking &amp; Evaluation

Source: Eurosurveillance: http://www.eurosurveillance.org/View...rticleId=19760 also at FT.

Images URL:

(1) Influenza A H1N1 (2009) phylogenetic tree, http://www.eurosurveillance.org/imag...IS_Fig3new.jpg
(2) Influenza B, phylogenetic tree, http://www.eurosurveillance.org/imag...LLIS_Fig4-.jpg

[1]


[2]


-
------

[NS1]

UK Fatality Sequences Relate to US and Iran

UK Fatality Sequences Relate to US and Iran

The <strong><span style="color: #783f04;">US CDC</span></strong> today released a small set of sequences at GISAID spanning June to November 2010. As the UK Severe Wave is the current topic of interest,&nbsp;we have profiled 6 of these sequences from today against the UK Fatality Sequences that have been published.&nbsp; Five of the sequences are <strong><span style="color: #783f04;">from the United States</span></strong> and one is from Bangladesh.

Previously, the UK HPA released a small group of sequences in two deposits at GISAID related to the present severe wave filling the ICU wards&nbsp;in the UK. A concurrent paper ^{<strong><span style="color: red;">1</span></strong>} was published in early January with a phylogenetic tree. <strong><span style="color: #783f04;">Several of the fatalities</span></strong> noted on the HPA Ellis Figure3 ^{<strong><span style="color: red;">1</span></strong>} phylogenetic tree have been included in the two sequence deposits.

So that focus may occur at the clinical outcomes of highest priority, <strong><span style="color: #783f04;">GeneWurx</span></strong> has prepared a fresh <a href="http://genewurx.com/data/GeneWurx_Global_Spread_of_Divergence_UK_US_Iran_v0 .JPG" target="_blank">phylogenetic tree</a> with annotation in progress that may prove useful to those investigating the <strong><span style="color: #783f04;">ongoing divergency</span></strong> within this <strong><span style="color: #660000;">zoonoticly active</span></strong> <strong><span style="color: #4c1130;">pH1N1</span></strong> <a href="http://pf11.blogspot.com/2011/01/uk-and-iran-transmit-hyper-zoonotic.html" target="_blank">viral reservoir</a>.&nbsp; UK Fatalities, UK Severe cases (<strong><span style="color: blue;">225G</span></strong>), UK potential Vaccine Escape cases (<strong><span style="color: blue;">158E, 159K</span></strong>)&nbsp;and cases from the&nbsp;US, Australia, Iran and&nbsp;Bangladesh are profiled.

<strong><a href="http://genewurx.com/data/GeneWurx_Global_Spread_of_Divergence_UK_US_Iran_v0 .JPG" target="_blank">GeneWurx_Global_Spread_of_Divergen ce_UK_US_Iran_v0.jpg</a></strong>

The <strong><span style="color: #783f04;">GeneWurx</span></strong> annotation&nbsp;for the full HPA Ellis Figure3 ^{<strong><span style="color: red;">1</span></strong>} has been recently revised and&nbsp;<a href="http://genewurx.com/data/GeneWurx_UK_December_Emerging_Genetics_v5.xls" target="_blank">Version 5</a> of the Emerging Genetics spreadsheet is available with several additions including two of the recent US sequences of interest and the Bangladeshi sequence.
<ul>
<li><a href="http://genewurx.com/data/UK_2010_Phylo_ELLIS_Fig3new_4_GISAID_Notated_2011_ 01_13.JPG" target="_blank">UK_2010_Phylo_ELLIS_Fig3new_4_GISA ID_Notated_2011_01_13.JPG</a></li>
<li><a href="http://genewurx.com/data/GeneWurx_UK_December_Emerging_Genetics_v5.xls" target="_blank">GeneWurx_UK_December_Emerging_Gene tics_v5.xls</a></li>
</ul>
A pattern that has been expected is demonstrated in <strong><span style="color: #783f04;">Kentucky09_40F_2010_11_01</span></strong> with <strong><span style="color: blue;">158E, 188T</span></strong> and <strong><span style="color: blue;">225G</span></strong> on a similar background as the <strong><span style="color: #783f04;">OzBrisbane209_51F_2010_08_09</span></strong> <a href="http://pf11.blogspot.com/2010/12/ha-225g-paired-with-188t-in-diverse.html">sequence</a> carrying <strong><span style="color: blue;">156E, 188T</span></strong> and <strong><span style="color: blue;">225G</span></strong>.&nbsp; Though these two sequences share extensive homology, the tree and polymorphism details will show that <strong><span style="color: #783f04;">overall the reservoir is diversifying</span></strong> and creating <strong><span style="color: #660000;">branches with minimal leaf count</span></strong>&nbsp;by acquiring and recombining <strong><span style="color: #660000;">rare and novel</span></strong> polymorphisms also found in <strong><span style="color: #660000;">animal reservoirs</span></strong>.

<span style="color: #274e13; font-size: x-large;">US Sequences</span>

. . . . Indiana05_78F_2010_06_11 (
. . . . . . . . 100N,
. . . . . . . . 115K,
. . . . . . . . syn270I,
. . . . . . . . 377K)

. . . . Indiana06_9M_2010_07_29 (
. . . . . . . . #11V,
. . . . . . . . #8A,
. . . . . . . . syn36L,
. . . . . . . . syn99I,
. . . . . . . . 233H,
. . . . . . . . syn256Y,
. . . . . . . . syn282C,
. . . . . . . . syn283Q,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . syn428L,
. . . . . . . . 522A)

. . . . Kentucky08_xF_2010_10_11 (
. . . . . . . . 100N,
. . . . . . . . syn179L,
. . . . . . . . 188T,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . syn448L,
. . . . . . . . 454N)

. . . . Utah05_29F_2010_10_12 (
. . . . . . . . 100N,
. . . . . . . . 188T,
. . . . . . . . 289M,
. . . . . . . . syn297N,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . 396I,
. . . . . . . . 454N,
. . . . . . . . syn478C)

. . . . Kentucky09_40F_2010_11_01 (
. . . . . . . . 17G,
. . . . . . . . 100N,
. . . . . . . . <strong><span style="color: blue;">158E</span></strong>,
. . . . . . . . syn179L,
. . . . . . . . <strong><span style="color: blue;">188T</span></strong>,
. . . . . . . . <strong><span style="color: blue;">225G</span></strong>,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . syn448L,
. . . . . . . . syn452V,
. . . . . . . . 454N,
. . . . . . . . syn467C)

<span style="color: #274e13; font-size: x-large;">Bangladesh Sequence</span>

. . . . Bangladesh8003_27M_2010_09_16 (
. . . . . . . . syn12A,
. . . . . . . . 137T,
. . . . . . . . 186P,
. . . . . . . . 225N mix wt,
. . . . . . . . syn297N,
. . . . . . . . syn326S,
. . . . . . . . syn383N,
. . . . . . . . syn388K,
. . . . . . . . 444K,
. . . . . . . . 447S,
. . . . . . . . syn474C)

<span style="color: #274e13; font-size: x-large;">UK Fatality Sequences</span>

. . . . UKEngland5040499_2010_12_f (
[match GhanaFS10_4259_2010_08_27 less 213S]
. . . . . . . . 100N,
. . . . . . . . 188T,
. . . . . . . . 213S,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . 454N,
. . . . . . . . syn537S)

. . . . UKWhiteChapel4880374_2M_2010_11_28_f (
. . . . . . . . 0A [Arizona05_2010_05_01
. . . . . . . . . . . . . . . with syn338G &amp; 377K,
. . . . . . . . . . Alabama08_2009_12_04
. . . . . . . . . . . . . . . with syn226Q, 310A mix &amp; 506V,
. . . . . . . . . . RussiaPerm_ZTS_2009_11_30
. . . . . . . . . . . . . . . with 377K &amp; syn475D,
. . . . . . . . . . RussiaBelgorod01_2009_11_30,
. . . . . . . . . . RussiaBelgorod05_2009_11_30,
. . . . . . . . . . Boston634_2009_11_09
. . . . . . . . . . . . . . . with 100N &amp; syn270I],
. . . . . . . . syn55L [S9, H5N1],
. . . . . . . . . . . . . . [Iran16273_2009_11_22 with 226R
. . . . . . . . . . . . . . NZ_Waikato2_2010_01_04 with 233H,
. . . . . . . . . . . . . . tkOntarioFAV117_1C_2009_12_07, et al],
. . . . . . . . 188T [H6N1, H7N7],
. . . . . . . . syn338G [H3N8, H4, H5, H6, sw],
. . . . . . . . . . . . . . . [OzBrisbane209_51F_2010_08_09
. . . . . . . . . . . . . . . . . . . . with 156E &amp; 225G,
. . . . . . . . . . . . . . . Arizona05_2010_05_11 with 0A,
. . . . . . . . . . . . . . . Swine Asia 2005 with 0A, et al]
. . . . . . . . 377K,
. . . . . . . . 454N [H7N3, H7N7, H9N2]
. . . . . . . . . . [Florida14_24M_2010_08_05
. . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . FL_Pen210_2009_11_10
. . . . . . . . . . . . . . . . . . with 225E,
. . . . . . . . . . SouthCarolina18_2009_09_16_VxX
. . . . . . . . . . . . . . . . . . with 159D, 224K, et al],
. . . . . . . . syn529L)

. . . . UKCambridge118_4F_2010_11_19_f (
. . . . . . . . syn118E,
. . . . . . . . 137T [41 sequences at GISAID],
. . . . . . . . syn163K,
. . . . . . . . 186P,
. . . . . . . . syn251L,
. . . . . . . . syn293L,
. . . . . . . . syn363G [21 sequences at GISAID],
. . . . . . . . syn455Q,
. . . . . . . . syn474C,
. . . . . . . . 504G,
. . . . . . . . 513V)

. . . . UKEngland4500186_2010_11_f (
. . . . . . . . 137T (aCA) [H3N8 donor aAT, aGT, aGC],
. . . . . . . . 186P [Avian H12 2008, 2009], [Avian H1N1],
. . . . . . . . 190Y [Avian H6N1],
. . . . . . . . . . [SwedenMalmoe1_2010_01_01_xL
. . . . . . . . . . . . with 377K, syn413K,
. . . . . . . . . . Brasil7450_2009_07_22
. . . . . . . . . . . . with 157E mix wt],
. . . . . . . . 377G [H2N3],
. . . . . . . . . . . . . [H5N1 Human Egypt Preschool 2009, H5N1 Human Indonesia 2005],
. . . . . . . . . . . . . [H6N1 2009],
. . . . . . . . . . . . . [US Swine 1957-1978],
. . . . . . . . . . . . . [IranBandarAbbas5096_2010_10_02
. . . . . . . . . . . . . . . . . . . with 137T, 186P, syn474C,
. . . . . . . . . . . . . Florida13_2010_08_02
. . . . . . . . . . . . . . . . . . . with 137T, 186P, syn474C, 512M,
. . . . . . . . . . . . . . Iowa05_2010_05_04
. . . . . . . . . . . . . . . . . . . with 208K, syn516I,
. . . . . . . . . . . . . . swSouthDakota1_2010_04_27,
. . . . . . . . . . . . . . TexasAF2647_2010_04_11
. . . . . . . . . . . . . . . . . . . with 200S, syn214K, syn276H,
. . . . . . . . . . . . . . swIowa02999_2010_04_01,
. . . . . . . . . . . . . . UtahAF2653_2010_03_14
. . . . . . . . . . . . . . . . . . . with syn253V,
. . . . . . . . . . . . . . North Carolina05_2010_02_24
. . . . . . . . . . . . . . . . . . . with 259V,
. . . . . . . . . . . . . . Kentucky04_2010_02_22
. . . . . . . . . . . . . . . . . . . with syn488D,
. . . . . . . . . . . . . . Minnesota02_2010_02_17
. . . . . . . . . . . . . . . . . . . with 259V, 509D,
. . . . . . . . . . . . . . SouthCarolina01_2010_01_27
. . . . . . . . . . . . . . . . . . . with syn238E, syn488D,
. . . . . . . . . . . . . . AnasPlatBelgium04328_pcs17_2010_01_21
. . . . . . . . . . . . . . . . . . . with syn474C,
. . . . . . . . . . . . . . GermanyAF2208_2010_01_20
. . . . . . . . . . . . . . . . . . . with #1T, 225E,
. . . . . . . . . . . . . . NorthCarolina03_2010_01_20
. . . . . . . . . . . . . . . . . . . with 225G mix, 259V, syn275V,
. . . . . . . . . . . . . . NewMexico01_2010_01_05
. . . . . . . . . . . . . . . . . . . with 430I, syn445V, syn490P,
. . . . . . . . . . . . . . swThailandCU_CHK4_2009_01
. . . . . . . . . . . . . . . . . . with #1T, 0A, 189T, syn338G,
. . . . . . . . . . . . . . . . . . . . . . syn451K, syn474C, syn456L, syn529L,
. . . . . . . . . . . . . . Moldova5053_2009_12_11
. . . . . . . . . . . . . . . . . . with 173R, 176I, 225A, 225G mix wt, syn286K,
. . . . . . . . . . . . . . WiscD0128_2009_11_15
. . . . . . . . . . . . . . . . . . . with syn297N, syn343G, 471H,
. . . . . . . . . . . . . . KoreaAF2411_2009_11_11
. . . . . . . . . . . . . . . . . . . with 237I, syn286K,
. . . . . . . . . . . . . . England94840077_2009_11
. . . . . . . . . . . . . . . . . . . with 259T,
. . . . . . . . . . . . . . BahrainN11892_2009_10
. . . . . . . . . . . . . . . . . . . with 208K,
. . . . . . . . . . . . . . Nevada16_2009_10_02
. . . . . . . . . . . . . . . . . . . with 464R mix wt,
. . . . . . . . . . . . . . Tasmania2005_2009_07_03
. . . . . . . . . . . . . . . . . . . with 289M,
. . . . . . . . . . . . . . Texas42303371_2009
. . . . . . . . . . . . . . . . . . . with syn456L, syn507K],
. . . . . . . . syn408E,
. . . . . . . . syn474C,
. . . . . . . . 512M,
. . . . . . . . 530I)

. . . . UKEngland4640543_2010_11_f (
[match to UKEngland4940476_2010_12 less 225G plus 190G]
. . . . . . . . 100N,
. . . . . . . . syn179L,
. . . . . . . . 188T,
. . . . . . . . 190G,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . syn448L,
. . . . . . . . 454N)

. . . . UKWhiteChapel4780352_5M_2010_10_26_f (
. . . . . . . . syn58C,
. . . . . . . . 100N,
. . . . . . . . 128D,
. . . . . . . . syn131S,
. . . . . . . . syn210S,
. . . . . . . . 377K)

. . . . UKEngland4380108_2010_10_f (
. . . . . . . . syn67N,
. . . . . . . . 100N,
. . . . . . . . 128D,
. . . . . . . . 144V [Boston703 and Ireland],
. . . . . . . . 224K,
. . . . . . . . syn235T,
. . . . . . . . 377K,
. . . . . . . . 529M)

. . . . UKBirmingham3220137_44F_2010_08_07_f (
. . . . . . . . #8A,
. . . . . . . . 175K,
. . . . . . . . 311E,
. . . . . . . . 377K,
. . . . . . . . syn385V,
. . . . . . . . syn451K,
. . . . . . . . syn454S,
. . . . . . . . syn494E,
. . . . . . . . 537G)

<span style="color: #274e13; font-size: x-large;">Severe Sequences</span>

. . . . UKEngland4940476_2010_12 (
. . . . . . . . 100N,
. . . . . . . . syn179L (CTg) [Regional Marker 2009 (tTA)]
. . . . . . . . . . . . . . . . . . . . [TexasAF2588_2009_10_04,
. . . . . . . . . . . . . . . . . . . . TexasJMS404_2010_01_08],
. . . . . . . . 188T,
. . . . . . . . 225G,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . syn448L [H6N1]
. . . . . . . . . . . . . . . [Regional Marker UK 2009]
. . . . . . . . . . . . . . . [UKEngland4880378_2010_12 with syn179L, 188T,
. . . . . . . . . . . . . . . UKEngland4640543_2010_11_f with syn179L, 188T, 190G
. . . . . . . . . . . . . . . UKEngland4920303_2010_11 with syn179L, 188T,
. . . . . . . . . . . . . . . UKEngland142_2010_11 with syn179L, 188T,
. . . . . . . . . . . . . . . UKEngland4860049_2010_11 with syn179L, 188T,
. . . . . . . . . . . . . . . UKEngland126_2010_11 with syn179L, 188T,
. . . . . . . . . . . . . . . NZChristchurch8_2010_07_08
. . . . . . . . . . . . . . . . . . . with syn44L, 97N, syn99I, syn106E, 128D,
. . . . . . . . . . . . . . . . . . . . . . . syn214K, 253A, syn362S, 377K,
. . . . . . . . . . . . . . . Calif06_2010_04_05 with 269V
. . . . . . . . . . . . . . . Hawaii08_2010_04_12
. . . . . . . . . . . . . . . . . . . with 159D, 269V, 312R, 313K,
. . . . . . . . . . . . . . . Ethiopia13_2010_02_10
. . . . . . . . . . . . . . . . . . . with 100N, syn163K, 269V, 324I, syn360Q, syn455Q,
. . . . . . . . . . . . . . . Philippines824_2010_02_17 with 165N,
. . . . . . . . . . . . . . . Kosova876_2009_12_22,
. . . . . . . . . . . . . . . RussiaYakutsk_EAV_2009_11_18,
. . . . . . . . . . . . . . . Netherlands2143_2009_11_16 with syn179L (tTA),
. . . . . . . . . . . . . . . RussiaYaroslavl_CHMV_2009_11_10_f with 224K &amp; 225G mix
. . . . . . . . . . . . . . . AntwerpINS221_2009_10_28 with syn179L (tTA)],
. . . . . . . . 454N)

. . . . UKEngland4880378_2010_12 (
. . . . . . . . 100N,
. . . . . . . . syn179L,
. . . . . . . . 188T,
. . . . . . . . 225G,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . syn448L,
. . . . . . . . 454N)

<span style="color: #274e13; font-size: x-large;">Potential Vaccine Escape Sequences</span>

[<a href="http://pf11.blogspot.com/2011/01/uk-severe-wave-demonstrates-diversity.html" target="_blank">Diversity with Vaccine Escape Potential</a>]

. . . . UKEngland106_2010_11 (
. . . . . . . . 0A,
. . . . . . . . syn55L,
. . . . . . . . 158E mix wt,
. . . . . . . . 188T,
. . . . . . . . syn258F,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . 452I [],
. . . . . . . . 454N,
. . . . . . . . syn529L)

. . . . UKEngland3380426_2010_08 (
. . . . . . . . syn44L,
. . . . . . . . syn106E,
. . . . . . . . 128D,
. . . . . . . . 159K [NY3230_2010_01_25 with 100N, syn177L, syn231N],
. . . . . . . . 377K)

<span style="color: #274e13; font-size: x-large;">Iran Sequences</span>

. . . . IranShahriar5336_2010_12_06 (
. . . . . . . . 137T (aCA) [H3N8 donor aAT, aGT, aGC],
. . . . . . . . 186P [Avian H12 2008, 2009], [Avian H1N1],
. . . . . . . . syn297N [H3N8 gadwallRussiaAltai1325_2007_09],
. . . . . . . . . . . . . . . [H5N1],
. . . . . . . . . . . . . . . [Wisconsin08_2010_08_10
. . . . . . . . . . . . . . . . . . . . . . with 39R, syn78S, 137T, 186P, syn297N,
. . . . . . . . . . . . . . . . . . . . . . . . . . syn326S, syn346G, syn388K,
. . . . . . . . . . . . . . . . . . . . . . . . . . syn413K, 444K, syn474C,
. . . . . . . . . . . . . . . CalifVRDL2_2010_01_11
. . . . . . . . . . . . . . . . . . . . . . with syn121P &amp; 502K,
. . . . . . . . . . . . . . . YaroslavlIIV196_2009_12_04_f
. . . . . . . . . . . . . . . . . . . . . . with syn159N, 225G,
. . . . . . . . . . . . . . . WiscD0128_2009_11_15
. . . . . . . . . . . . . . . . . . . . . . with syn343G, 377G, 471H,
. . . . . . . . . . . . . . . Brussels243_2009_11_09
. . . . . . . . . . . . . . . . . . . . . . with syn44L, syn159N &amp; syn323N,
. . . . . . . . . . . . . . . Australia6_2009_07_18
. . . . . . . . . . . . . . . . . . . . . . with syn159N, 233H]
. . . . . . . . syn326S [Wisconsin08_2010_08_10
. . . . . . . . . . . . . . . . . . . . . . with 39R, syn78S, 137T, 186P, syn297N,
. . . . . . . . . . . . . . . . . . . . . . . . . . syn326S, syn346G, syn388K,
. . . . . . . . . . . . . . . . . . . . . . . . . . syn413K, 444K, syn474C,
. . . . . . . . . . . . . . . Zhongyuan1643_2009_11_16
. . . . . . . . . . . . . . . . . . . . . . with 208G],
. . . . . . . . syn383N [H3N8],
. . . . . . . . syn388K [H3N8, H5N1, S7],
. . . . . . . . . . . . . . . [OzVictoria508_2010_07_24
. . . . . . . . . . . . . . . . . . . . . . . with 238D,
. . . . . . . . . . . . . . . swIowa44837_1_2009_11_08_xL
. . . . . . . . . . . . . . . . . . . . . . . with 188R, 225N &amp; 230I,
. . . . . . . . . . . . . . . Utah20_C2_2_2009_07_25_VxX
. . . . . . . . . . . . . . . . . . . . . . . with 159D &amp; 227G, et al],
. . . . . . . . syn411N (AAc) [H7N3 &amp; H7N7 donor ATc],
. . . . . . . . 444K (AAg) [H3N8 gAg],
. . . . . . . . syn474C [H3N8, Avian H1N1 2010],
. . . . . . . . . . . . . . . [Michigan10_2009_06_03 with 137T, 225N, et al])

. . . . IranBandarAbbas5096_2010_10_02 (
. . . . . . . . 72P,
. . . . . . . . 137T (aCA) [H3N8 donor aAT, aGT, aGC],
. . . . . . . . 186P [Avian H12 2008, 2009], [Avian H1N1],
. . . . . . . . syn318A,
. . . . . . . . 377G [H2N3],
. . . . . . . . . . . . . [H5N1 Human Egypt Preschool 2009, H5N1 Human Indonesia 2005],
. . . . . . . . . . . . . [H6N1 2009],
. . . . . . . . . . . . . [US Swine 1957-1978],
. . . . . . . . . . . . . [Florida13_2010_08_02
. . . . . . . . . . . . . . . . . . . with 137T, 186P, syn474C, 512M,
. . . . . . . . . . . . . . Iowa05_2010_05_04
. . . . . . . . . . . . . . . . . . . with 208K, syn516I,
. . . . . . . . . . . . . . swSouthDakota1_2010_04_27,
. . . . . . . . . . . . . . TexasAF2647_2010_04_11
. . . . . . . . . . . . . . . . . . . with 200S, syn214K, syn276H,
. . . . . . . . . . . . . . swIowa02999_2010_04_01,
. . . . . . . . . . . . . . UtahAF2653_2010_03_14
. . . . . . . . . . . . . . . . . . . with syn253V,
. . . . . . . . . . . . . . North Carolina05_2010_02_24
. . . . . . . . . . . . . . . . . . . with 259V,
. . . . . . . . . . . . . . Kentucky04_2010_02_22
. . . . . . . . . . . . . . . . . . . with syn488D,
. . . . . . . . . . . . . . Minnesota02_2010_02_17
. . . . . . . . . . . . . . . . . . . with 259V, 509D,
. . . . . . . . . . . . . . SouthCarolina01_2010_01_27
. . . . . . . . . . . . . . . . . . . with syn238E, syn488D,
. . . . . . . . . . . . . . AnasPlatBelgium04328_pcs17_2010_01_21
. . . . . . . . . . . . . . . . . . . with syn474C,
. . . . . . . . . . . . . . GermanyAF2208_2010_01_20
. . . . . . . . . . . . . . . . . . . with #1T, 225E,
. . . . . . . . . . . . . . NorthCarolina03_2010_01_20
. . . . . . . . . . . . . . . . . . . with 225G mix, 259V, syn275V,
. . . . . . . . . . . . . . NewMexico01_2010_01_05
. . . . . . . . . . . . . . . . . . . with 430I, syn445V, syn490P,
. . . . . . . . . . . . . . swThailandCU_CHK4_2009_01
. . . . . . . . . . . . . . . . . . with #1T, 0A, 189T, syn338G,
. . . . . . . . . . . . . . . . . . . . . . syn451K, syn474C, syn456L, syn529L,
. . . . . . . . . . . . . . Moldova5053_2009_12_11
. . . . . . . . . . . . . . . . . . with 173R, 176I, 225A, 225G mix wt, syn286K,
. . . . . . . . . . . . . . WiscD0128_2009_11_15
. . . . . . . . . . . . . . . . . . . with syn297N, syn343G, 471H,
. . . . . . . . . . . . . . KoreaAF2411_2009_11_11
. . . . . . . . . . . . . . . . . . . with 237I, syn286K,
. . . . . . . . . . . . . . England94840077_2009_11
. . . . . . . . . . . . . . . . . . . with 259T,
. . . . . . . . . . . . . . BahrainN11892_2009_10
. . . . . . . . . . . . . . . . . . . with 208K,
. . . . . . . . . . . . . . Nevada16_2009_10_02
. . . . . . . . . . . . . . . . . . . with 464R mix wt,
. . . . . . . . . . . . . . Tasmania2005_2009_07_03
. . . . . . . . . . . . . . . . . . . with 289M,
. . . . . . . . . . . . . . Texas42303371_2009
. . . . . . . . . . . . . . . . . . . with syn456L, syn507K],
. . . . . . . . syn449Y (TAc) [H3N8 Ttc],
. . . . . . . . syn474C [H3N8])

. . . . IranKaraj5327_2010_12_06 (
. . . . . . . . syn34N [JapanKanagawa74_2010_10_16, et al],
. . . . . . . . 146G [JapanKanagawa74_2010_10_16, et al],
. . . . . . . . 188T,
. . . . . . . . 200T,
. . . . . . . . syn338G,
. . . . . . . . 377K,
. . . . . . . . 454N,
. . . . . . . . syn465N [OzBrisbane209_51F_2010_08_09,
. . . . . . . . . . . . . . . . . . . . with 156E &amp; 225G,
. . . . . . . . . . . . . . CalifVRDL131_2009_12_30, et al],
. . . . . . . . syn538F [Brunei218_2010 with 188T])

<span style="color: #274e13; font-size: x-large;">Australia Sequence</span>

. . . . OzBrisbane209_51F_2010_08_09 (
. . . . . . . . <strong><span style="color: blue;">156E</span></strong> [H2N3, <strong><span style="color: #660000;">H3N8</span></strong>, <strong><span style="color: #660000;">H5N1</span></strong>, H10N7],
. . . . . . . . <strong><span style="color: blue;">188T</span></strong> [H6N1, <strong><span style="color: #660000;">H7N7</span></strong>],
. . . . . . . . <strong><span style="color: blue;">225G</span></strong>,
. . . . . . . . syn338G [<strong><span style="color: #660000;">H3N8</span></strong>, H4, H5, H6, sw],
. . . . . . . . . . . . . . . [OzVictoria670_30M_2010_11_14
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . Emergent across Australia
. . . . . . . . . . . . . . . . . . . . . during late 2010 season,
. . . . . . . . . . . . . . . Florida14_24M_2010_08_05
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . OZVictoria512_2010_07_30
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . NZChristchurch15_2010_07_12
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . India5107_2010_06_28
. . . . . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . . . . . . MississippiAF2474_2010_03_10
. . . . . . . . . . . . . . . . . . . . . with syn235T,
. . . . . . . . . . . . . . . FloridaAF2197_2010_03_07
. . . . . . . . . . . . . . . . . . . . . with 156T,
. . . . . . . . . . . . . . . CalifVRDL9_2010_02_09
. . . . . . . . . . . . . . . . . . . . . with syn193S,
. . . . . . . . . . . . . . . Georgia06_2010_02_05
. . . . . . . . . . . . . . . . . . . . . with syn161Y,
. . . . . . . . . . . . . . . NY4662_2010_02_03
. . . . . . . . . . . . . . . . . . . . . with 97N, syn276H, syn283Q, syn304G,
. . . . . . . . . . . . . . . TexasJMS406_2010_01_10
. . . . . . . . . . . . . . . . . . . . . with 187A, syn193S, syn283Q,
. . . . . . . . . . . . . . . TexasJMS405_2010_01_09
. . . . . . . . . . . . . . . . . . . . . with 187A, syn283Q,
. . . . . . . . . . . . . . . CalifVRDL131_2009_12_30
. . . . . . . . . . . . . . . . . . . . . with 225G, syn455Q,
. . . . . . . . . . . . . . . LouisianaAF2435_2009_11_30
. . . . . . . . . . . . . . . . . . . . . with syn13N,
. . . . . . . . . . . . . . . Vienna291_2009_11_19
. . . . . . . . . . . . . . . catOregon29573_2009_11_09
. . . . . . . . . . . . . . . . . . . . . with 226R, syn283Q,
. . . . . . . . . . . . . . . Calif_SanDiegoINS63_2009_10_26
. . . . . . . . . . . . . . . . . . . . . with syn283Q, et al],
. . . . . . . . 377K,
. . . . . . . . 454N [H7N3, <strong><span style="color: #660000;">H7N7, H9N2</span></strong>]
. . . . . . . . . . [Florida14_24M_2010_08_05
. . . . . . . . . . . . . . . . . . with 188T, 454N,
. . . . . . . . . . FL_Pen210_2009_11_10
. . . . . . . . . . . . . . . . . . with 225E,
. . . . . . . . . . SouthCarolina18_2009_09_16_VxX
. . . . . . . . . . . . . . . . . . with 159D, 224K,
. . . . . . . . . . Texas45131774_2009_09_13
. . . . . . . . . . . . . . . . . . with syn223V,
. . . . . . . . . . IndiaPune9355_2009_08
. . . . . . . . . . . . . . . . . . with 225G,
. . . . . . . . . . IndiaBlore236_2009_06_xL
. . . . . . . . . . . . . . . . . . with 226R, et al],
. . . . . . . . syn465N)


<span style="font-size: x-small;"><strong><span style="color: red;">1.</span></strong> Ellis J, Galiano M, Pebody R, Lackenby A, Thompson C, Bermingham A, McLean E, Zhao H, Bolotin S, Dar O, Watson JM, Zambon M. Virological analysis of fatal influenza cases in the United Kingdom during the early wave of influenza in winter 2010/11. Euro Surveill. 2011;16(1):pii=19760. Available online: </span><a href="http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19760" target="_blank"><span style="font-size: x-small;">http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19760</span></a>

[mixin]

Re: 225G Worldwide Tracking &amp; Evaluation

Disclaimer: I am not a scientist, researcher, programmer or file converter so there could be an occassional error.

I finally have the ability to search submissions from both GISAID and Genbank. Thanks to gs for the hours and hours he spent helping me with this.

To tell the difference between my GISAID isolates and his from Genbank, mine include the method of passage and the collection date is a little different.

This first group contains the mutations that give D225N.

R >A/Castro/12274/2010,2010/05/14,E3/E1
R >A/Moldova/351/2010,2010/01/18,clinical sample
A >A/Christchurch/16/2010,2010/07/12,E2
R >A/Hong Kong/2166/2010,2010/07/13,E1
A >A/Hong Kong/2172/2010,2010/07/12,E3
A >A/Christchurch/16/2010,2010/07/12,E2/E2
A >A/Christchurch/16/2010,2010/07/12,E2/E1
A >A/Kenya/3872/2010,2010/07/13,E3
R >A/Bangladesh/8003/2010,2010/09/16,C2/C1
A >A/Alborz/5607/2010,2010/12/01,E2/E1
A >A/YOKOHAMA/1357/2009,2009/10/06,MDCK 2 +2,
A >A/NIIGATA/1371/2009,2009/11/06,MDCK 2 +1
A >A/YAMAGATA/721/2009,2009/12/08,MDCK 1 +3
A >A/India/4725/2009,2009/06/10,X2/C2
A >A/Neimenggu-Hangjinhouqi/SWL51/2009,2009/11/02,E2
A >A/Fujian-Gulou/SWL11951/2009,2009/11/16,E2
R >A/Ukraine/01/2009,2009/10/28,Original
A >A/Ukraine/02/2009,2009/10/28,Original
R >A/Stockholm/92/2009,2009/08/27,original
R >A/Moldova/G-152/2009,2009/10/23,clinical sample
R >A/Kyiv/377/2009,2009/11/07,lung wash
R >A/Chernihiv/452/2009,2009/11/07,lung wash
R >A/Lviv/425/2009,2009/11/07,lung autopsy
R >A/Lviv/639/2009,2009/11/11,lung autopsy
R >A/Azores/13092/2009,2009/11/12,nasopharyngeal swab
R >A/Rivne/836/2009,2009/11/13,lung autopsy
R >A/Chernivtsi/842/2009,2009/11/11,lung autopsy
R >A/Lviv/420/2009,2009/11/09,lung autopsy
A >A/Lviv/646/2009,2009/11/10,lung autopsy
A >A/Lviv/638/2009,2009/11/08,lung autopsy
R >A/Azores/13091/2009,2009/11/20,bronchial wash
R >A/Ivano-Frankivsk/237/2009,2009/10/26,lung autopsy
R >A/Lviv/421/2009,2009/11/09,lung autopsy
R >A/Lviv/645/2009,2009/11/01,lung autopsy
R >A/Rivne/214/2009,2009/10/30,lung autopsy
A >A/Chernivtsi/844/2009,2009/11/11,lung autopsy
R >A/Cherkasy/744/2009,2009/11/01,lung autopsy
R >A/Chernihiv/859/2009,2009/11/14,lung autopsy
A >A/Baden-Wurttemberg/502/2009,2009/12/06,SIAT3, SIAT1
A >A/Ireland/M79555/2009,2009/11/11,pm lung
A >A/Ireland/M79556/2009,2009/11/11,pm lung
R >A/Arizona/04/2009,2009/04/27,E2
R >A/South Carolina/09/2009,2009/04/26,E2
R >A/Utah/42/2009,2009/07/24,Original
A >A/Michigan/10/2009,2009/06/03,C1-1
A >A/Michigan/10/2009,2009/06/03,C1-2
R >A/North Carolina/42/2009,2009/10/14,IR
A >A/South Carolina/47/2009,2009/11/05,E2
R >A/SYDNEY/2501/2009,2009/07/07,original specimen
A >A/VICTORIA/2125/2009,2009/11/08,Clinical Specimen
A >A/Perth/431/2009,2009/07/28,MDCK2
T >A/Monterrey/3/2009
R >A/Sw/4/Mexico/2009/04/
R >A/South Carolina/09/2009/04/26
R >A/Arizona/04/2009/04/27
A >A/Argentina/HNRG8/2009/06/11
A >A/NY/4099/2009/06/13
A >A/NY/4576/2009/07/08
A >A/Texas/43132503/2009/07/13
R >A/Utah/42/2009/07/24
A >A/Sao Paulo/53838/2009/08/01
A >A/Sao Paulo/53845/2009/08/02
A >A/Malaysia/8860/2009/08/08
R >A/Mexico/InDRE36529/2009/08/08
R >A/Nagano/RC1/2009/08/27
A >A/Nagano/RC1/2009/08/27
R >A/Bahia/15525/2009/09/05
R >A/Mexico/InDRE41741/2009/09/14
R >A/Mexico/InDRE50625/2009/10/31
A >A/Ulyanovsk/CRIE-SHTA/2009/10/31
A >A/Egypt/N14648/2009/11/
R >A/Mexico/InDRE50617/2009/11/01
A >A/Russia/100/2009/11/01
A >A/Netherlands/2457b/2009/11/07
A >A/Moscow oblast/CRIE-STV/2009/11/09
A >A/Orenburg/IIV2974/2009/11/16
R >A/Orel/CRIE-AAP/2009/11/20
R >A/Moscow/CRIE-TYD/2009/11/23
A >A/Tambov/CRIE-SPN/2009/11/24
R >A/Ivanovo/CRIE-RNA/2009/11/24
A >A/Ankara/26/2009/11/24
R >A/Tambov/CRIE-LLA/2009/11/30
A >A/Netherlands/2629/2009/12/04
A >A/Yaroslavl/IIV-198/2009/12/05
715  haall 84   -:132 A:42 G:9143 R:41 T:1

This group gives mutations for D225G

G >A/Zhejiang-Haishu/SWL1289/2010,2010/02/11,E1
G >A/Arizona/04/2010,2010/04/03,C1
G >A/Florida/04/2010,2010/03/18,E2
G >A/Florida/04/2010,2010/03/18,CX/C1
G >A/Saratov/07/2010,2010/02/01,E2
G >A/Saratov/07/2010,2010/02/01,E2/E1
G >A/St. Petersburg/204/2010,2010/02/08,E2
G >A/St. Petersburg/204/2010,2010/02/08,E2/E1
G >A/Wisconsin/04/2010,2010/02/22,E3
G >A/Kentucky/06/2010,2010/04/06,MX/C1
G >A/Brazil/8546/2010,2010/03/04,C1
R >A/Moldova/351/2010,2010/01/18,clinical sample
G >A/Belgorod/2/2010,2010/03/15,E1, E1
G >A/Victoria/502/2010,2010/06/20,E3
R >A/Montana/01/2010,2010/04/04,E2
G >A/Brazil/1394/2010,2010/03/25,C1
G >A/St. Petersburg/204/2010,2010/02/08,E2/E2
G >A/Hong Kong/2200/2010,2010/07/14,MDCK2, MDCK1
G >A/Pennsylvania/07/2010,2010/08/12,C1
G >A/SYDNEY/202/2010,2010/07/18,mdckx,mdck1
G >A/BRISBANE/209/2010,2010/08/09,MDCK4
G >A/Venezuela/27/2010,2010/01/29,C1
G >A/England/4880378/2010,2010/12/01,original
G >A/England/4940476/2010,2010/12/01,original
G >A/Kentucky/09/2010,2010/11/01,E2
G >A/Saudi Arabia/4270/2009,2009/07/28,C1
G >A/Tianjin/SWL4/2009,2009/06/11,E3
G >A/NAGANO/RC1/2009,2009/08/27,MDCK1
G >A/India/8489/2009,2009/10/20,X3/C1
R >A/KathmanduBGN/21-2/2009,2009/11/30,nasal swab
R >A/YAMAGUCHI/247/2009,2009/12/01,MDCK 2
G >A/Egypt-Vacsera/138/2009,2009/12/01,E1,E1
R >A/Laos/138/2009,2009/08/22,C2
G >A/Iran/572/2009,2009/12/01,Ex, E1
R >A/Iran/20001/2009,2009/12/01,Ex, E1
G >A/Georgia/4484/2009,2009/12/21,clinical specimen
G >A/Turkey/TR-20/2009,2009/10/31,SIAT1, SIAT3
R >A/Shanxi-Linxian/SWL323/2009,2009/11/04,C1
G >A/Guangdong-Luohu/SWL1755/2009,2009/11/05,E2
G >A/Zhejiang/SWL3/2009,2009/09/06,C2
G >A/Georgia/4484/2009,2009/12/21,SIAT4
R >A/TOKYO/99946/2009,2009/11/05,E1 +1
G >A/Lviv/N2/2009,2009/10/28,lungs
G >A/Ternopil/N10/2009,2009/10/28,lungs
G >A/Ternopil/N11/2009,2009/10/27,lungs
G >A/Lviv/N6/2009,2009/10/27,throat swab
G >A/Stockholm/48/2009,2009/07/15,original
R >A/Stockholm/92/2009,2009/08/27,original
R >A/Dnipropetrovsk/273/2009,2009/11/05,MDCK1
R >A/Moldova/G-152/2009,2009/10/23,clinical sample
V >A/Kyiv/377/2009,2009/11/07,lung wash
R >A/Chernihiv/452/2009,2009/11/07,lung wash
G >A/Ivano-Frankivsk/240/2009,2009/10/29,lung autopsy
R >A/Lviv/425/2009,2009/11/07,lung autopsy
G >A/Kyiv/251/2009,2009/11/04,lung autopsy
G >A/Ivano-Frankivsk/239/2009,2009/10/28,lung autopsy
G >A/Rivne/516/2009,2009/11/10,lung autopsy 
R >A/Lviv/639/2009,2009/11/11,lung autopsy
R >A/Zakarpatska/830/2009,2009/11/12,lung autopsy
R >A/Rivne/836/2009,2009/11/13,lung autopsy
R >A/Chernivtsi/842/2009,2009/11/11,lung autopsy
R >A/Lviv/420/2009,2009/11/09,lung autopsy
R >A/Azores/13091/2009,2009/11/20,bronchial wash
R >A/Ivano-Frankivsk/237/2009,2009/10/26,lung autopsy
R >A/Lviv/421/2009,2009/11/09,lung autopsy
R >A/Lviv/645/2009,2009/11/01,lung autopsy
R >A/Rivne/214/2009,2009/10/30,lung autopsy
R >A/Sumy/797/2009,2009/11/13,lung autopsy
R >A/Cherkasy/744/2009,2009/11/01,lung autopsy
G >A/Volin/226/2009,2009/11/05,Siat3
G >A/Rivne/214/2009,2009/10/30,Siat4
R >A/Chernihiv/859/2009,2009/11/14,lung autopsy
R >A/Chernihiv/856/2009,2009/11/13,lung autopsy
R >A/Chernihiv/855/2009,2009/11/11,lung autopsy
R >A/Chernihiv/857/2009,2009/11/14,lung autopsy
G >A/Voronezh/01/2009,2009/11/30,E1/E1
R >A/France/6808/09,2009/11/01,C1, SIAT2
G >A/Latvia/12-1488/2009,2009/12/29,MDCKx
G >A/Latvia/12-2498/2009,2009/12/07,MDCKx
G >A/Iceland/162/2009,2009/10/21,MDCK2
G >A/Iceland/176/2009,2009/11/01,MDCK2
G >A/Denmark/1900/2009,2009/12/01,MDCK3, SIAT1
G >A/Iceland/162/2009,2009/10/21,MDCK2, SIAT3
G >A/Latvia/12-1488/2009,2009/12/29,MDCKx, SIAT1
G >A/Moldova/3163/2009,2009/11/23,clinical sample
G >A/Moldova/3245/2009,2009/11/25,clinical sample
V >A/Moldova/5053/2009,2009/12/11,clinical sample
G >A/ENG/671/2009,2009/07/01,c1
G >A/ENG/94640080/2009,2009/11/04,original
G >A/ENG/93960032/2009,2009/09/10,original
G >A/SC/10/2009,2009/06/01,original
G >A/ENG/93780689/2009,2009/09/01,c1
G >A/ENG/1005/2009,2009/09/01,c1
R >A/ENG/94620048/2009,2009/10/01,original
R >A/California/07/2009,2009/04/09,E2
G >A/Mexico/3955/2009,2009/04/03,E2
G >A/New York/04/2009,2009/04/01,E2
R >A/New York/11/2009,2009/04/25,E2
R >A/New York/31/2009,2009/04/24,E2
G >A/Georgia/01/2009,2009/04/27,C1
R >A/Nebraska/02/2009,2009/04/01,E2
R >A/Texas/04/2009,2009/04/14,X/E2
G >A/Texas/11/2009,2009/04/23,E2
R >A/Texas/10/2009,2009/04/23,E2
R >A/California/13/2009,2009/04/21,E2
G >A/Texas/05/2009,2009/04/15,X/E2
G >A/Illinois/10/2009,2009/07/31,C4
G >A/Indiana/09/2009,2009/04/22,E2
R >A/Massachusetts/16/2009,2009/06/15,C1
G >A/Utah/42/2009,2009/07/24,M1
R >A/Utah/42/2009,2009/07/24,Original
G >A/Costa Rica/3149/2009,2009/07/13,X1/C1
G >A/Michigan/25/2009,2009/10/14,C1
G >A/Utah/42/2009,2009/07/24,M1/C1
G >A/Mexico/3956/2009,2009/04/03,Original
R >A/North Carolina/39/2009,2009/10/15,IR
G >A/North Carolina/49/2009,2009/10/16,M1/C1
G >A/North Carolina/53/2009,2009/10/18,M1/C1
G >A/Washington/68/2009,2009/10/14,X/C2
G >A/Jamaica/6261/2009,2009/07/07,C1
G >A/Uruguay/755/2009,2009/07/22,C1
G >A/SYDNEY/2503/2009,2009/07/12,original specimen
R >A/SOUTH AUSTRALIA/2016/2009,2009/07/16,MDCKX,MDCK1
G >A/Perth/261/2009,2009/08/12,MDCKX,MDCK1
T >A/Monterrey/3/2009
G >A/Mexico/InDRE4114/2009
G >A/Mexico/InDRE4114/2009
G >A/Vladivostok/01/2009
G >A/Abakan/02/2009
G >A/Tomsk/07/2009
G >A/Salekhard/01/2009
G >A/Tomsk/08/2009
R >A/Sw/4/Mexico/2009/04/
R >A/Nebraska/02/2009/04/
G >A/NY/04/2009/04/
G >A/Mexico/3955/2009/04/02
R >A/CA/07/2009/04/09
R >A/Texas/04/2009/04/14
G >A/Texas/05/2009/04/15
R >A/CA/13/2009/04/21
R >A/Texas/10/2009/04/23
G >A/Texas/11/2009/04/23
R >A/NY/31/2009/04/24
R >A/NY/11/2009/04/25
G >A/Georgia/01/2009/04/27
G >A/Mexico city/CIA11/2009/05/02
T >A/Sw/Alberta/OTH-33-3/2009/05/03
G >A/Mexico city/CIA10/2009/05/03
R >A/Boston/109/2009/06/15
G >A/Hiroshima/201/2009/06/17
G >A/CA/VRDL7/2009/06/17
M >A/CA/VRDL31/2009/06/17
G >A/CA/VRDL27/2009/06/27
G >A/Argentina/HNRG16/2009/06/29
R >A/Texas/42291877/2009/06/29
G >A/Sao Paulo/53206/2009/07/19
G >A/Rio de Janeiro/4707/2009/07/23
R >A/Finland/614/2009/07/24
R >A/Utah/42/2009/07/24
G >A/Utah/42/2009/07/24
G >A/Rio de Janeiro/5826/2009/07/28
G >A/Catalonia/NS1706/2009/07/29
N >A/Singapore/GP2312/2009/07/31
G >A/Pune/NIV9355/2009/08/
G >A/Mum/NIV9312/2009/08/
G >A/Rio de Janeiro/7131/2009/08/01
G >A/Sao Paulo/53225/2009/08/01
G >A/Catalonia/NS2001/2009/08/03
G >A/Catalonia/NS2008/2009/08/03
G >A/Rio de Janeiro/7455/2009/08/04
N >A/Singapore/GP2641/2009/08/05
N >A/Singapore/GP2651/2009/08/05
R >A/Norway/2924/2009/08/10
G >A/Orenburg/CRIE-VNV/2009/08/16
G >A/Rio de Janeiro/14601/2009/08/24
G >A/Roma/ISS1897/2009/08/27
G >A/Roma/ISS1941/2009/08/27
R >A/Nagano/RC1/2009/08/27
G >A/Pune/NIV10278/2009/09/
G >A/Netherlands/1064b/2009/09/01
G >A/Norway/3206-3/2009/09/01
R >A/Bahia/15525/2009/09/05
G >A/South Carolina/WRSP520/2009/09/05
G >A/Zhejiang-Yiwu/11/2009/09/06
G >A/Zhejiang/DTID-ZJU02/2009/09/07
G >A/Zhejiang/DTID-ZJU03/2009/09/07
G >A/Nsk/NIV10348/2009/09/07
R >A/Mexico/InDRE43149/2009/09/28
R >A/Moscow/CRIE-BVA/2009/10/24
R >A/Mexico/InDRE50625/2009/10/31
R >A/Kaluga/CRIE-INV/2009/11/
R >A/Ivanovo/CRIE-AMV/2009/11/
G >A/Moscow oblast/CRIE-DEI/2009/11/
G >A/Egypt/N14644/2009/11/01
R >A/Mexico/InDRE50617/2009/11/01
G >A/Moscow oblast/CRIE-OAM/2009/11/02
R >A/Lipetsk/CRIE-BVV/2009/11/10
R >A/Yaroslavl/CRIE-CHMV/2009/11/10
G >A/Bryansk/IIV2971/2009/11/11
G >A/Tver/IIV2969/2009/11/14
G >A/Thessaloniki/2502/2009/11/17
G >A/Netherlands/2243/2009/11/17
C >A/Madrid/INS296/2009/11/17
G >A/Kastoria/2636/2009/11/18
G >A/Ivanovo/CRIE-BOV/2009/11/18
G >A/Ivanovo/CRIE-AIV/2009/11/18
R >A/Orel/CRIE-AAP/2009/11/20
G >A/Ivanovo/CRIE-STV1/2009/11/21
G >A/Thessaloniki/2812/2009/11/22
G >A/Moscow/CRIE-CHSN/2009/11/23
R >A/Moscow oblast/CRIE-KMA/2009/11/23
G >A/Ulyanovsk/CRIE-BAA/2009/11/23
R >A/Moscow/CRIE-TYD/2009/11/23
R >A/Ivanovo/CRIE-RNA/2009/11/24
G >A/Perm/01/2009/11/25
G >A/Tver/IIV-183/2009/11/25
R >A/Tambov/CRIE-LLA/2009/11/30
G >A/Taraz/01/2009/12/
G >A/Yaroslavl/IIV-196/2009/12/04
G >A/IIV-Anadyr/177/2009/12/04
G >A/Catalonia/NS092145684/2009/12/10
R >A/NY/7216/2009/12/21
G >A/Iran/1519/2009/12/23
G >A/CA/VRDL131/2009/12/30
G >A/Egypt/N00267/2010/01/
G >A/Karasuk/01/2010/01/04
G >A/Kavala/219/2010/01/12
G >A/Thessaloniki/206/2010/01/12
G >A/Thessaloniki/225/2010/01/13
G >A/Tunisia/1064/2010/01/18
G >A/Orenburg/IIV-13/2010/03/02
G >A/Orenburg/IIV-13/2010/03/02
R >A/Singapore/SS003/2010/04/
R >A/Singapore/SS004/2010/04/
R >A/Bahia/231/2010/04/01
G >A/Nanjing/2/2010/06/30
G >A/Nanjing/1/2010/06/30
716  haall 244   -:132 A:8983 C:1 G:159 M:1 N:3 R:76 T:2 V:2