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  • rglfgaiagfieggw

    rglfgaiagfieggw

    GLFGAIAGFIEGGW

    this is a strangely conserved epitope in influenza in most of the
    17 HA-subtypes and even in influenza B. (where it is GFFGAIAGFLEGGW

    what's it's function, why is it conserved, can we target
    it in universal vaccine

    I couldn't find something googling this keyword

    there is no other such epitope


    position 346-359 (362-375 in other numbering) - , 363-376 in flu-B


    it's directly following the cleavage-site, so at the beginning of HA2
    I'm interested in expert panflu damage estimates
    my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

  • #2
    Re: rglfgaiagfieggw

    seems that I should have searched for GLFGAIAGFIEGGW instead
    the first R belonged to the cleavage site and then comes the
    "fusion peptide"


    Lorieau et al. 10.1073/pnas.1213801109
    SI Materials and Methods.
    NMR Sample Preparation. Serotype H1 HAfp23 and HAfp23-
    G8A fusion peptides, with the sequence GLFGAIA[G/A]FI
    EGGWTGMIDG WYGSGKKKKD, and the HAfp14 peptide,
    with the sequence of GLFGAIAGFIEGGWKKKKD, were ex-
    pressed with a high-solubility tag (1) (underlinedsequence) and
    purified as previously described (2)

    2. Lorieau JL, Louis JM, Bax A (2010) The complete influenza hemagglutinin fusion
    domain adopts a tight helical hairpin arrangement at the lipid:water interface. Proc
    Natl Acad Sci USA 107(25):11341–11346.

    ---------------------------------------------------------------
    http://en.wikipedia.org/wiki/Hemagglutinin_(influenza)
    This so-called "fusion peptide" acts like a molecular grappling hook by inserting itself into the
    endosomal membrane and locking on. Then, when the rest of the HA molecule refolds into a
    new structure (which is more stable at the lower pH), it "retracts the grappling hook" and pulls
    the endosomal membrane right up next to the virus particle's own membrane, causing the two
    to fuse together. Once this has happened, the contents of the virus, including its RNA genome,
    are free to pour out into the cell's cytoplasm.
    ------------------------------------------------------------------
    http://www.ncbi.nlm.nih.gov/pubmed/21078301
    As the fusion peptide is the only universally conserved epitope in all influenza A and B viruses,
    it could be an attractive target for vaccine-induced immune responses.
    -----------------------------------------------------

    http://www.ncbi.nlm.nih.gov/pubmed/21078301
    As the fusion peptide is the only universally conserved epitope in all influenza A and B viruses,
    it could be an attractive target for vaccine-induced immune responses.

    http://www.ncbi.nlm.nih.gov/pubmed/23144542
    ...Our strategy to design AVPs seems to be very promising since the peptides were
    synthetized and their antiviral activities have produced very encouraging results.
    I'm interested in expert panflu damage estimates
    my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

    Comment


    • #3
      Re: rglfgaiagfieggw

      in avian influenza-A almost (>99&#37 all fusion peptides are
      GLFGAIAGFIEGGW or GLFGAIAGFIENGW


      Code:
      count,sequence
      --------------------
         9085,GLFGAIAGFIEGGW
         3269,GLFGAIAGFIENGW
           44,GIFGAIAGFIEGGW
           30,GIFGAIAGFIENGW
           15,GLFGAKAGFIEGGW
           21,GLFGAKAGFIENGW

      if this is just a "key" for cell-entry, then I'd just change the lock,
      create genetically manipulated chickens (...--> humans)

      however, what is the lock made for, what useful things use it to
      get into the cell ? We would have to tell these things when and how the lock was changed

      --------------------------------------------------

      http://repository.tamu.edu/bitstream...pdf?sequence=2
      The N-terminal segment of HA2 subunit, composed of twenty nonpolar amino acids
      (1GLFGAIAGFIENGWEGMIDG20), is required for endosomal fusion (56,63,64).

      56. Han, X., Bushweller, J. H., Cafiso, D. S., and Tamm, L. K. (2001) Nat Struct
      Biol 8, 715-720
      63. Esbjorner, E. K., Oglecka, K., Lincoln, P., Graslund, A., and Norden, B. (2007)
      Biochemistry 46, 13490-13504
      64. Wharton, S. A., Martin, S. R., Ruigrok, R. W., Skehel, J. J., and Wiley, D. C.
      (1988) J Gen Virol 69 ( Pt 8), 1847-1857


      GLFGAIAGFIENGWEGMIDG

      -------------------------------------

      I couldn't find GLFGAIAG or reversed in the human genome
      so, what is intended to be let in by that key in normal cell life ?

      -------------------------------------------------------------

      ..... PENPKTR/GLF
      A/Ck/BC/2004 : PENPKQAYQKRMTR/GLF
      A/Ck/Chile/2002 PENPKTCSPLSRCRETR/GLF
      A/Ty/Oregon/71 : PENPKTSLSPLYPGRTTDLQVPTAR/GLF
      A/Seal/Mass/1/80 : PENPKKEHPSAGKDPKKTGGPIYRRTR/GLF
      I'm interested in expert panflu damage estimates
      my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

      Comment


      • #4
        Re: glfgaiagfieggw

        glfgaiagfieggw could be the reason why flu strains die out,
        why a H3-infection protects from a H1-infection for some months
        http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3163940/

        (speculative)
        -------------------------------------------------------------
        Fully human broadly neutralizing monoclonal antibodies against influenza A viruses
        generated from the memory B cells of a 2009 pandemic H1N1 influenza vaccine recipient.
        FIEGGWTGMVDGWYGYHH in pH1N1 vaccinees
        --------------------------------------------------------------
        Two distinct regions of HA2 glycopolypeptide of influenza virus hemagglutinin elicit
        cross-protective immunity against influenza.
        most efficient protection was achieved with EHA2 against homologous
        ---------------------------------------------------------------------
        A human CD4+ T cell epitope in the influenza hemagglutinin is cross-reactive to influenza
        A virus subtypes and to influenza B virus.
        Almost all donors who responded to the epitope had the HLA-DRB1*09 allele, Although natural
        infection or standard vaccination may not induce strong T and B cell responses to the fusion peptide,
        it may be possible to develop a vaccination strategy to induce these CD4(+) T cells,
        ---------------------------------------------------------------------------
        Bovine lactoferrin-derived peptides as novel broad-spectrum inhibitors of influenza virus.
        bLf C-lobe strongly binds to the fusion peptide
        --------------------------------------------------------------------
        Quantitative analyses of all influenza type A viral hemagglutinins and neuraminidases
        using universal antibodies in simple slot blot assays.
        The antibody against the Uni-1 epitope of HA was able to bind to 13 subtypes HA (H1-H13)
        ---------------------------------------------------------------------------------------------
        Heterosubtypic protective immunity against influenza A virus induced by fusion peptide
        of the hemagglutinin in comparison to ectodomain of M2 protein.
        The lower, but still effective protection induced by the fusion peptide
        --------------------------------------------------------------------------------
        http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648274/
        Monoclonal antibodies against the fusion peptide of hemagglutinin protect mice from
        lethal influenza A virus H5N1 infection.
        ---------------------------------------------------------------------------------------------
        A/seal/Mass/1/80 (H7N7) mutants were obtained by activation by thermolysin
        protective immunity in chickens after an asymptomatic infection.
        insertion of leucine at position 4 into the fusion peptide
        -----------------------------------------------------------------------------------------
        I'm interested in expert panflu damage estimates
        my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

        Comment


        • #5
          update, 2015/11/21

          37945,GLFGAIAGFIEGGW
          7758,GLFGAIAGFIENGW
          353,GLFGAIAG}/----
          300,GLFGAIAGFIEGG}
          190,GLFGAIA}/-----
          145,GLFGAIAGFLENGW
          101,GLFGAIAGFI}/--
          77,GLFGAIAGF}/---
          47,GLFGAIAGFIE}/-
          42,GLFGAIAGFIEG}/
          28,GLFGAIAGFLEGGW
          14,GLFGAIAGFIEXGW
          14,GLFGAIAGFIERGW
          10,GLFGAIAGFIENG}
          5,GLFGAIAGFIEN}/
          5,GLFGAIAGFIEGGX
          5,GLFGAIAGFIEGGG
          4,GLFGAIAGSIEGGW
          4,GLFGAIAGFMEGGW
          4,GLFGAIAGFIXGGW
          4,GLFGAIAGFIKGGW
          4,GLFGAIAGFIEGRW
          3,GLFGAIARFIEGRW
          3,GLFGAIAKFYDNDK
          3,GLFGAIAGXIEGGW
          3,GLFGAIAGFVEGGW
          3,GLFGAIAGFTEGGW
          3,GLFGAIAGFMEGGG
          3,GLFGAIAGFIEGVW
          3,GLFGAIAGFIEGGR
          3,GLFGAIAGFIEEGW
          2,GLFGAIAXFIEGGW
          2,GLFGAIARFIEGGW
          2,GLFGAIAKSYINDK
          2,GLFGAIAGLIEGGW
          2,GLFGAIAGFLEGDW
          2,GLFGAIAGFIZGGW
          2,GLFGAIAGFIGGGW
          2,GLFGAIAGFIESGW
          2,GLFGAIAGFIEGGC
          2,GLFGAIAGFIEAGW
          2,GLFGAIAGFIDRGW
          1,GLFGAIAVFIVGGW
          1,GLFGAIASFIKGGW
          1,GLFGAIASFIEGGW
          1,GLFGAIARFIENGW
          1,GLFGAIAKSYTYDK
          1,GLFGAIAKSYDNDK
          1,GLFGAIAKFYVNDK
          1,GLFGAIAIFIEGAW
          1,GLFGAIAGYREGGW
          1,GLFGAIAGYIEGGW
          1,GLFGAIAGSIENGW
          1,GLFGAIAGL}/---
          1,GLFGAIAGLIEGVV
          1,GLFGAIAGIIEGGW
          1,GLFGAIAGFXENGW
          1,GLFGAIAGFXEGGW
          1,GLFGAIAGFVENGW
          1,GLFGAIAGFTENGW
          1,GLFGAIAGFM}/--
          1,GLFGAIAGFMRGDD
          1,GLFGAIAGFMKNGW
          1,GLFGAIAGFMEGDW
          1,GLFGAIAGFMAGRW
          1,GLFGAIAGFLRKGA
          1,GLFGAIAGFLQRGW
          1,GLFGAIAGFLEGGR
          1,GLFGAIAGFIVGGW
          1,GLFGAIAGFIQGGW
          1,GLFGAIAGFIG}/-
          1,GLFGAIAGFIEYGW
          1,GLFGAIAGFIERGV
          1,GLFGAIAGFIERGG
          1,GLFGAIAGFIEPGW
          1,GLFGAIAGFIENDG
          1,GLFGAIAGFIEKDG
          1,GLFGAIAGFIEGSW
          1,GLFGAIAGFIEGNR
          1,GLFGAIAGFIEGGM
          1,GLFGAIAGFIEGGE
          1,GLFGAIAGFIEGEW
          1,GLFGAIAGFIEGA}
          1,GLFGAIAGFIDRSW
          1,GLFGAIAGFFENGW
          1,GLFGAIAGFFEGGW
          1,GLFGAIAGCRPEK}
          1,GLFGAIAGCIEGGW
          1,GLFGAIADFIEGGW
          1,GLFGAIACFIEGGW
          1,GLFGAIAAFIEGGW


          I'm interested in expert panflu damage estimates
          my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

          Comment

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