new flu gene found

[gsgs]

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http://blogs.discovermagazine.com/notrocketscience/2012/06/28/new-flu-gene-found-hiding-in-plain-sight-and-affects-severity-of-infections/

called PA-X , reading frame +1 in segment 3 , codons 190-253

most common avian PA-X es are :

PFVSPKEAKRQLKKDLKSQEPCAGLPTKVSHRTSPALKTLEPMWMDSNRT AALRASFLKCQKK,609
PFVSPREAKRQLKKDLKLQEPCAGSPTKVSHRTSPALKTLEPMWMDSNRT AALRASFLKCPKK,497
PFVSPREAKRQLKKDLKSQEPCAGLPTKVSHRTSPALKTLEPMWMDSNRT AALRASFLKCQKK,443
PFVSPKEAKRQLKKDLKSQEPCVGLPTKVSHRTSPALKTLEPMWMDSNRT AALRASFLKCQKK,307
PFVNPREAKRQLKKNLKSLEPCADLPTKVSHRTSPALKTLEPMWMDSNRT AALRASFLKCQKK,283
PFVSPREAKRQLKKDLKLQEPCAGLPTKVSHRTSPALKTLEPMWMDSNRT AALRASFLKCQKK,182
PFVNPREAKRQLKKDLKSQEPCAGLPTKVSHRTSPALKILEPMWMDSNRT AALRASFLKCQKK,125

bird index
>A/Dk/Altai/1285/1991,1991/08/15,H5N3,Russia
PFVSPREAKRQLKKDLKSQEPCAGLPTKVSHRTSPALKTLEPMWMDSNRT AALRASFLKCQKR

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[tetano]

Re: new flu gene found

See the original article here

[gsgs]

Re: new flu gene found

this could give another guideline how to easily manipulate an existing
successful virus so it becomes more virulent.
Indeed, they had done this with the 1918 virus in mice.

Should such "tricks" how to increase virulence of viruses be published ?
Ask NSABB. Apparently this wasn't done, else we'd heard about it.

[gsgs]

Re: new flu gene found

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3498833/

Ribosomal frameshifting used in influenza A virus expression occurs within the
sequence UCC_UUU_CGU and is in the +1 direction

TCCTTTCGT
In analyses of codon usage in PA, it was observed that the motif TCC_TTT_CGT is extremely
highly conserved at the 5′ end

mutating the UCC codon to AGC (serine) or to GGG, CCC or AAA resulted in a 40 to 70 per cent
reduction in the frameshifting efficiency [30].

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TCCTTTCGT found in genbank release 194.0 : (flu-A only)
segment 1: 0/22089
segment 2:160/22018
segment 3:19599/21860
segment 4:0/64697
segment 5:4/22275
segment 6:1/37514
segment 7:35/28818
segment 8:0/23376

[gsgs]

Re: new flu gene found

14th influenza A polypeptide:
(segment 7)

http://www.ncbi.nlm.nih.gov/pubmed/23133386