Announcement

Collapse
No announcement yet.

Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Modifie

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Modifie

    Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Modified Live-Virus Vaccine

    Journal of Virology, November 2006, p. 11009-11018, Vol. 80, No. 22
    0022-538X/06/$08.00+0 doi:10.1128/JVI.00787-06


    J?rgen A. Richt,1,* Porntippa Lekcharoensuk,2, Kelly M. Lager,1 Amy L. Vincent,1 Christina M. Loiacono,2 Bruce H. Janke,2 Wai-Hong Wu,2 Kyoung-Jin Yoon,2 Richard J. Webby,3 Alicia Sol?rzano,4 and Adolfo Garc?a-Sastre4

    http://jvi.asm.org/cgi/content/abstract/80/22/11009

    Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010,1 Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011,2 Division of Virology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105,3 Department of Microbiology, Mount Sinai School of Medicine, New York, New York 100294

    Received 18 April 2006/ Accepted 22 August 2006

    Swine influenza viruses (SIV) naturally infect pigs and can be transmitted to humans. In the pig, genetic reassortment to create novel influenza subtypes by mixing avian, human, and swine influenza viruses is possible. An SIV vaccine inducing cross-protective immunity between different subtypes and strains circulating in pigs is highly desirable. Previously, we have shown that an H3N2 SIV (A/swine/Texas/4199-2/98 [TX98]) containing a deleted NS1 gene expressing a truncated NS1 protein of 126 amino acids, NS1{blacktriangleup}126, was attenuated in swine. In this study, 4-week-old pigs were vaccinated with the TX98 NS1{blacktriangleup}126 modified live virus (MLV). Ten days after boosting, pigs were challenged with wild-type homologous H3N2 or heterosubtypic H1N1 SIV and sacrificed 5 days later. The MLV was highly attenuated and completely protected against challenge with the homologous virus. Vaccinated pigs challenged with the heterosubtypic H1N1 virus demonstrated macroscopic lung lesions similar to those of the unvaccinated H1N1 control pigs. Remarkably, vaccinated pigs challenged with the H1N1 SIV had significantly lower microscopic lung lesions and less virus shedding from the respiratory tract than did unvaccinated, H1N1-challenged pigs. All vaccinated pigs developed significant levels of hemagglutination inhibition and enzyme-linked immunosorbent assay titers in serum and mucosal immunoglobulin A antibodies against H3N2 SIV antigens. Vaccinated pigs were seronegative for NS1, indicating the potential use of the TX98 NS1 126 MLV as a vaccine to differentiate infected from vaccinated animals.

  • #2
    Re: Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Mod

    full paper available now:

    in html:
    http://jvi.asm.org/cgi/content/full/80/22/11009

    in .pdf:
    http://jvi.asm.org/cgi/reprint/80/22/11009
    I'm interested in expert panflu damage estimates
    my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgT

    Comment


    • #3
      Re: Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Mod

      Here are the details on targets for commercial product sold by Schering Plough (although they don't give the exact composition of their vaccine)

      RF: In the US, classical H1N1, the new reassortant H1N1, three clusters of H3N2 and H1N2 have been reported in US herds. The H3N2 clusters are Cluster I, which contains the Texas-98 virus, Cluster II, which contains a Colorado-99 virus and Cluster III, which contains an Illinois-99 virus. The H3N2s most often isolated have been from Clusters I and III.



      http://www.xl3.info/pdf/interview3.htm

      Comment


      • #4
        Re: Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Mod

        Here is a paper from 2001, looking at cross reactivity of older vaccines. It indicates that H1N1 is represented by a NJ isolate from 1976 or the Netherlands from 1980

        Swine influenza vaccines were first licensed in Europe
        in the mid-1980s. These vaccines are inactivated whole
        virus or split virus preparations for intramuscular administration.
        They contain one representative of the
        H1N1 and H3N2 subtypes in combination with an oil
        adjuvant. Most vaccines include A
        /Port Chalmers/1/73

        (H3N2) and either the human A
        /New Jersey/8/76
        (H1N1) or the Sw
        /Netherlands/25/80 (H1N1) strain.

        No mention of 1977 from Tennesee.

        Vaccine 19 (2001) 4479?4486
        Efficacy of vaccination of pigs with different H1N1 swine
        influenza viruses using a recent challenge strain and different
        parameters of protection
        Kristien Van Reeth *, Geoffrey Labarque, Sophie De Clercq, Maurice Pensaert
        Laboratory of Virology
        , Faculty of Veterinary Medicine, Ghent Uniersity, Salisburylaan 133, B-9820, Merelbeke, Belgium

        Received 28 January 2001; received in revised form 17 May 2001; accepted 21 May 2001

        Comment


        • #5
          Re: Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Mod

          Originally posted by niman View Post
          Here are the details on targets for commercial product sold by Schering Plough (although they don't give the exact composition of their vaccine)

          RF: In the US, classical H1N1, the new reassortant H1N1, three clusters of H3N2 and H1N2 have been reported in US herds. The H3N2 clusters are Cluster I, which contains the Texas-98 virus, Cluster II, which contains a Colorado-99 virus and Cluster III, which contains an Illinois-99 virus. The H3N2s most often isolated have been from Clusters I and III.



          http://www.xl3.info/pdf/interview3.htm
          This paper suggests that the Schering Plough vaccine targets are similar or identical to the late 1990 isolates described above

          The sequences of the
          NC isolates were compared to that previously determined for
          the vaccine strain and to the sequences published previously
          for A/Swine/TX/4199-2/98 (TX98), A/Swine/NC/35922/98
          (NC98); A/Swine/CO/23619/99 (CO99) and A/Swine/OK/
          18089/99 (OK99) (4,7,9).

          http://www.unipr.it/arpa/facvet/dip/...03/267-268.pdf

          Comment


          • #6
            Re: Vaccination of Pigs against Swine Influenza Viruses by Using an NS1-Truncated Mod

            Originally posted by niman View Post
            Here are the details on targets for commercial product sold by Schering Plough (although they don't give the exact composition of their vaccine)

            RF: In the US, classical H1N1, the new reassortant H1N1, three clusters of H3N2 and H1N2 have been reported in US herds. The H3N2 clusters are Cluster I, which contains the Texas-98 virus, Cluster II, which contains a Colorado-99 virus and Cluster III, which contains an Illinois-99 virus. The H3N2s most often isolated have been from Clusters I and III.



            http://www.xl3.info/pdf/interview3.htm
            It seems that the interview was removed not long after you posted that, or their server has been down for a few days. All that is left active is this:

            http://www.xl3.info/

            The Pigsite still has some information captured about the vaccine here, but all the links on the right sidebar to the manufacturer are broken.

            Here is some info about the vaccine on the Pigsite:

            "# Features a new variant or ?reassortant? strain of the H1N1 subtype in addition to the classical H1N1 subtype that has been circulating in U.S. hogs for decades.

            # Contains an H3N2 subtype that protects against the Cluster I Texas-like H3N2 subtype, as well as cross protects against the Cluster III variants of H3N2. "


            There are some archived field reports that show how tough the rH1N1 is
            here and here.

            Then here is a roundtable discussion about finding vaccines for what they say has become a 'destablized' swine flu virus since 1998.
            "he basis for the so-called destabilization of swine influenza seems to be
            unique to North America since 1998. With the emergence of the
            reassortant H3N2, it became clear that that virus has very broad ability to
            reassort with other strains of virus co-circulating in herds at the same time
            and, thereby, creating a new virus that may have a different antigenic
            structure; for example, the reassortant H1N1 that?s so variable because of
            many factors and driven by internal genetic components of the virus."
            _____________________________________________

            Ask Congress to Investigate COVID Origins and Government Response to Pandemic H.R. 834

            i love myself. the quietest. simplest. most powerful. revolution ever. ---- nayyirah waheed

            (My posts are not intended as advice or professional assessments of any kind.)
            Never forget Excalibur.

            Comment

            Working...
            X