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Characterization of influenza A virus with nine segments: Effect gene segment on virus property

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  • Characterization of influenza A virus with nine segments: Effect gene segment on virus property

    Res Vet Sci. 2012 Feb 13. [Epub ahead of print]
    Characterization of influenza A virus with nine segments: Effect gene segment on virus property.
    Wu R, Zhang X, Shao H, Luo Q, Yang J, Xu D.
    Source

    Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China; Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Wuhan 430064, Hubei, China; National Research Center for Veterinary Medicine, Luoyang Pulike Bio-engineering Co. Ltd., Luoyang 471003, Henan, China.
    Abstract

    The influenza A virus genome consists of eight segments of negative-strand RNA. In previous study, we generated a recombinant influenza virus with nine segments by reverse genetics. In present study, we evaluated characteristics of the recombinant influenza virus. The recombinant virus exhibited similar property to wild-type virus on virion morphology, virion composition, plaque phenotype and other aspects. Whereas, the recombinant virus propagated to lower titers than did wild-type virus in cells and mice, and there was decreased protein level and vRNA incorporation in the recombinant virions compared to wild-type H9N2 virions. Our results indicated that influenza A virus with eight segments exhibits more advantages than the virus with nine segments.

    Copyright © 2012. Published by Elsevier India Pvt Ltd.

    PMID:
    22336350
    [PubMed - as supplied by publisher]

    http://www.ncbi.nlm.nih.gov/pubmed/22336350
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