Once upon a time, there was a little Mingus working in a french-canadian lab somewhere in the north of north-america...
Some year before he started to analyse the SRRPvirus sequence data for this lab, a error haved occur where a wrong sequence where mixed between two farm and this big mistake have led the lab to always do every sequencing in double check to be sure there is no more nasty mistake like that.
Most of the time the doubled sequence was always the same than the first one.
However, over the last two years where about an average 8 to 10 sequence are analysed each weeks, by three times the second sequence was not the same than the first one.
One could imagine the terror in the eye of the little young Mingus.
Was it a big big mistake or not , little Mingus was in doubt...
But he was sure he did'nt and then refuse to let it down
He had notice than theses two strain was very different of thoses he had sequenced for others customers and he would have been hard to mix them with those. He also notice that theses two different strains where once been present in farm of this customers
He did know that the PCR prossess that we perform before the sequencing is a processus that amplify the DNA fragment exponentially, so if there is a mix of strains only one of the two will eventually be detectable.
Even more that nasty sample was a pool of 5 individual samples.
So he did the sample twice again and found :
- one non-readable data;
- one sequence that was the same as one of the two first.
Little mingus could haved think the second sequence he had the first time was a wrong one but he was allerted there was maybe a evil and Elusive Dual-Infection.
So he did also the five samples indivially and found:
-one negative sample
-two samples with the first sequence;
-two others samples wich show the second samples sequence
Yes!, little Mingus was so exited, he haved so proved the original sample was a mix of two diferent strains while his pals where convinced he just did a big bad mistake.
He then explain all this story to the veterinarian who ask for this analisys.
Two month later the same thing happen but that time, little mingus has never been able to prove the dual infection so he send a report with only the consensus sequence...
Little Mingus was then convinced that Elusives Dual-Infection happen more often than we think but are so elusive that most of the time there is no way to prove it without doubt.
That's my story
Only the virus and the type of sample haved been changed.
Any coincidence with real life is simply analogic.
This is for entertainment only.
Some year before he started to analyse the SRRPvirus sequence data for this lab, a error haved occur where a wrong sequence where mixed between two farm and this big mistake have led the lab to always do every sequencing in double check to be sure there is no more nasty mistake like that.
Most of the time the doubled sequence was always the same than the first one.
However, over the last two years where about an average 8 to 10 sequence are analysed each weeks, by three times the second sequence was not the same than the first one.
One could imagine the terror in the eye of the little young Mingus.
Was it a big big mistake or not , little Mingus was in doubt...
But he was sure he did'nt and then refuse to let it down
He had notice than theses two strain was very different of thoses he had sequenced for others customers and he would have been hard to mix them with those. He also notice that theses two different strains where once been present in farm of this customers
He did know that the PCR prossess that we perform before the sequencing is a processus that amplify the DNA fragment exponentially, so if there is a mix of strains only one of the two will eventually be detectable.
Even more that nasty sample was a pool of 5 individual samples.
So he did the sample twice again and found :
- one non-readable data;
- one sequence that was the same as one of the two first.
Little mingus could haved think the second sequence he had the first time was a wrong one but he was allerted there was maybe a evil and Elusive Dual-Infection.
So he did also the five samples indivially and found:
-one negative sample
-two samples with the first sequence;
-two others samples wich show the second samples sequence
Yes!, little Mingus was so exited, he haved so proved the original sample was a mix of two diferent strains while his pals where convinced he just did a big bad mistake.
He then explain all this story to the veterinarian who ask for this analisys.
Two month later the same thing happen but that time, little mingus has never been able to prove the dual infection so he send a report with only the consensus sequence...
Little Mingus was then convinced that Elusives Dual-Infection happen more often than we think but are so elusive that most of the time there is no way to prove it without doubt.
That's my story
Only the virus and the type of sample haved been changed.
Any coincidence with real life is simply analogic.
This is for entertainment only.
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