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Epitope characterization of the protective monoclonal antibody VN04-2 shows broadly neutralizing activity against highly pathogenic H5N1
Angeline PC. Lim
, Steven KK. Wong , Annie HY. Chan , Conrad EZ. Chan , Eng Eong Ooi and Brendon J. Hanson
Virology Journal 2008, 5:80doi:10.1186/1743-422X-5-80
<table cellpadding="0" cellspacing="0"> <tbody> <tr> <td>
</td></tr><tr> <td>Published:</td> <td>11 July 2008</td> </tr> </tbody> </table>Abstract (provisional)
The monoclonal antibody VN04-2 was previously shown to protect mice against lethal A/Vietnam/1203/04 H5N1 virus challenge when administered pre- and post-infection. In this study, we characterized the binding requirements of this antibody using direct binding to hemagglutinin and neutralization assays with H5N1 virus-like particles (H5N1-VLP) of eight recent H5N1 strains representing the major mutations within the 140s antigenic loop. Binding was clade independent and 3 mutations within this antigenic region are required before escape is possible, suggesting that apart from the H5N1 viruses circulating in Indonesia, VN04-2 may provide protection against H5N1 viruses from all other regions.
The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production.
Angeline PC. Lim
, Steven KK. Wong , Annie HY. Chan , Conrad EZ. Chan , Eng Eong Ooi and Brendon J. Hanson Virology Journal 2008, 5:80doi:10.1186/1743-422X-5-80
<table cellpadding="0" cellspacing="0"> <tbody> <tr> <td>
</td></tr><tr> <td>Published:</td> <td>11 July 2008</td> </tr> </tbody> </table>Abstract (provisional)
The monoclonal antibody VN04-2 was previously shown to protect mice against lethal A/Vietnam/1203/04 H5N1 virus challenge when administered pre- and post-infection. In this study, we characterized the binding requirements of this antibody using direct binding to hemagglutinin and neutralization assays with H5N1 virus-like particles (H5N1-VLP) of eight recent H5N1 strains representing the major mutations within the 140s antigenic loop. Binding was clade independent and 3 mutations within this antigenic region are required before escape is possible, suggesting that apart from the H5N1 viruses circulating in Indonesia, VN04-2 may provide protection against H5N1 viruses from all other regions.
The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production.
