Ook Nederland en Frankrijk vinden fragmenten Schmallenbergvirus in sperma van stieren
Niet alleen onderzoekers in Duitsland, maar ook in Nederland en Franrijk melden de vondst van fragmenten (RNA) van het Schmallenberg virus in sperma van stieren.
In Frankrijk bleken de stieren nog 2 tot 3 maanden positief te blijven.
Nader onderzoek vindt nog plaats.
Date: Thu 20 Dec 2012
From: Wim van der Poel [edited]
wim.vanderpoel@wur.nl
Detection of Schmallenberg virus RNA in semen samples
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The Central Veterinary Institute (CVI) of Wageningen University and Research Centre in Lelystad, the Netherlands, and the French Agency for Food and Environmental and Occupational Health and Safety (ANSES) can confirm from their own observations the detection of Schmallenberg virus [SBV] RNA in semen samples, as reported by the Friedrich-Loeffler-Institut on 19 Dec 2012.
At CVI, 55 semen samples produced in 2012 by 8 seroconverting/viraemic bulls have been analysed using a real-time RT-PCR system developed by FLI and an RNA extraction method developed by CVI. In total, 3 samples produced by 2 different bulls tested positive.
At LNCR (National Laboratory for sanitary controls in breeding animals, France) together with ANSES, 904 semen samples produced in 2011 and 2012 by 160 seropositive bulls have been analysed using a real-time RT-PCR system developed by FLI and an RNA extraction method developed by LNCR. In total 26 samples produced by 2 different bulls were tested positive for 2 to 3 months.
Because of these findings, the institutes are currently performing in vitro and in vivo studies on SBV excretion in semen. These studies are supported by the European Union and the national governments.
In the meantime, to declare semen free of SBV, it is advised to test semen samples for the presence of SBV RNA using an approved RT-PCR and RNA extraction method, unless the semen was produced before 31 May 2011 or the bull was tested SBV antibody negative at least 28 days after production.
Prof Dr Wim H M van der Poel (Department of Virology)
Dr Ruth Bouwstra, Dr Johan Bongers (Department of Diagnostics)
Central Veterinary Institute of Wageningen University and Research Centre, Lelystad, the Netherlands.
Dr Stephan Zientara, French Agency for Food and Environmental and Occupational Health and Safety;
Dr Claire Ponsart, National Laboratory for sanitary controls in breeding animals, France.
More: ProMED-mail
From: Wim van der Poel [edited]
wim.vanderpoel@wur.nl
Detection of Schmallenberg virus RNA in semen samples
-----------------------------------------------------
The Central Veterinary Institute (CVI) of Wageningen University and Research Centre in Lelystad, the Netherlands, and the French Agency for Food and Environmental and Occupational Health and Safety (ANSES) can confirm from their own observations the detection of Schmallenberg virus [SBV] RNA in semen samples, as reported by the Friedrich-Loeffler-Institut on 19 Dec 2012.
At CVI, 55 semen samples produced in 2012 by 8 seroconverting/viraemic bulls have been analysed using a real-time RT-PCR system developed by FLI and an RNA extraction method developed by CVI. In total, 3 samples produced by 2 different bulls tested positive.
At LNCR (National Laboratory for sanitary controls in breeding animals, France) together with ANSES, 904 semen samples produced in 2011 and 2012 by 160 seropositive bulls have been analysed using a real-time RT-PCR system developed by FLI and an RNA extraction method developed by LNCR. In total 26 samples produced by 2 different bulls were tested positive for 2 to 3 months.
Because of these findings, the institutes are currently performing in vitro and in vivo studies on SBV excretion in semen. These studies are supported by the European Union and the national governments.
In the meantime, to declare semen free of SBV, it is advised to test semen samples for the presence of SBV RNA using an approved RT-PCR and RNA extraction method, unless the semen was produced before 31 May 2011 or the bull was tested SBV antibody negative at least 28 days after production.
Prof Dr Wim H M van der Poel (Department of Virology)
Dr Ruth Bouwstra, Dr Johan Bongers (Department of Diagnostics)
Central Veterinary Institute of Wageningen University and Research Centre, Lelystad, the Netherlands.
Dr Stephan Zientara, French Agency for Food and Environmental and Occupational Health and Safety;
Dr Claire Ponsart, National Laboratory for sanitary controls in breeding animals, France.
More: ProMED-mail
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