//TEMPORARY THREAD//:RESEARCH ABSTRACT:

[Giuseppe]

J Clin Microbiol. 2008 Mar 5 [Epub ahead of print]
Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds.

Xing Z, Cardona C, Dao P, Crossley B, Hietala S, Boyce W.
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; California Animal Health and Food Safety Laboratory; Wildlife Health Center.

We report a failure of the current real-time RT-PCR H7 subtyping protocol, used in national avian influenza surveillance programs.

Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses.

The protocol, designed originally for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.

PMID: 18322070 [PubMed - as supplied by publisher]

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[gsgs]

Re: //TEMPORARY THREAD//:RESEARCH ABSTRACT:

here is the link:

http://www.ncbi.nlm.nih.gov/pubmed/18322070?ordinalpos=22&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

full article $15 for one day.


niman's doubts on the reliability of the 300000 H5N1-negative
birds comes to mind