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machinetranslated
26/10/2010
Circulating avian influenza virus detected in wild birds in Catalonia
Virus Research magazine recently published a study in which they
CReSA researchers involved and has shown that viruses
avian influenza circulating in wild birds in Catalonia are
phylogenetically related to viruses Eurasians. This study is
pioneer in the detection and study of different virus subtypes
Avian flu in Spain from their natural hosts, wild birds.
This study commissioned by CReSA by the Department of Agriculture,
Food and Rural Action (DAR) in collaboration with the Department of Medi
Environment and Housing (DMAiH), is part of influenza surveillance program
influenza in wild birds.
It is recalled that since the outbreak of bird flu
H5N1, the virus has caused a great importance with regard to health
public and animal health and that is why throughout the world have established
monitoring programs of this type to control the virus.
The results of this study of 3 years of monitoring show that there is
found no highly pathogenic virus in wild birds in Catalonia
although viruses have been detected H5 and H7 viruses of high potential
pathogenic in poultry production, and avian influenza viruses
circulating in our country belong to a phylogenetic group Eurasian
clearly differentiated from the American.
This correlates well
with the migratory routes of wild birds passing through Catalonia, routes
Northern Europe and Africa. I must say that this paper provides new
ecological information of the avian influenza virus in the Mediterranean Area
Western.
The results of this article can be found in the following publication:
"Influenza A virus subtypes in wild birds in North-Eastern Spain (Catalonia)."
Influenza A virus subtypes in wild birds in North-Eastern Spain (Catalonia).
Abstract
Since the spread of H5N1 highly pathogenic avian influenza virus in 2005, many surveillance programmes have been initiated in poultry and wild birds worldwide.
This study describes for the first time the detection of different subtypes of avian influenza viruses (AIV) in wild birds in the West Mediterranean area (Catalonia, North-Eastern Spain).
During a 3-year period (from mid-2006 to mid-2009), 1374 birds from 16 different families were examined, and a total of 62 AIV were detected by means of a real-time reverse transcriptase PCR assay. AIV were more frequently detected in Anatidae, Phoenicopteridae, Rallidae and Laridae families.
Of the 62 positive samples, 28 AIV could be isolated in embryonated eggs. All isolates were subtyped by haemagglutinin and neuraminidase inhibition techniques and 10 different haemagglutinins (HA) and 7 neuraminidases (NA) were found in 13 different subtype combinations. The most common combinations were H4N6 (22.2%) and H1N1 (18.5%). The HA and NA gene sequences of different AIV subtypes were compared and aligned with those available AIV strains from genome databases.
Our studies on AIV phylogenetic analysis revealed that all AIV genes sequenced from wild birds in North-Eastern Spain clustered within Eurasian avian clades, including the sequences of H8, N4 and N5 genes analyzed for the first time in Europe. The results contribute to the understanding of AIV in the Mediterranean area and in Europe.
PubMED
26/10/2010
Circulating avian influenza virus detected in wild birds in Catalonia
Virus Research magazine recently published a study in which they
CReSA researchers involved and has shown that viruses
avian influenza circulating in wild birds in Catalonia are
phylogenetically related to viruses Eurasians. This study is
pioneer in the detection and study of different virus subtypes
Avian flu in Spain from their natural hosts, wild birds.
This study commissioned by CReSA by the Department of Agriculture,
Food and Rural Action (DAR) in collaboration with the Department of Medi
Environment and Housing (DMAiH), is part of influenza surveillance program
influenza in wild birds.
It is recalled that since the outbreak of bird flu
H5N1, the virus has caused a great importance with regard to health
public and animal health and that is why throughout the world have established
monitoring programs of this type to control the virus.
The results of this study of 3 years of monitoring show that there is
found no highly pathogenic virus in wild birds in Catalonia
although viruses have been detected H5 and H7 viruses of high potential
pathogenic in poultry production, and avian influenza viruses
circulating in our country belong to a phylogenetic group Eurasian
clearly differentiated from the American.
This correlates well
with the migratory routes of wild birds passing through Catalonia, routes
Northern Europe and Africa. I must say that this paper provides new
ecological information of the avian influenza virus in the Mediterranean Area
Western.
The results of this article can be found in the following publication:
"Influenza A virus subtypes in wild birds in North-Eastern Spain (Catalonia)."
Influenza A virus subtypes in wild birds in North-Eastern Spain (Catalonia).
Abstract
Since the spread of H5N1 highly pathogenic avian influenza virus in 2005, many surveillance programmes have been initiated in poultry and wild birds worldwide.
This study describes for the first time the detection of different subtypes of avian influenza viruses (AIV) in wild birds in the West Mediterranean area (Catalonia, North-Eastern Spain).
During a 3-year period (from mid-2006 to mid-2009), 1374 birds from 16 different families were examined, and a total of 62 AIV were detected by means of a real-time reverse transcriptase PCR assay. AIV were more frequently detected in Anatidae, Phoenicopteridae, Rallidae and Laridae families.
Of the 62 positive samples, 28 AIV could be isolated in embryonated eggs. All isolates were subtyped by haemagglutinin and neuraminidase inhibition techniques and 10 different haemagglutinins (HA) and 7 neuraminidases (NA) were found in 13 different subtype combinations. The most common combinations were H4N6 (22.2%) and H1N1 (18.5%). The HA and NA gene sequences of different AIV subtypes were compared and aligned with those available AIV strains from genome databases.
Our studies on AIV phylogenetic analysis revealed that all AIV genes sequenced from wild birds in North-Eastern Spain clustered within Eurasian avian clades, including the sequences of H8, N4 and N5 genes analyzed for the first time in Europe. The results contribute to the understanding of AIV in the Mediterranean area and in Europe.
PubMED