Still no H5N8 sequences at GenBank

[gsgs]

3 weeks now that 5 segments were found that are in America for the first time
since >50 years, some probably since >100.
And it had already reassorted with American influenza.
Enhancing the American and thus worldwide influenza genetical reservoir.
That's the way how pandemics are created.

Still no sequences at genbank.

7 weeks now since H5N8 appeared in Europe mysteriously with no trace
between Europe and SE-Asia.With great impact for the European poultry
industry and private fowl keepers, as well as pandemic potential according
to the latest paper by Li,Wang(2),Gu,Zhu,Hao,Gao,Sun,Hu(2),Liu(2).

Still no sequences at genbank.

Remember how long it took with H7N9 in 2013 (2013/04/08)

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http://www.ncbi.nlm.nih.gov/nuccore/KC853766

https://flutrackers.com/forum/forum/...7n9#post588426:
On March 31, 2013, the Centre for Health Protection (CHP) of the Department of Health
of Hong Kong and the Chinese National Health and Family Planning Commission notified
the World Health Organization of three confirmed human cases of influenza A (H7N9) in
Shanghai and Anhui (illness onset between February 19 and March 15, 2013).[30]
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and pandemic H1N1 in 20092009/04/27)

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https://flutrackers.com/forum/forum/genetic-tracking-scientfic-analysis-of-pandemic-influenza-other-diseases/genetic-analysis-of-swine-influenza-a-h1n1-2009/48070-sequences-at-genbank?t=101112&page=1

[this site doesn't load with my old browser]
the sequences had been published at genbank:

http://www.ncbi.nlm.nih.gov/genomes/FLU/SwineFlu.html

40 sequences on 27.April
34 sequences on 28.April
On April 17, 2009, CDC and the California Department of Public Health determined that
two cases of febrile respiratory illness occurring in children who reside in adjacent
counties in southern California were caused by infection with a swine influenza A (H1N1) virus.
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[gsgs]

H5N1,1997
isolated 1997/05/19
paper submitted 1997/09/26
paper accepted 1997/12/09
published 1998/01/16
sequences were available in June 1997 already and were compared with sequences
from EMBL databases at that time

press release St.Jude, Oct.1997:
http://www.eurekalert.org/pub_releas...ISD-081097.php
article published 1997/10/09 in "Nature"

http://www.cdc.gov/mmwr/preview/mmwrhtml/00050775.htm

The first known case of human infection with influenza A(H5N1) occurred in a 3-year-old boy
who died from respiratory failure in May 1997 (1).
Of the 15 remaining confirmed cases, five persons had onset of illness in November
and 10 in December;

http://www.cdc.gov/mmwr/preview/mmwrhtml/00050459.htm

In Hong Kong, the virus initially was identified as influenza type A, but the subtype could not be
determined using standard reagents. By August, CDC; the National Influenza Center, Rotterdam,
the Netherlands; and the National Institute for Medical Research, London, United Kingdom,
had independently identified the virus as influenza A(H5N1)

Patient 1. On May 9, 1997, a previously healthy 3-year-old boy developed fever
Patient 2. On November 6, a 2-year-old boy with a congenital heart disease developed high fever
Patient 3. On November 20, a previously healthy 13-year-old girl developed fever
Patient 4. On November 24, a previously healthy 54-year-old man developed fever
Patient 5. On December 4, a 24-year-old woman developed fever
Patient 6. On December 7, a 5-year-old girl developed fever
Patient 7. On December 12, a 2-year-old boy developed fever

http://www.ncbi.nlm.nih.gov/nuccore/AF028709

released 1998/02/11
(A/Hong Kong/156/97(H5N1))
Submitted (07-OCT-1997)

http://www.ncbi.nlm.nih.gov/pubmed/9482438

Lancet, Volume 351, No. 9101, p472–477, 14 February 1998
Erratum in Lancet 1998 Apr 25;351(9111):1292.
In May, 1997, a 3-year-old boy ...
METHODS:
We carried out an antigenic and molecular comparison of the influenza A H5N1 virus isolated
from the boy with one of the viruses isolated from outbreaks of avian influenza by
haemagglutination-inhibition and neuraminidase-inhibition assays and nucleotide sequence analysis.
FINDINGS:
Differences were observed in the antigenic reactivities of the viruses by the haemagglutination-
inhibition assay. However, nucleotide sequence analysis of all gene segments revealed that
the human virus A/Hong Kong/156/97 was genetically closely related to the avian
A/chicken/Hong Kong/258/97.

http://www.ncbi.nlm.nih.gov/pubmed/9430591

Science. 1998 Jan 16;279(5349):393-6.
The nucleotide sequences have been deposited in GenBank (accession
numbers AF036356 through AF036363).

Note added in proof: Since the submission
of this report, there have been 12 additional
confirmed human cases of influenza A
(H5N1) infections in Hong Kong, include-
ing three fatalities. Sequence analysis of the
genes of six of the isolates revealed that all
of the genes are of avian origin and are
closely related to each other. The HA gene
codes for a multiple basic amino acid inser-
tion upstream of the cleavage site, associat-
ed with highly pathogenic avian influenza
viruses and identical to that seen in the
A/Hong Kong/156/97 virus.


Table 1. Molecular analysis of the gene segments of the A/Hong Kong/156/97 virus.
Nucleotide sequences were compared, and identity was determined by FASTA
(Wisconsin Package, version 9.0) searches of GenBank and European Molecular Biology
Laboratory (EMBL) databases as of August and June 1997, respectively.

[gsgs]

Russia usually submits to genbank, not gisaid !

http://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_refer=MapFullEventReport&reportid=16826

Belaya Gora, Abyjsky, RESPUBLIKA SAKHA(YAKUTIYA), NO-Sibiria, 25/09/2014
Eurasian Wigeon = Anas penelope = Pfeifente = Smient
full genom sequencing will be done

[gsgs]

it's grotesk.
The most discussed question about H5N8 in Europe is, how it spreads - wild birds or cars.
How it may enter the poultry farms despite all the security measures.

What measures should be taken, culling, trade restrictions, onstalling ?
The sequences would help to decide that.

We have these groups (DE,NL) who think it's not the wild birds, they ignore the sequences.
The official institutes say it's the wild birds but can't argue in the press with sequences
because those mustn't be published because of the GISAID conditions.
I think FLI(DE),CXXX(NL),DEFRA(UK) are afraid to mention the sequences to the press
because they are afraid that reporters may ask then how to verify it, why they are not
published.