Announcement

**makoto** · January 23, 2011, 07:04 AM

Japan - Wild birds infected with H5N1 in Hokkaido

23 Jan., ministry of the environment government of japan "MOE" announced
Hokkaido prefecture announced http://www.agri.pref.hokkaido.jp/kaho/hpai/230123%20kankyousyou-press.pdf

<iframe width="200" height="170" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="http://maps.google.ch/maps?ie=UTF8&t=h&ll=43.04606,145.006485&am p;spn=0.042653,0.068321&z=12&output=embed" ></iframe><br /><small><a href="http://maps.google.ch/maps?ie=UTF8&t=h&ll=43.04606,145.006485&am p;spn=0.042653,0.068321&z=12&source=embed" style="color:#0000FF;text-align:left">hichirippu pond</a></small> Hamanaka town, Hokkaido prefecture

12 to 21 Jan.,
unhealthy 2 wild birds and 13 dead birds at hichirippu or maruyamachirippu
*1 whooper swan was positive by simple test → the 1st H5N1 case
*9 wild birds were negative by simple test
*simple test for 5 wild birds were impossible
10 bird' specimens transferred to Hokkaido university

22 Jan.,
Hokkaido university detected H5N1 subtype from a whooper swan → the 1st H5N1 case

23 Jan.,
Hokkaido university detected H5N1 subtype from 4 wild birds
*4 wild birds were negative by simple test
4 wild birds; 2 whooper swans, 1 duck(greater scaups) and 1 northern pintail(anas acuta)

discovery date;
whooper swans: 12 and 18 Jan
duck(greater scaups): 17 Jan
northern pintail: 19 Jan

these viral genes are in complete agreement with the 1st case

Hokkaido prefecture inspected 4 farms within 10 km radius from the 1st case
4 farms had no problem, these farms have a total of 80 birds

an unhealthy whooper swan
ANN news
disinfecting in the passage of wildlife sanctuary
mainichi jp

Northern Pintail(anas acuta) http://en.wikipedia.org/wiki/Northern_Pintail
Greater Scaup (Aythya marila) http://en.wikipedia.org/wiki/Greater_Scaup
jiji com http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
47 news http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
sankei news http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
ann news http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
yomiuri online http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
mainichi jp http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2

from lake Akkeshi & Bekanbeushi marsh live camera
14:01, 23 Jan

collection of droppings at Mochirippu pond, 24 Jan
mainichi jp

**makoto** · January 28, 2011, 07:32 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido

28 Jan., Hokkaido prefecture announced

<iframe width="200" height="200" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="http://maps.google.co.jp/maps?hl=fr&ie=UTF8&t=h&brcurrent=3,0x3 4674e0fd77f192f:0xf54275d47c665244,1&ll=43.065 878,145.104675&spn=0.050164,0.068321&z=12& amp;output=embed"></iframe><br /><small><a href="http://maps.google.co.jp/maps?hl=fr&ie=UTF8&t=h&brcurrent=3,0x3 4674e0fd77f192f:0xf54275d47c665244,1&ll=43.065 878,145.104675&spn=0.050164,0.068321&z=12& amp;source=embed" style="color:#0000FF;text-align:left">biwase bay(side)</a></small> Shinkawa, Hamanaka town, Hokkaido prefecture

an unhealthy young whooper swan was positive by simple test
specimens transfers to Hokkaido university

yomiuri online http://www.excite-webtl.jp/world/english/web/?wb_url=http%3A%2F%2Fwww.yomiuri.co.jp%2Fnational% 2Fnews%2F20110128-OYT1T00852.htm&wb_lp=JAEN&wb_dis=2
asahi com http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2

**makoto** · February 1, 2011, 05:30 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

1st Feb., Hokkaido prefecture announced http://www.agri.pref.hokkaido.jp/kah...syou-press.pdf http://translate.google.co.jp/translate?hl=ja&sl=ja&tl=en&u=http%3A%2F%2Fwww.pre f.hokkaido.lg.jp%2Fks%2Fskn%2Ftyuijikou221029

H5N1 subtype was detected from Whooper Swan

Hokkaido University confirmed H5N1 sybtype on 1st Feb
*this Whooper Swan is 28 Jan case↑

sankei news http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
yomiuri online http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2

**gsgs** · February 3, 2011, 05:22 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

A/Whooper Swan/Hokaido/4/2011/01/(H5N1)

was released at genbank.

not the Qinghai strain, but basically the Fujian-strain
similar to A/Whooper Swan/Mongolia/6/2009/
(8,8,4,6,11,7,8,3 promille different in the 8 segments)

with a slightly different
PA ("Index China 2003")
and HA ("Guangxi/2005")

genotype V ?

Code:

 29 >A/whooper swan/Hokkaido/4/2011 2011-01-15   2280 2274 2151 1704 1497 1350  982  823   
  1:---, 36,---,---,---,---,---,---   A/avian/Index-b/1996(H5N1)                     3 2274    3    3    3    3    3    3
  2:---, 36,---,---,---,---,---,---   A/avian/Index/1996(H5N1)                       3 2274    3    3    3    3    3    3
  3: 41, 36, 38, 55, 30, 53, 24, 25   Index China03                               2280 2274 2151 1707 1497 1350  982  823
  4: 39, 37, 62, 55, 26, 51, 23, 23   A/Index-se9agbi.26                          2280 2274 2151 1698 1497 1350  982  823
  5: 47, 35, 67, 58, 27, 48, 26, 31   A/avian/Indonesia-Index(H5N1)               2280 2274 2151 1707 1497 1350  982  823
  6: 79, 81, 63, 72, 80, 66, 45,288   A/Gs/Guangdong/1/1996,GsGd                  2280 2274 2151 1707 1497 1410  982  838
  7:150,107,101, 75, 87,123, 92,104   A/Ck/HK/220/1997,GsGd                       2015 2274 2148 1707 1497 1353  982  838
  8:145,106,101, 72, 89,124, 93, 94   A/HK/156/1997,GsGd                          2248 2274 2148 1707 1497 1353  982  838
  9: 49, 47, 62, 65, 94, 65, 50, 37   A/Silky Ck/HK/SF189/2001,A                  2207 2274 2147 1674 1497 1350  982  823
 10: 53, 48, 65, 66, 96, 92, 35, 40   A/Ck/HK/YU562/2001,B                        2279 2273 2150 1705 1496 1409  982  823
 11: 50, 78, 62, 62, 82, 62, 31, 37   A/Dk/HK/2986.1/2000,C                       2277 2253 2148 1704 1494 1407  982  823
 12: 50, 78, 65, 66,114, 65, 32, 36   A/Ck/HK/FY150/2001,D                        2279 2273 2150 1705 1496 1409  982  823
 13: 50, 79, 64, 67, 33, 63, 31, 38   A/Ck/HK/715.5/2001,E                        2226 2274 2147 1668 1497 1410  982  823
 14: 94, 44, 69, 44, 29, 54, 29, 54   A/Gs/Guangxi/345/2005,G                     2277 2271 2148 1695 1454 1323  982  811
 15: 46, 42, 39, 60, 36, 57, 24, 27   A/Ck/ST/4231/2003,V                         2277 2274 2130 1697 1497 1350  981  823
 16: 89, 41, 65, 70, 83, 69, 26, 43   A/teal/China/2978.1/2002,W                  2280 2274 2130 1636 1497 1367  966  772
 17: 86,103, 85, 69, 38, 71, 53, 61   A/Gf/HK/38/2002,X0                          2208 2154 2151 1667 1497 1075  962  838
 18:133,102, 87, 70, 37, 64, 51, 61   A/Ck/HK/31.2/2002,X1                        2258 2273 2151 1701 1497 1350  982  838
 19: 71,100, 71, 72, 71, 69, 93, 60   A/Ck/HK/37.4/2002,X2                        2271 2274 2081 1701 1497 1350  982  838
 20: 65, 91, 69, 69, 32, 64, 51, 66   A/SCk/HK/YU100/2002,X3                      2274 2272 2151 1701 1497 1350  982  838
 21: 54, 38, 64, 68, 27, 56, 37, 25   A/Ck/HK/96.1/2002,Y                         2277 2261 2148 1700 1497 1350  982  823
 22: 43, 40, 64, 63, 28, 56, 24, 26   A/Ck/HK/YU22/2002,Z                         2280 2274 2151 1679 1497 1350  982  823
 23: 46, 39, 66, 56, 28, 73, 29, 27   A/HK/212/2003,Z+                            2280 2274 2139 1653 1497 1407  982  823
 24: 82,124,100,155, 98,140, 77, 75   A/Ck/Scotland/1959(H5N1)                    2280 2274 2151 1695 1497 1410  982  838
 25: 40, 47, 71, 61, 34, 56, 31, 37   Index Qinghai                               2280 2274 2151 1707 1497 1350  982  823
 26: 46, 40, 68, 59, 31, 60, 28, 32   Index Vietnam                               2280 2274 2151 1707 1497 1350  982  823
 27: 39, 28, 45, 55, 25, 37, 21, 16   Index Fujian                                2330 2278 2163 1709 1497 1411 1011  838
 28: 40, 47, 71, 61, 34, 56, 31, 37   ######A/Index/Qinghai/05,02/10              2280 2274 2151 1707 1497 1350  982  823
 29:  0,  0,  0,  0,  0,  0,  0,  0   A/whooper swan/Hokkaido/4/2011 2011-01-15   2280 2274 2151 1704 1497 1350  982  823

**makoto** · February 3, 2011, 09:47 PM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

3rd Feb., Hokkaido prefecture announced

dead young whooper swan was positive by simple test

hichirippu pond, Hamanaka town, Hokkaido prefecture

bird inspector found young whooper swan
whooper swan was positive by simple test
specimens transfers to Hokkaido university

doshin web http://www.excite-webtl.jp/world/english/web/?wb_url=http%3A%2F%2Fwww.hokkaido-np.co.jp%2Fnews%2Fagriculture%2F271592.html&wb_lp= JAEN&wb_dis=2

**gsgs** · February 4, 2011, 12:59 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

Influenza A virus (A/whooper swan/Hokkaido/4/2011(H5N1)) viral cRNA, s - Nucleotide - NCBI

http://www.ncbi.nlm.nih.gov/nuccore/AB610972?

> /collection_date="2011"

no day or month given, but it must be January ;-)

> (A/whooper swan/Hokkaido/4/2011(H5N1))

so I assume they have at least 3 others (Hokkaido/1,Hokkaido/2,Hokkaido/3) just at Hokkaido University

REFERENCE 1
AUTHORS Kida,H., Sakoda,Y. and Okamatsu,M.
TITLE Characterization of H5N1 influenza viruses isolated from waterbirds
in Japan in 2010-2011
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1743)
AUTHORS Makiko,J., Kida,H., Sakoda,Y. and Okamatsu,M.
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2011) Contact:Jizou Makiko Hokkaido University,
Graduate School of Veterinary Medicine; kita-ku, kita18 nishi9,
Sapporo, Hokkaido 060-0818, Japan

**makoto** · February 8, 2011, 09:04 AM

Re: Japan - wild birds infected with H5N1 in Hokkaido confirmed

8 Feb., Hokkaido prefecture announced http://translate.google.co.jp/translate?hl=ja&sl=ja&tl=en&u=http%3A%2F%2Fwww.pre f.hokkaido.lg.jp%2Fks%2Fskn%2Ftyuijikou221029

H5N1 subtype was detected from Whooper Swan

Hokkaido University confirmed H5N1 sybtype on 8 Feb
*this Whooper Swan is 3 Feb case

sankei news http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
__________

8 Feb., MOE announced

greater scaup was positive by simple test

Hamanaka town, Hokkaido prefecture

7 Feb.,
dead greater scaup at Hamanaka town in the evening

simple test was positive
specimens transfers to Hokkaido university

sankei news http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2

from lake Akkeshi & Bekanbeushi marsh live camera
15:01, 8 Feb

**gsgs** · February 9, 2011, 03:04 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

looking at the picture ... how would it transmit from swan to swan ?

Breathing,sneezing,coughing as with humans ?

water,feathers, "dabblong" as with mallards ?

cleaning the feathers or preening with the bill
even neighbour or relatives - preening ?

eating,drinking contaminated water/food

laying on the sand where a sick swan was before
lung2bill2feather2sand2feather2bill2lung

or direct contact, standing close together, feather2feather

**sharon sanders** · February 9, 2011, 10:04 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

It is probably excrement to ground to mouth transmission. Ducks are very dirty animals and expel a lot of loose diarrhea type stool.

**gsgs** · February 9, 2011, 11:27 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

swans are not ducks.

We know, it's mainly waterborne with mallards

but then it mutates away from the index(mallards)
then it becomes airborne (mammals, poultry?,swans?, gulls?)

**makoto** · February 14, 2011, 07:14 AM

Re: Japan - Whooper Swan infected with H5N1 in Hokkaido confirmed

14 Feb., Hokkaido prefecture announced http://translate.google.ch/translate...syou-press.pdf http://translate.google.co.jp/transl...yuijikou221029

H5N1 subtype was detected from greater scaup

Hokkaido University confirmed H5N1 sybtype on 14 Feb
*this greater scaup is 7 Feb case

jiji com http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2
yomiuri online http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2

from lake Akkeshi & Bekanbeushi marsh live camera
13:01, 13 Feb

Attached Files

**makoto** · February 17, 2011, 07:44 AM

Re: Japan - Whooper Swan and duck infected with H5N1 in Hokkaido prefecture

17 Feb., Hokkaido prefecture announced

dead whooper swan was positive by simple test

<iframe width="150" height="150" frameborder="0" scrolling="no" marginheight="0" marginwidth="0" src="http://maps.google.ch/maps?hl=fr&ie=UTF8&t=h&ll=43.056221,14 4.83963&spn=0.018815,0.025578&z=13&out put=embed"></iframe><br /><small><a href="http://maps.google.ch/maps?hl=fr&ie=UTF8&t=h&ll=43.056221,14 4.83963&spn=0.018815,0.025578&z=13&sou rce=embed" style="color:#0000FF;text-align:left">ponto, akkeshi town</a></small> Hokkaido prefecture

17 Feb.,
found dead young whooper swan
whooper swan was positive by simple test

specimens transfers to Hokkaido university

sankei news http://www.excite-webtl.jp/world/english/web/?wb_url=http%3A%2F%2Fsankei.jp.msn.com%2Flife%2Fne ws%2F110217%2Fbdy11021721030007-n1.htm&wb_lp=JAEN&wb_dis=2

from lake Akkeshi & Bekanbeushi marsh live camera
09:01, 17 Feb

**makoto** · February 21, 2011, 02:50 AM

Re: Japan - Whooper Swan and duck infected with H5N1 in Hokkaido prefecture

21 Feb., Hokkaido prefecture announced http://translate.google.co.jp/translate?hl=ja&sl=ja&tl=en&u=http%3A%2F%2Fwww.pre f.hokkaido.lg.jp%2Fks%2Fskn%2Ftyuijikou221029 http://www.agri.pref.hokkaido.jp/kah...syou-press.pdf

H5N1 subtype was detected from whooper swan

Hokkaido University confirmed H5N1 sybtype on 21 Feb
*this swan is 17 Feb case

jiji com http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2

from lake Akkeshi & Bekanbeushi marsh live camera
17:01, 20 Feb

Attached Files

**makoto** · February 22, 2011, 07:09 PM

Re: Japan - Whooper Swan and duck infected with H5N1 in Hokkaido prefecture

22 Feb., MOE announced
Hokkaido prefecture announced http://www.agri.pref.hokkaido.jp/kah...2-10-press.pdf http://translate.google.co.jp/translate?hl=ja&sl=ja&tl=en&u=http%3A%2F%2Fwww.pre f.hokkaido.lg.jp%2Fks%2Fskn%2Ftyuijikou221029

dead whooper swan was positive by simple test

Hamanaka town, Hokkaido prefecture

20(21?) Feb.,
found whooper swan
whooper swan was positive by simple test

MOE(Kushiro Office in Hokkaido) confirms H5N1 case by simple test result from now on

mainichi jp http://www.excite-webtl.jp/world/eng...=JAEN&wb_dis=2

from lake Akkeshi & Bekanbeushi marsh live camera
13:01, 22 Feb

Attached Files

Announcement

Japan - Whooper Swan and duck infected with H5N1 in Hokkaido prefecture

Japan - Whooper Swan and duck infected with H5N1 in Hokkaido prefecture

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