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Proc Natl Acad Sci USA. Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes
Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes (PNAS, abstract, edited)
[Source: PNAS, full text: <cite cite="http://www.pnas.org/content/107/42/18132.short?rss=1">Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes ? PNAS</cite>. Abstract, edited.]
Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes
1. Patrick Veiga a,b, 2. Carey Ann Gallini a, 3. Chlo? Beal b, 4. Monia Michaud a, 5. Mary L. Delaney c, 6. Andrea DuBois c, 7. Artem Khlebnikov b,d, 8. Johan E.T. van Hylckama Vlieg b, 9. Shivesh Punit a,1, 10. Jonathan N. Glickman c,e,2, 11. Andrew Onderdonk c,e, 12. Laurie H. Glimcher a,d,e,f, and 13. Wendy S. Garrett a,e,g,3
Author Affiliations
1. aHarvard School of Public Health, Boston, MA 02115;
2. bDanone Research, 91767 Palaiseau, France
3. cBrigham and Women's Hospital, Boston, MA 02115;
4. dDannon Company Inc., White Plains, NY 10603;
5. eHarvard Medical School, Boston, MA 02115;
6. fRagon Institute of MGH, MIT and Harvard, Charlestown, MA 02129; and
7. gDana Farber Cancer Institute, Boston, MA 02115
* ↵1Present address: Department of Cell and Developmental Biology, University of Southern California, Health Sciences Campus, Los Angeles, CA 90089.
* ↵2Present address: GI Pathology, Boston Caris Diagnostics, Newton, MA 02464.
1. Edited by Peter K. Vogt, The Scripps Research Institute, La Jolla, CA, and approved September 8, 2010 (received for review August 5, 2010)
Abstract
Intestinal health requires the coexistence of eukaryotic self with the gut microbiota and dysregulated host-microbial interactions can result in intestinal inflammation. Here, we show that colitis improved in T-bet−/−Rag2−/− mice that consumed a fermented milk product containing Bifidobacterium animalis subsp. lactis DN-173 010 strain. A decrease in cecal pH and alterations in short chain fatty acid profiles occurred with consumption, and there were concomitant increases in the abundance of select lactate-consuming and butyrate-producing bacteria. These metabolic shifts created a nonpermissive environment for the Enterobacteriaceae recently identified as colitogenic in a T-bet−/−Rag2−/− ulcerative colitis mouse model. In addition, 16S rRNA-based analysis of the T-bet−/−Rag2−/−fecal microbiota suggest that the structure of the endogenous gut microbiota played a key role in shaping the host response to the bacterial strains studied herein. We have identified features of the gut microbiota, at the membership and functional level, associated with response to this B. lactis-containing fermented milk product, and therefore this model provides a framework for evaluating and optimizing probiotic-based functional foods.
* Enterobacteriaceae
* intestinal inflammation
* microbiota
* probiotics
* colitis
Footnotes
* 3 To whom correspondence should be addressed. E-mail: wgarrett@hsph.harvard.edu.
* Author contributions: P.V., A.K., J.E.T.v.H.V., A.O., L.H.G., and W.S.G. designed research; P.V., C.A.G., C.B., M.M., M.L.D., A.D., S.P., and W.S.G. performed research; P.V., C.A.G., C.B., M.M., M.L.D., A.D., J.E.T.v.H.V., J.N.G., A.O., and W.S.G. analyzed data; and P.V. and W.S.G. wrote the paper.
* Conflict of interest statement: L.H.G. is a member of the Board of Directors of and holds equity in the Bristol Myers Squibb Corporation. P.V., C.B., A.K., and J.E.T.v.H.V. are employees of and hold equity in Groupe Danone.
* Data deposition: The 454 pyrosequencing reads reported in this paper have been deposited in the NCBI Short Read Archive (accession no. SRX025834.5).
* This article is a PNAS Direct Submission.
* This article contains supporting information online at https://www.pnas.org/lookup/suppl/do...DCSupplemental.
Freely available online through the PNAS open access option.
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[Source: PNAS, full text: <cite cite="http://www.pnas.org/content/107/42/18132.short?rss=1">Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes ? PNAS</cite>. Abstract, edited.]
Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes
1. Patrick Veiga a,b, 2. Carey Ann Gallini a, 3. Chlo? Beal b, 4. Monia Michaud a, 5. Mary L. Delaney c, 6. Andrea DuBois c, 7. Artem Khlebnikov b,d, 8. Johan E.T. van Hylckama Vlieg b, 9. Shivesh Punit a,1, 10. Jonathan N. Glickman c,e,2, 11. Andrew Onderdonk c,e, 12. Laurie H. Glimcher a,d,e,f, and 13. Wendy S. Garrett a,e,g,3
Author Affiliations
1. aHarvard School of Public Health, Boston, MA 02115;
2. bDanone Research, 91767 Palaiseau, France
3. cBrigham and Women's Hospital, Boston, MA 02115;
4. dDannon Company Inc., White Plains, NY 10603;
5. eHarvard Medical School, Boston, MA 02115;
6. fRagon Institute of MGH, MIT and Harvard, Charlestown, MA 02129; and
7. gDana Farber Cancer Institute, Boston, MA 02115
* ↵1Present address: Department of Cell and Developmental Biology, University of Southern California, Health Sciences Campus, Los Angeles, CA 90089.
* ↵2Present address: GI Pathology, Boston Caris Diagnostics, Newton, MA 02464.
1. Edited by Peter K. Vogt, The Scripps Research Institute, La Jolla, CA, and approved September 8, 2010 (received for review August 5, 2010)
Abstract
Intestinal health requires the coexistence of eukaryotic self with the gut microbiota and dysregulated host-microbial interactions can result in intestinal inflammation. Here, we show that colitis improved in T-bet−/−Rag2−/− mice that consumed a fermented milk product containing Bifidobacterium animalis subsp. lactis DN-173 010 strain. A decrease in cecal pH and alterations in short chain fatty acid profiles occurred with consumption, and there were concomitant increases in the abundance of select lactate-consuming and butyrate-producing bacteria. These metabolic shifts created a nonpermissive environment for the Enterobacteriaceae recently identified as colitogenic in a T-bet−/−Rag2−/− ulcerative colitis mouse model. In addition, 16S rRNA-based analysis of the T-bet−/−Rag2−/−fecal microbiota suggest that the structure of the endogenous gut microbiota played a key role in shaping the host response to the bacterial strains studied herein. We have identified features of the gut microbiota, at the membership and functional level, associated with response to this B. lactis-containing fermented milk product, and therefore this model provides a framework for evaluating and optimizing probiotic-based functional foods.
* Enterobacteriaceae
* intestinal inflammation
* microbiota
* probiotics
* colitis
Footnotes
* 3 To whom correspondence should be addressed. E-mail: wgarrett@hsph.harvard.edu.
* Author contributions: P.V., A.K., J.E.T.v.H.V., A.O., L.H.G., and W.S.G. designed research; P.V., C.A.G., C.B., M.M., M.L.D., A.D., S.P., and W.S.G. performed research; P.V., C.A.G., C.B., M.M., M.L.D., A.D., J.E.T.v.H.V., J.N.G., A.O., and W.S.G. analyzed data; and P.V. and W.S.G. wrote the paper.
* Conflict of interest statement: L.H.G. is a member of the Board of Directors of and holds equity in the Bristol Myers Squibb Corporation. P.V., C.B., A.K., and J.E.T.v.H.V. are employees of and hold equity in Groupe Danone.
* Data deposition: The 454 pyrosequencing reads reported in this paper have been deposited in the NCBI Short Read Archive (accession no. SRX025834.5).
* This article is a PNAS Direct Submission.
* This article contains supporting information online at https://www.pnas.org/lookup/suppl/do...DCSupplemental.
Freely available online through the PNAS open access option.
-
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