[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]
Surveillance and molecular epidemiology of Klebsiella pneumoniae that produce carbapenemases; the first report of OXA-48-like enzymes in North America [PublishAheadOfPrint]
Lascols, C., Peirano, G., Hackel, M., Laupland, K. B., Pitout, J. D. D.
A study was designed to characterize non-repeat isolates of carbapenemase-producing K. pneumoniae obtained from SMART worldwide surveillance program during 2008-2009. Characterization was done by PCR and sequencing for bla<SUB>VIM</SUB>, bla<SUB>IMP</SUB>, bla<SUB>NDM</SUB>, bla<SUB>OXA</SUB>, bla<SUB>KPC</SUB>, plasmid-mediated quinolone resistance and virulence factors (VFs). Genetic relatedness was determined with PFGE using XbaI and multi-locus sequencing typing. A total of 110 isolates were included; 47 possess genes that encode for KPCs, 26 NDMs, 19 VIMs, 13 OXA-48-like and 5 IMPs. We identified 3 different major STs among 65% of isolates (i.e. ST11 [n=11], ST147 [n=23] and ST258 [n=38]. ST11 and ST147 producing OXA-48-like and NDMs, were found in Argentina, Turkey, Greece, Italy and India; ST258 producing KPCs, was present in USA, Israel, Greece and Puerto Rico. The major STs consisted of up to 4 different pulsotypes and each pulsotype had a specific geographical distribution. A new ST, named ST903 with bla<SUB>IMP-26</SUB> was identified in the Philippines while two bla<SUB>OXA-48</SUB>-positive isolates were detected in the USA. There were no significant differences in the distribution of the VFs between the isolates; all were positive for fimH, mrkD, wabG and ureA. This is the first report of OXA-48-like enzymes in North America. Our study highlights the importance of surveillance programs using molecular techniques as powerful tools to identify the importance of international sequence types.
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Surveillance and molecular epidemiology of Klebsiella pneumoniae that produce carbapenemases; the first report of OXA-48-like enzymes in North America [PublishAheadOfPrint]
Lascols, C., Peirano, G., Hackel, M., Laupland, K. B., Pitout, J. D. D.
A study was designed to characterize non-repeat isolates of carbapenemase-producing K. pneumoniae obtained from SMART worldwide surveillance program during 2008-2009. Characterization was done by PCR and sequencing for bla<SUB>VIM</SUB>, bla<SUB>IMP</SUB>, bla<SUB>NDM</SUB>, bla<SUB>OXA</SUB>, bla<SUB>KPC</SUB>, plasmid-mediated quinolone resistance and virulence factors (VFs). Genetic relatedness was determined with PFGE using XbaI and multi-locus sequencing typing. A total of 110 isolates were included; 47 possess genes that encode for KPCs, 26 NDMs, 19 VIMs, 13 OXA-48-like and 5 IMPs. We identified 3 different major STs among 65% of isolates (i.e. ST11 [n=11], ST147 [n=23] and ST258 [n=38]. ST11 and ST147 producing OXA-48-like and NDMs, were found in Argentina, Turkey, Greece, Italy and India; ST258 producing KPCs, was present in USA, Israel, Greece and Puerto Rico. The major STs consisted of up to 4 different pulsotypes and each pulsotype had a specific geographical distribution. A new ST, named ST903 with bla<SUB>IMP-26</SUB> was identified in the Philippines while two bla<SUB>OXA-48</SUB>-positive isolates were detected in the USA. There were no significant differences in the distribution of the VFs between the isolates; all were positive for fimH, mrkD, wabG and ureA. This is the first report of OXA-48-like enzymes in North America. Our study highlights the importance of surveillance programs using molecular techniques as powerful tools to identify the importance of international sequence types.
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