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Infect Genetics Evol. Infection of cats with atypical feline coronaviruses harbouring a truncated form of the canine type I non-structural ORF3 gene
[Source: Infection, Genetics and Evolution, full page: (LINK). Abstract, edited.]
Infection, Genetics and Evolution, Available online 9 October 2013
Infection of cats with atypical feline coronaviruses harbouring a truncated form of the canine type I non-structural ORF3 gene
Sophie Le Poder<SUP>a</SUP>, Anne-Laure Pham-**** d?Alexandry d?Orangiani<SUP>a</SUP>, Lidia Duarte<SUP>a</SUP>, Annie Fournier<SUP>a</SUP>, Cristina Horhogea<SUP>b</SUP>, Carine Pinhas<SUP>b</SUP>, Astrid Vabret<SUP>c</SUP>, Marc Eloit<SUP>a</SUP>
<SUP></SUP>
<SUP>a</SUP> Universit? Paris-Est, Ecole Nationale V?t?rinaire d?Alfort, UMR 1161 virologie INRA ENVA ANSES, Maisons-Alfort, F-94704, France <SUP>b</SUP> Universit? des Sciences Agricoles et M?decine V?t?rinaire Ion Ionescu de la Brad Iasi, Facult? de M?decine V?t?rinaire, Aleea Mihail Sadoveanu, Nr. 8, cod 700489, Iasi, Romania <SUP>c</SUP> Laboratoire de Virologie, EA 2128, CHU de Caen. Avenue Georges Clemenceau 14033 Caen cedex, France
Received 1 August 2013. Revised 21 September 2013. Accepted 26 September 2013 . Available online 9 October 2013.
Open Access
Highlights
Abstract
Feline and canine coronaviruses (FCoV and CCoV, respectively) are common pathogens of cats and dogs sometimes leading to lethal infections named feline infectious peritonitis (FIP) and canine pantropic coronavirus infection. FCoV and CCoV are each subdivided into two serotypes, FCoV-I/II and CCoV-I/II. A phylogenetic relationship is evident between, on one hand, CCoV-I/FCoV-I, and on the other hand, CCoV-II/ FCoV-II, suggesting that interspecies transmission can occur. The aim of the present study was to evaluate the prevalence of coronavirus (CoV)-infected cats according to their contact with dogs and to genetically analyze the CoV strains infecting cats. From 2003 to 2009, we collected 88 faecal samples from healthy cats and 11 ascitic fluids from FIP cats. We investigated the possible contact with dog in the household and collected dogs samples if appropriate. Out of 99 cat samples, 26 were coronavirus positive, with 6 cats living with at least one dog, thus showing that contact with dogs does not appear as a predisposing factor for cats CoV infections.
Molecular and phylogenetic analyses of FCoV strains were conducted using partial N and S sequences. Six divergent strains were identified with the N gene clustering with CCoV-I whereas the 3?end of S was related to FCoV-I. Further analysis on those 6 samples was attempted by researching the presence of the ORF3 gene, the latter being peculiar to CCoV-I to date. We succeeded to amplify the ORF3 gene in 5 samples out of 6. Thus, our data strongly suggest the circulation of atypical FCoV strains harbouring the CCoV-I ORF3 gene among cats. Moreover, the ORF3 genes recovered from the feline strains exhibited shared deletions, never described before, suggesting that these deletions could be critical in the adaptation of these strains to the feline host.
Key words: Canine coronavirus type I; Non-structural ORF3 gene; Feline coronavirus; Phylogenetic analysis
Copyright ? 2013 Elsevier B.V. All rights reserved. ScienceDirect? is a registered trademark of Elsevier B.V.
http://dx.doi.org/10.1016/j.meegid.2013.09.024
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Infection, Genetics and Evolution, Available online 9 October 2013
Infection of cats with atypical feline coronaviruses harbouring a truncated form of the canine type I non-structural ORF3 gene
Sophie Le Poder<SUP>a</SUP>, Anne-Laure Pham-**** d?Alexandry d?Orangiani<SUP>a</SUP>, Lidia Duarte<SUP>a</SUP>, Annie Fournier<SUP>a</SUP>, Cristina Horhogea<SUP>b</SUP>, Carine Pinhas<SUP>b</SUP>, Astrid Vabret<SUP>c</SUP>, Marc Eloit<SUP>a</SUP>
<SUP></SUP>
<SUP>a</SUP> Universit? Paris-Est, Ecole Nationale V?t?rinaire d?Alfort, UMR 1161 virologie INRA ENVA ANSES, Maisons-Alfort, F-94704, France <SUP>b</SUP> Universit? des Sciences Agricoles et M?decine V?t?rinaire Ion Ionescu de la Brad Iasi, Facult? de M?decine V?t?rinaire, Aleea Mihail Sadoveanu, Nr. 8, cod 700489, Iasi, Romania <SUP>c</SUP> Laboratoire de Virologie, EA 2128, CHU de Caen. Avenue Georges Clemenceau 14033 Caen cedex, France
Received 1 August 2013. Revised 21 September 2013. Accepted 26 September 2013 . Available online 9 October 2013.
Open Access
Highlights
- FCoV and CCoV have close genetic relationships.
- Molecular characterization of FCoV was conducted in cats living or not with dogs.
- Presence of dogs in households does not predispose to coronavirus infection of cats.
- Identification of atypical FCoV strains harbouring the CCoV-I ORF3 gene is reported.
- ORF3 genes recovered from infected cats exhibited shared deletions never described.
Abstract
Feline and canine coronaviruses (FCoV and CCoV, respectively) are common pathogens of cats and dogs sometimes leading to lethal infections named feline infectious peritonitis (FIP) and canine pantropic coronavirus infection. FCoV and CCoV are each subdivided into two serotypes, FCoV-I/II and CCoV-I/II. A phylogenetic relationship is evident between, on one hand, CCoV-I/FCoV-I, and on the other hand, CCoV-II/ FCoV-II, suggesting that interspecies transmission can occur. The aim of the present study was to evaluate the prevalence of coronavirus (CoV)-infected cats according to their contact with dogs and to genetically analyze the CoV strains infecting cats. From 2003 to 2009, we collected 88 faecal samples from healthy cats and 11 ascitic fluids from FIP cats. We investigated the possible contact with dog in the household and collected dogs samples if appropriate. Out of 99 cat samples, 26 were coronavirus positive, with 6 cats living with at least one dog, thus showing that contact with dogs does not appear as a predisposing factor for cats CoV infections.
Molecular and phylogenetic analyses of FCoV strains were conducted using partial N and S sequences. Six divergent strains were identified with the N gene clustering with CCoV-I whereas the 3?end of S was related to FCoV-I. Further analysis on those 6 samples was attempted by researching the presence of the ORF3 gene, the latter being peculiar to CCoV-I to date. We succeeded to amplify the ORF3 gene in 5 samples out of 6. Thus, our data strongly suggest the circulation of atypical FCoV strains harbouring the CCoV-I ORF3 gene among cats. Moreover, the ORF3 genes recovered from the feline strains exhibited shared deletions, never described before, suggesting that these deletions could be critical in the adaptation of these strains to the feline host.
Key words: Canine coronavirus type I; Non-structural ORF3 gene; Feline coronavirus; Phylogenetic analysis
Copyright ? 2013 Elsevier B.V. All rights reserved. ScienceDirect? is a registered trademark of Elsevier B.V.
http://dx.doi.org/10.1016/j.meegid.2013.09.024
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