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Detection of SARS-CoV-2 in raw and treated wastewater in Germany

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  • Detection of SARS-CoV-2 in raw and treated wastewater in Germany

    Science of the Total Environment
    Volume 751; 10 January 2021

    Detection of SARS-CoV-2 in raw and treated wastewater in Germany – Suitability for COVID-19 surveillance and potential transmission risks

    AndreasWeber,SabrinaSchiwy,VolkerLinnemann,MarkusB rinkmann,MarekWidera,CarolaGreve,AxelJanke,HennerH ollert,ThomasWintgens,SandraCiesek

    Highlights
    •The first study that reports the detection of SARS-CoV-2 in wastewater in Germany using RT-qPCR.
    •The presence of SARS-CoV-2 was confirmed by sequencing, but also the risk of false-positive results has been elucidated.
    •In raw wastewater, 3.0 to 20 gene equivalents/mL was found in raw wastewater.
    •The replication potential tests were negative for wastewater samples.
    •Sanger sequencing was required to differentiate the genetic pattern clearly.

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