Source: http://news.china.com.cn/2020-01/30/...t_75657814.htm
Uninterrupted "battle" 24 hours a day-visiting Beijing CDC virus detection laboratory
Published: 2020-01-30 06:14:47 | Source: Xinhua | Author: Xia g
Xinhua News Agency, Beijing, January 29th. There is such a group of people who, although not in the clinical field, are fighting "face-to-face" with the virus; they are in an invisible "front" and face an invisible "enemy" ; They keep the virus out of nowhere and protect the health of a city.
They have a common name-"CDC."
Since the outbreak of pneumonia caused by the new coronavirus infection in Wuhan, China has shown a spreading trend. Surveillance of the epidemic, research of viruses, screening of suspected cases, follow-up of close contacts ... they have never stopped.
On the morning of the 29th, the reporter came to the Beijing Centers for Disease Control and Prevention of Infectious Diseases and Endemic Disease Control, and followed the laboratory staff's visit to the whole process of nucleic acid detection of the new coronavirus.
The Beijing Municipal Center for Disease Control and Prevention has five specialized groups including respiratory infectious diseases, intestinal infectious diseases, and natural epidemic diseases. There are 61 staff members in addition to the Beijing area. In addition to the prevention and control of some infectious diseases such as influenza, parasitic diseases and illegal infectious diseases, they also shoulder the prevention and control of endemic diseases and newly emerging infectious diseases.
When he came to the pathogen detection laboratory area on the fifth floor of the disease prevention building, the reporter put on masks, rubber gloves, disposable isolation clothing, isolation caps, and shoe covers as required, and stepped in under the guidance of laboratory staff Pan Yang. The experimental detection area. Along the corridor, the reporter saw that it was divided into 6 separate virus detection laboratories, 2 serum laboratories, 1 tissue culture room, and 1 molecular biology laboratory.
Since the outbreak of pneumonia caused by the new coronavirus, laboratory virus testing has been ongoing for 24 hours. Samples are continuously sent, and technicians adhere to nucleic acid detection and review 24 hours a day in order to determine in time whether there is a new type of coronavirus in the sample.
In the "Second Generation Sequencing Lab", two second generation sequencing instruments are on standby at any time. "Through deep sequencing pathogenic examination, you can find a new coronavirus RNA sequence, which is equivalent to confirming the virus'" identity card "so that it can be accurately identified. Pan Yang said that at the stage when the virus was just discovered, nucleic acid detection and gene sequencing Method cross-validation can maximize the accuracy of diagnosis.
Pan Yang told reporters that after taking the patient's respiratory tract samples, they will be stored in a special virus sampling tube, and then used a special container for two- and three-layer packaging. All samples will be placed in a special aluminum sample shipping box To ensure the safety of sample transportation.
"Every study of the virus is like a battle." Pan Yang said that one of the most meticulous key steps in the detection process is sample processing. The processing process is carried out by a tester wearing a full set of personal protective equipment with the help of a biological safety cabinet. Finished. The biological safety cabinet is an air-purifying negative pressure cabinet, so that the virus cannot be spread outside the biological cabinet. With the help of multiple filtrations and various chemical reagents, the coat of the virus was gradually peeled off to expose the nucleic acids. After the nucleic acid is extracted, it is sent to the gene amplification instrument to observe whether the virus is present in the sample by observing the amplification process.
Wang Quanyi, director of the Institute of Infectious Diseases and Endemic Disease Control of the Beijing Centers for Disease Control and Prevention, said that the new coronavirus test was performed according to the report of the receiving medical institution, and was reviewed by the primary screening laboratory and the city center for disease control. Confirm by means of confirmation. In order to further improve the efficiency, the urban CDC laboratory testing process has been changed from “series” to “parallel”. After the improvement, the test time can be saved by 6 to 8 hours, which can more accurately and quickly diagnose patients.
Wang Quanyi introduced that there are 55 infectious disease network laboratories in Beijing, which are composed of disease control centers and hospitals. At present, the network laboratories that comply with biosafety are being launched one after another, and the detection process will be further optimized.
Uninterrupted "battle" 24 hours a day-visiting Beijing CDC virus detection laboratory
Published: 2020-01-30 06:14:47 | Source: Xinhua | Author: Xia g
Xinhua News Agency, Beijing, January 29th. There is such a group of people who, although not in the clinical field, are fighting "face-to-face" with the virus; they are in an invisible "front" and face an invisible "enemy" ; They keep the virus out of nowhere and protect the health of a city.
They have a common name-"CDC."
Since the outbreak of pneumonia caused by the new coronavirus infection in Wuhan, China has shown a spreading trend. Surveillance of the epidemic, research of viruses, screening of suspected cases, follow-up of close contacts ... they have never stopped.
On the morning of the 29th, the reporter came to the Beijing Centers for Disease Control and Prevention of Infectious Diseases and Endemic Disease Control, and followed the laboratory staff's visit to the whole process of nucleic acid detection of the new coronavirus.
The Beijing Municipal Center for Disease Control and Prevention has five specialized groups including respiratory infectious diseases, intestinal infectious diseases, and natural epidemic diseases. There are 61 staff members in addition to the Beijing area. In addition to the prevention and control of some infectious diseases such as influenza, parasitic diseases and illegal infectious diseases, they also shoulder the prevention and control of endemic diseases and newly emerging infectious diseases.
When he came to the pathogen detection laboratory area on the fifth floor of the disease prevention building, the reporter put on masks, rubber gloves, disposable isolation clothing, isolation caps, and shoe covers as required, and stepped in under the guidance of laboratory staff Pan Yang. The experimental detection area. Along the corridor, the reporter saw that it was divided into 6 separate virus detection laboratories, 2 serum laboratories, 1 tissue culture room, and 1 molecular biology laboratory.
Since the outbreak of pneumonia caused by the new coronavirus, laboratory virus testing has been ongoing for 24 hours. Samples are continuously sent, and technicians adhere to nucleic acid detection and review 24 hours a day in order to determine in time whether there is a new type of coronavirus in the sample.
In the "Second Generation Sequencing Lab", two second generation sequencing instruments are on standby at any time. "Through deep sequencing pathogenic examination, you can find a new coronavirus RNA sequence, which is equivalent to confirming the virus'" identity card "so that it can be accurately identified. Pan Yang said that at the stage when the virus was just discovered, nucleic acid detection and gene sequencing Method cross-validation can maximize the accuracy of diagnosis.
Pan Yang told reporters that after taking the patient's respiratory tract samples, they will be stored in a special virus sampling tube, and then used a special container for two- and three-layer packaging. All samples will be placed in a special aluminum sample shipping box To ensure the safety of sample transportation.
"Every study of the virus is like a battle." Pan Yang said that one of the most meticulous key steps in the detection process is sample processing. The processing process is carried out by a tester wearing a full set of personal protective equipment with the help of a biological safety cabinet. Finished. The biological safety cabinet is an air-purifying negative pressure cabinet, so that the virus cannot be spread outside the biological cabinet. With the help of multiple filtrations and various chemical reagents, the coat of the virus was gradually peeled off to expose the nucleic acids. After the nucleic acid is extracted, it is sent to the gene amplification instrument to observe whether the virus is present in the sample by observing the amplification process.
Wang Quanyi, director of the Institute of Infectious Diseases and Endemic Disease Control of the Beijing Centers for Disease Control and Prevention, said that the new coronavirus test was performed according to the report of the receiving medical institution, and was reviewed by the primary screening laboratory and the city center for disease control. Confirm by means of confirmation. In order to further improve the efficiency, the urban CDC laboratory testing process has been changed from “series” to “parallel”. After the improvement, the test time can be saved by 6 to 8 hours, which can more accurately and quickly diagnose patients.
Wang Quanyi introduced that there are 55 infectious disease network laboratories in Beijing, which are composed of disease control centers and hospitals. At present, the network laboratories that comply with biosafety are being launched one after another, and the detection process will be further optimized.
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